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AB250663

Anti-SIKE1 antibody [EPR14692] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SIKE1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

SIKE, SIKE1, Suppressor of IKBKE 1, Suppressor of IKK-epsilon

1 Images
Western blot - Anti-SIKE1 antibody [EPR14692] - BSA and Azide free (AB250663)
  • WB

Supplier Data

Western blot - Anti-SIKE1 antibody [EPR14692] - BSA and Azide free (AB250663)

This data was developed using the same antibody clone in a different buffer formulation (ab183509).

Lanes 1-3 : Merged signal (red and green). Green - ab183509 observed at 25 kDa. Red - loading control ab8245 observed at 37 kDa.

ab183509 Rabbit monoclonal [EPR14692] to SIKE1 was shown to specifically react with Suppressor of IKBKE 1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265933 (knockout cell lysate ab258188) was used. Wild-type and Suppressor of IKBKE 1 knockout samples were subjected to SDS-PAGE. ab183509 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SIKE1 antibody [EPR14692] (<a href='/en-us/products/primary-antibodies/sike1-antibody-epr14692-ab183509'>ab183509</a>) at 1/10000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

SIKE1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human SIKE1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-sike1-knockout-hela-cell-line-ab265933'>ab265933</a>)

Lane 3:

HEK-293 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14692

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Primary Antibodies

AB180575

Anti-SAMD9 antibody [EPR13603]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-SAMD9 antibody [EPR13603] (AB180575)

  • 0
  • 0
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5
Secondary Antibodies

AB99811

Mouse monoclonal [JDC-12] Anti-Human lambda light chain (HRP)

VeriBlot

Indirect ELISA - Mouse monoclonal [JDC-12] Anti-Human lambda light chain (HRP) (AB99811)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab250663 is the carrier-free version of ab183509.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIKE1 also known as Squamous Cell Carcinoma Antigen Recognized by T-cells 3 (SART3) is a protein with a molecular weight of approximately 24 kDa. It inhibits the kinase activity of Tank-Binding Kinase 1 (TBK1) and IκB kinase ε (IKKε) which are critical for the induction of type I interferons and other cytokines in response to viral infections. SIKE1 is primarily expressed in various tissues including the brain and liver though expression levels may vary depending on cellular context.
Biological function summary

SIKE1 interacts with signaling pathways to modulate immune responses. As a negative regulator of the interferon signaling pathway it forms complexes with TBK1/IKKε to suppress excessive immune activation preventing potential tissue damage. By efficiently regulating kinase activity SIKE1 helps maintain balance in immune signaling ensuring proper immune defense while avoiding overactivation.

Pathways

The involvement of SIKE1 extends to the toll-like receptor (TLR) and retinoic acid-inducible gene I (RIG-I)-like receptor signaling pathways. These pathways play essential roles in detecting and responding to viral pathogens. SIKE1 modulates the activity of TBK1 and IKKε which are central to the aforementioned pathways impacting downstream molecules like IRF3 and NF-kB that drive interferon production and inflammatory responses.

SiKE1's regulatory role relates to conditions characterized by dysregulated immune responses. For instance in viral infections where excessive interferon production occurs SIKE1 can mitigate potential tissue damage by dampening cytokine output. It is also studied in auto-inflammatory disorders where control of cytokine production is important for managing disease severity. Its connection to TBK1 and IKKε further implicates SIKE1 in the context of these immune-related conditions as these kinases are targets for therapeutic intervention in disorders related to immune dysregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and RIGI. Does not inhibit NF-kappa-B activation pathways (PubMed : 16281057). Associates with the striatin-interacting phosphatase and kinase (STRIPAK) core complex, forming the extended (SIKE1 : SLMAP)STRIPAK complex (PubMed : 30622739). The (SIKE1 : SLMAP)STRIPAK complex dephosphorylates STK3 leading to the inhibition of Hippo signaling and the control of cell growth (PubMed : 30622739).
See full target information SIKE1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 18:20230639 PubMed36820063

2023

Glycoprotein non-metastatic melanoma B interacts with epidermal growth factor receptor to regulate neural stem cell survival and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Yang,Gang Jin,Shihong Chen,Jing Luo,Wei Xu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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