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AB277787

Anti-SIN1 (phospho T86) antibody [19HCLC]

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Rabbit Recombinant Multiclonal SIN1 phospho T86 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MAPKAP1 phospho T86 aa 50-100.

View Alternative Names

MIP1, SIN1, MAPKAP1, Target of rapamycin complex 2 subunit MAPKAP1, TORC2 subunit MAPKAP1, Mitogen-activated protein kinase 2-associated protein 1, Stress-activated map kinase-interacting protein 1, SAPK-interacting protein 1, mSIN1

1 Images
Western blot - Anti-SIN1 (phospho T86) antibody [19HCLC] (AB277787)
  • WB

Supplier Data

Western blot - Anti-SIN1 (phospho T86) antibody [19HCLC] (AB277787)

All lanes:

Western blot - Anti-SIN1 (phospho T86) antibody [19HCLC] (ab277787) at 2 µg/mL

Lane 1:

A549 whole cell extracts at 30 µg

Lane 2:

A549 treated with Insulin (Serum starved for 24hrs, 100nM-10min) whole cell extracts at 30 µg

Lane 3:

PC-3 whole cell extracts at 30 µg

Lane 4:

PC-3 treated with Insulin (Serum starved for 24hrs, 100nM-10min) whole cell extracts at 30 µg

Lane 5:

A549 whole cell extracts at 30 µg with Phosphopeptide

Lane 6:

A549 treated with Insulin (Serum starved for 24hrs, 100nM-10min) whole cell extracts at 30 µg with Phosphopeptide

Lane 7:

PC-3 whole cell extract at 30 µg with Phosphopeptide

Lane 8:

PC-3 treated with Insulin (Serum starved for 24hrs, 100nM-10min) whole cell extract at 30 µg with Phosphopeptide

Secondary

All lanes:

HRP conjugate Goat anti-Rabbit IgG (H+L) Superclonal, 0.4 µg/mL at 1/2500 dilution

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

19HCLC

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human MAPKAP1 phospho T86 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q9BPZ7

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Reactivity data

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: 99.91% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIN1 also known as MAPKAP1 or mTORC2 component is a protein with a mass of approximately 66 kDa. It is an essential part of the mTOR complex 2 (mTORC2) a component critical for controlling cell growth and metabolism. SIN1 is expressed in various tissues indicating its broad role in numerous cellular processes. This expression includes key sites like the liver heart and muscle tissues.
Biological function summary

SIN1 plays an important role in regulating mTORC2 which participates in the phosphorylation of Akt (Protein Kinase B). It forms part of the mTORC2 complex along with mTOR (mechanistic target of rapamycin) and RICTOR (Rapamycin-Insensitive Companion of mTOR). This complex influences cytoskeletal organization cell survival and nutrient sensing important for many cellular functions.

Pathways

SIN1 involvement in mTOR signaling and PI3K-Akt pathways highlights its significance. These pathways are vital for cell growth proliferation and survival. Through mTORC2 it regulates Akt phosphorylation impacting downstream processes including processes controlled by proteins like SGK (serum and glucocorticoid-regulated kinase). SIN1's interactions within these pathways highlight its central role in cell signaling networks.

SIN1 shows links to cancer and diabetes pointing to its influence in disease progression. Alterations in the mTOR signaling pathway involving SIN1 can lead to dysregulated cell growth common in cancerous states. In diabetes the mTOR pathway combined with proteins like insulin receptors affects insulin signaling and glucose homeostasis making SIN1 a target of interest for therapeutic research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.
See full target information MAPKAP1 phospho T86
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