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AB126863

Anti-SIM1 antibody

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Goat Polyclonal SIM1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SIM1 aa 500-600.

View Alternative Names

BHLHE14, SIM1, Single-minded homolog 1, Class E basic helix-loop-helix protein 14, bHLHe14

1 Images
Western blot - Anti-SIM1 antibody (AB126863)
  • WB

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Western blot - Anti-SIM1 antibody (AB126863)

All lanes:

Western blot - Anti-SIM1 antibody (ab126863) at 1 µg/mL

All lanes:

HEK293 lysate at 35 µg

Predicted band size: 86 kDa

true

Exposure time: 1hr

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SIM1 aa 500-600. The exact immunogen used to generate this antibody is proprietary information.

P81133

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIM1 also known as Single-minded homolog 1 is a transcription factor with a mass of approximately 83 kDa. This protein plays a mechanical role in regulating gene expression by binding to specific DNA sequences through its basic helix-loop-helix (bHLH) and PAS domains. SIM1 is expressed in several tissues including the hypothalamus where it contributes to the development of specific neuronal populations.
Biological function summary

The role of SIM1 extends to neurodevelopment where it interacts with other transcription factors to form complexes essential for brain functioning. Notably SIM1 is critical for the proper formation and differentiation of neurons in the paraventricular nucleus of the hypothalamus which are important for physiological regulation and neuroendocrine functions. Through these interactions SIM1 influences various processes related to behavior and homeostasis.

Pathways

The role of SIM1 becomes evident in developmental and signaling pathways. It is involved in the hypothalamic-pituitary axis where it interacts with melanocortin 4 receptor (MC4R) signaling. These pathways are significant for regulating food intake and energy expenditure. SIM1 also has associations with the Fibroblast Growth Factor (FGF) pathway influencing the terminal differentiation of specific neuron types.

The dysfunction of SIM1 can lead to conditions such as obesity and developmental brain disorders. Mutations or altered expression of SIM1 link it to obesity emphasizing its connection with energy balance and weight control through its interaction with MC4R. Furthermore abnormalities in SIM1 expression may relate to idiopathic hypogonadotropic hypogonadism associated with disrupted interactions with other hypothalamic factors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional factor that may have pleiotropic effects during embryogenesis and in the adult.
See full target information SIM1
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