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AB248363

Anti-SIM2 antibody [EPR7878] - BSA and Azide free

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Rabbit Recombinant Monoclonal SIM2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

BHLHE15, SIM2, Single-minded homolog 2, Class E basic helix-loop-helix protein 15, bHLHe15

2 Images
Western blot - Anti-SIM2 antibody [EPR7878] - BSA and Azide free (AB248363)
  • WB

Unknown

Western blot - Anti-SIM2 antibody [EPR7878] - BSA and Azide free (AB248363)

This data was developed using ab131161, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SIM2 antibody [EPR7878] (<a href='/en-us/products/primary-antibodies/sim2-antibody-epr7878-ab131161'>ab131161</a>) at 1/1000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 73 kDa

Observed band size: 64 kDa

false

Western blot - Anti-SIM2 antibody [EPR7878] - BSA and Azide free (AB248363)
  • WB

Unknown

Western blot - Anti-SIM2 antibody [EPR7878] - BSA and Azide free (AB248363)

This data was developed using ab131161, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SIM2 antibody [EPR7878] (<a href='/en-us/products/primary-antibodies/sim2-antibody-epr7878-ab131161'>ab131161</a>) at 1/1000 dilution

Lane 1:

Human fetal forebrain lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 73 kDa

Observed band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7878

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248363 is the carrier-free version of ab131161.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIM2 also known as Single-minded homolog 2 is a transcription factor belonging to the bHLH-PAS family. It possesses a molecular weight of approximately 88 kDa. SIM2 plays a significant role in regulating gene expression by binding to the DNA hence influencing various biological processes. This protein is predominantly expressed in fetal and adult brain tissues as well as in other tissues including the kidney and lung suggesting its involvement in diverse physiological functions.
Biological function summary

This transcription factor modulates neural development and is implicated in the suppression of tumorigenesis. SIM2 interacts with ARNT (aryl hydrocarbon receptor nuclear translocator) to form a transcriptionally active complex. This complex plays a critical function in maintaining the delicate balance of cellular proliferation and differentiation. SIM2's expression levels change in response to developmental cues indicating its importance in development especially within the nervous system.

Pathways

This protein participates in the hypoxia and circadian rhythms pathways. In the hypoxia pathway SIM2 influences the cellular response to low oxygen levels by interacting with other transcription factors like HIF-1α while in the circadian rhythms pathway it affects the regulation of genes involved in maintaining the body's biological clock. These pathways exhibit how SIM2 integrates external signals demonstrating its function in complex biological networks.

This transcription factor has been associated with Down syndrome and certain types of cancer such as prostate cancer. The downregulation or overexpression of SIM2 can contribute to the aberrant cellular proliferation characteristic of these disorders. Its interaction with other proteins such as ERG in prostate cancer links SIM2 to the progression and pathophysiology of these diseases highlighting its potential as a therapeutic target in understanding and treating these complex conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.
See full target information SIM2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com