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AB101843

Anti-Sin3b antibody

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(1 Publication)

Rabbit Polyclonal SIN3B antibody. Suitable for WB, IP, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SIN3B aa 1-250.

View Alternative Names

KIAA0700, SIN3B, Paired amphipathic helix protein Sin3b, Histone deacetylase complex subunit Sin3b, Transcriptional corepressor Sin3b

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Sin3b antibody (AB101843)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sin3b antibody (AB101843)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling SIN3B protein at nucleus using ab101843 at a 1/500 dilution (Green).
phalloidin, a cytoskeleton marker, was stained at a 1/200 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue). Scale bar= 10µm.

Immunoprecipitation - Anti-Sin3b antibody (AB101843)
  • IP

Supplier Data

Immunoprecipitation - Anti-Sin3b antibody (AB101843)

Immunoprecipitation of SIN3B protein from HCT-116 whole cell extracts using 5 µg of SIN3B antibody.
Western blot analysis was performed using SIN3B antibody diluted at 1/500 dilution.

All lanes:

Immunoprecipitation - Anti-Sin3b antibody (ab101843) at 5 µg

Lane 1:

HCT-116 whole cell extracts

Lane 2:

Control with IgG

Lane 3:

Immunoprecipitated HCT116 whole cell extracts using ab101843

Secondary

All lanes:

Anti-Rabbit IgG

Predicted band size: 133 kDa

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Western blot - Anti-Sin3b antibody (AB101843)
  • WB

Unknown

Western blot - Anti-Sin3b antibody (AB101843)

10% SDS PAGE

All lanes:

Western blot - Anti-Sin3b antibody (ab101843) at 1/1000 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 133 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human SIN3B aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

O75182

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription. With FOXK1, regulates cell cycle progression probably by repressing cell cycle inhibitor genes expression. As part of the SIN3B complex represses transcription and counteracts the histone acetyltransferase activity of EP300 through the recognition H3K27ac marks by PHF12 and the activity of the histone deacetylase HDAC2 (PubMed : 37137925). SIN3B complex is recruited downstream of the constitutively active genes transcriptional start sites through interaction with histones and mitigates histone acetylation and RNA polymerase II progression within transcribed regions contributing to the regulation of transcription (PubMed : 21041482).
See full target information SIN3B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 10:e0127681 PubMed25993409

2015

Hairy/Enhancer-of-Split MEGANE and Proneural MASH1 Factors Cooperate Synergistically in Midbrain GABAergic Neurogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Clara-Zoe Wende,Saida Zoubaa,Alexandra Blak,Diego Echevarria,Salvador Martinez,François Guillemot,Wolfgang Wurst,Jordi Guimera
View all publications
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Product promise

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