AB5639

Anti-SIL1 antibody

Name: Anti-SIL1 antibody (ab5639) | Abcam
Brand: Abcam Limited
SKU: AB5639

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(7 Publications)

Goat Polyclonal SIL1 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human SIL1 aa 400 to C-terminus.

View Alternative Names

UNQ545/PRO836, SIL1, Nucleotide exchange factor SIL1, BiP-associated protein, BAP

1 Images

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Western blot - Anti-SIL1 antibody (AB5639)

ab5639 staining (1µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

All lanes:

Western blot - Anti-SIL1 antibody (ab5639)

Predicted band size: 52 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human SIL1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H173

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" } } }

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Product details

SIL1 is a resident endoplasmic reticulum (ER) glycoprotein that interacts with the ATPase domain of BIP and enhances nucleotide exchange.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SIL1 protein known also as nucleotide exchange factor SIL1 or BAP plays a mechanical role as an endoplasmic reticulum (ER)-localized nucleotide exchange factor for BiP a heat shock protein. SIL1 has a molecular mass of approximately 53 kDa. The expression of SIL1 occurs mainly in tissues with high secretory demand such as the pancreas and liver. It exists prominently within the lumen of the ER where it stabilizes protein folding by facilitating nucleotide exchange on BiP.

Biological function summary

SIL1 operates as a critical component for ER homeostasis and proteostasis. It assists the chaperone function of BiP by promoting ADP/ATP exchange which is an essential step in the protein folding cycle. SIL1 forms part of a complex network of proteins within the ER that regulate protein biosynthesis folding and degradation. Its interactive network ensures efficient ER functioning affecting the maturation and stability of secretory and membrane proteins.

Pathways

SIL1 interacts within both the unfolded protein response (UPR) and ER-associated degradation (ERAD) pathways. In the UPR SIL1's role in BiP activity ensures proper folding of nascent polypeptides reducing ER stress. In ERAD SIL1's actions facilitate misfolded protein processing and clearance. SIL1 is closely related to other chaperones and co-chaperones like GRP78 with whom it collaboratively maintains ER function under stress conditions ensuring cellular homeostasis.

SIL1 mutations associate with Marinesco-Sjögren syndrome a rare autosomal recessive disorder. The syndrome results from disrupted protein homeostasis in the ER leading to multisystem clinical symptoms such as muscle weakness and cataracts. SIL1 also connects to neurodegenerative diseases often characterized by ER stress and faulty proteostasis implicating proteins such as BiP whose impairment also results from SIL1 malfunctions. Understanding these connections is essential for identifying potential therapeutic targets for these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.

See full target information SIL1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Science progress 103:36850419891046 PubMed31791191

2019

Nucleotide exchange factor SIL1 promotes the progress of breast cancer cells via regulating the cell cycle and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Feng Li,Wei-Wei Xu,Ji-Dan Li,Feng-Ling Tao,Jian-Xin Chen,Jin-Hua Xu

Frontiers in neurology 10:562 PubMed31258504

2019

Identification of Cellular Pathogenicity Markers for SIL1 Mutations Linked to Marinesco-Sjögren Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Gatz,Denisa Hathazi,Ute Münchberg,Stephan Buchkremer,Thomas Labisch,Ben Munro,Rita Horvath,Ana Töpf,Joachim Weis,Andreas Roos

OncoTargets and therapy 11:3775-3783 PubMed29997438

2018

SIL1 functions as an oncogene in glioma by AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Xu,Shangchen Xu,Rui Zhang,Tao Xin,Qi Pang

Nature neuroscience 18:227-38 PubMed25559081

2015

Marinesco-Sjögren syndrome protein SIL1 regulates motor neuron subtype-selective ER stress in ALS.

Applications

IHC-P, IHC-FoFr, IHC-FoFr, IHC-P

Species

Human, Human, Mouse, Mouse

Audrey Filézac de L'Etang,Niran Maharjan,Marisa Cordeiro Braña,Céline Ruegsegger,Ruth Rehmann,Anand Goswami,Andreas Roos,Dirk Troost,Bernard L Schneider,Joachim Weis,Smita Saxena

European journal of human genetics : EJHG 16:961-9 PubMed18285827

2008

Novel SIL1 mutations and exclusion of functional candidate genes in Marinesco-Sjögren syndrome.

Applications

ICC/IF

Species

Mouse

Anna-Kaisa Anttonen,Eija Siintola,Lisbeth Tranebjaerg,Nobue K Iwata,Emilia K Bijlsma,Hiroyuki Meguro,Yaeko Ichikawa,Jun Goto,Outi Kopra,Anna-Elina Lehesjoki

Molecular endocrinology (Baltimore, Md.) 21:376-87 PubMed17095577

2006

The orphan receptor tyrosine kinase Ror2 promotes osteoblast differentiation and enhances ex vivo bone formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liu,Ramesh A Bhat,Laura M Seestaller-Wehr,Shoichi Fukayama,Annamarie Mangine,Robert A Moran,Barry S Komm,Peter V N Bodine,Julia Billiard

Nature genetics 37:1309-11 PubMed16282978

2005

The gene disrupted in Marinesco-Sjögren syndrome encodes SIL1, an HSPA5 cochaperone.

Applications

IHC-Fr

Species

Human

Anna-Kaisa Anttonen,Ibrahim Mahjneh,Riikka H Hämäläinen,Clotilde Lagier-Tourenne,Outi Kopra,Laura Waris,Mikko Anttonen,Tarja Joensuu,Hannu Kalimo,Anders Paetau,Lisbeth Tranebjaerg,Denys Chaigne,Michel Koenig,Orvar Eeg-Olofsson,Bjarne Udd,Mirja Somer,Hannu Somer,Anna-Elina Lehesjoki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SIL1 antibody

Western blot - Anti-SIL1 antibody (AB5639)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise