AB131161

Anti-SIM2 antibody [EPR7878]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal SIM2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BHLHE15, SIM2, Single-minded homolog 2, Class E basic helix-loop-helix protein 15, bHLHe15

2 Images

Unknown

Western blot - Anti-SIM2 antibody [EPR7878] (AB131161)

All lanes:

Western blot - Anti-SIM2 antibody [EPR7878] (ab131161) at 1/1000 dilution

Lane 1:

Human fetal forebrain lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 73 kDa

Observed band size: 64 kDa

false

Unknown

Western blot - Anti-SIM2 antibody [EPR7878] (AB131161)

All lanes:

Western blot - Anti-SIM2 antibody [EPR7878] (ab131161) at 1/1000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 73 kDa

Observed band size: 64 kDa

false

Carrier free

Anti-SIM2 antibody [EPR7878] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7878

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIM2 also known as Single-minded homolog 2 is a transcription factor belonging to the bHLH-PAS family. It possesses a molecular weight of approximately 88 kDa. SIM2 plays a significant role in regulating gene expression by binding to the DNA hence influencing various biological processes. This protein is predominantly expressed in fetal and adult brain tissues as well as in other tissues including the kidney and lung suggesting its involvement in diverse physiological functions.

Biological function summary

This transcription factor modulates neural development and is implicated in the suppression of tumorigenesis. SIM2 interacts with ARNT (aryl hydrocarbon receptor nuclear translocator) to form a transcriptionally active complex. This complex plays a critical function in maintaining the delicate balance of cellular proliferation and differentiation. SIM2's expression levels change in response to developmental cues indicating its importance in development especially within the nervous system.

Pathways

This protein participates in the hypoxia and circadian rhythms pathways. In the hypoxia pathway SIM2 influences the cellular response to low oxygen levels by interacting with other transcription factors like HIF-1α while in the circadian rhythms pathway it affects the regulation of genes involved in maintaining the body's biological clock. These pathways exhibit how SIM2 integrates external signals demonstrating its function in complex biological networks.

This transcription factor has been associated with Down syndrome and certain types of cancer such as prostate cancer. The downregulation or overexpression of SIM2 can contribute to the aberrant cellular proliferation characteristic of these disorders. Its interaction with other proteins such as ERG in prostate cancer links SIM2 to the progression and pathophysiology of these diseases highlighting its potential as a therapeutic target in understanding and treating these complex conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.

See full target information SIM2

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 45:1059-1071 PubMed33650646

2021

Long non‑coding RNA CASC15 facilitates esophageal squamous cell carcinoma tumorigenesis via decreasing SIM2 stability via FTO‑mediated demethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Qin,Meng Dong,Zhengyang Wang,Jiajia Wan,Yingying Xie,Yi Jiao,Dan Yan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SIM2 antibody [EPR7878]

Western blot - Anti-SIM2 antibody [EPR7878] (AB131161)

Western blot - Anti-SIM2 antibody [EPR7878] (AB131161)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise