Rabbit Polyclonal SIN1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MAPKAP1.
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- Proceedings of the National Academy of Sciences of 109:1667-722012Glycerolipid signals alter mTOR complex 2 (mTORC2) to diminish insulin signaling.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MAPKAP1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BPZ7
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.5 µg/mL | Notes - |
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Target data
Function
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.
Alternative names
MIP1, SIN1, MAPKAP1, Target of rapamycin complex 2 subunit MAPKAP1, TORC2 subunit MAPKAP1, Mitogen-activated protein kinase 2-associated protein 1, Stress-activated map kinase-interacting protein 1, SAPK-interacting protein 1, mSIN1
Recommended products
Rabbit Polyclonal SIN1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MAPKAP1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MAPKAP1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BPZ7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SIN1 also known as MAPKAP1 or mTORC2 component is a protein with a mass of approximately 66 kDa. It is an essential part of the mTOR complex 2 (mTORC2) a component critical for controlling cell growth and metabolism. SIN1 is expressed in various tissues indicating its broad role in numerous cellular processes. This expression includes key sites like the liver heart and muscle tissues.
- Biological function summary
SIN1 plays an important role in regulating mTORC2 which participates in the phosphorylation of Akt (Protein Kinase B). It forms part of the mTORC2 complex along with mTOR (mechanistic target of rapamycin) and RICTOR (Rapamycin-Insensitive Companion of mTOR). This complex influences cytoskeletal organization cell survival and nutrient sensing important for many cellular functions.
- Pathways
SIN1 involvement in mTOR signaling and PI3K-Akt pathways highlights its significance. These pathways are vital for cell growth proliferation and survival. Through mTORC2 it regulates Akt phosphorylation impacting downstream processes including processes controlled by proteins like SGK (serum and glucocorticoid-regulated kinase). SIN1's interactions within these pathways highlight its central role in cell signaling networks.
- Associated diseases and disorders
SIN1 shows links to cancer and diabetes pointing to its influence in disease progression. Alterations in the mTOR signaling pathway involving SIN1 can lead to dysregulated cell growth common in cancerous states. In diabetes the mTOR pathway combined with proteins like insulin receptors affects insulin signaling and glucose homeostasis making SIN1 a target of interest for therapeutic research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIN1 antibody (ab64188)
ab64188 (2.5ug/ml) staining human SIN1 in human brain, cortex tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.
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