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AB113657

Anti-SIPA1L3 antibody

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(1 Publication)

Rabbit Polyclonal SIPA1L3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SIPA1L3 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KIAA0545, SPAL3, SIPA1L3, Signal-induced proliferation-associated 1-like protein 3, SIPA1-like protein 3, SPA-1-like protein 3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIPA1L3 antibody (AB113657)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIPA1L3 antibody (AB113657)

Immunohistochemistry analysis of formalin-Fixed, paraffin-embedded Human brain cerebellum with ab113657 at 5 µg/ml.

Western blot - Anti-SIPA1L3 antibody (AB113657)
  • WB

Unknown

Western blot - Anti-SIPA1L3 antibody (AB113657)

All lanes:

Western blot - Anti-SIPA1L3 antibody (ab113657) at 1 µg/mL

All lanes:

SK-N-SH lysate

Predicted band size: 195 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SIPA1L3 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O60292

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIPA1L3 also known as Signal-Induced Proliferation-Associated 1 Like 3 or SPAR3 is a protein with a molecular mass of approximately 234 kDa. Mechanically SIPA1L3 functions as a Rap GTPase-activating protein (GAP) that regulates the activity of small G proteins by promoting the conversion of their active GTP-bound state to an inactive GDP-bound state. SIPA1L3 is widely expressed in several tissues with notable expression in the brain lens and retina indicating its involvement in neuronal development and visual processes.
Biological function summary

This protein is vital for signal transduction involved in cellular adhesion and cytoskeleton organization interacting with actin filaments to regulate cell shape and movement. SIPA1L3 is part of a multiprotein complex that influences intercellular junction formation contributing to the maintenance of cellular contacts in epithelial tissues. Its regulatory role in cellular dynamics suggests an impact on processes such as migration and proliferation.

Pathways

SIPA1L3 engages in the regulation of the Ras signaling pathway and the Rap1 signaling pathway which are important for cell growth survival and differentiation. Through its activity SIPA1L3 modulates interactions with related proteins such as Ras further influencing downstream signaling cascades. The interplay with these pathways positions SIPA1L3 as an important regulator of cellular responses to extracellular signals.

Altered expression or function of SIPA1L3 has been linked to conditions such as cataracts and neurological disorders. The misregulation of this protein can disrupt normal cellular adhesion and cytoskeleton dynamics leading to disease development. Additionally studies suggest that interactions with proteins in the Ras pathway may further contribute to its role in these diseases highlighting the relevance of SIPA1L3 in pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a critical role in epithelial cell morphogenesis, polarity, adhesion and cytoskeletal organization in the lens (PubMed : 26231217).
See full target information SIPA1L3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Development (Cambridge, England) 144:321-333 PubMed27993984

2016

An Epha4/Sipa1l3/Wnt pathway regulates eye development and lens maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie Rothe,Noreen Kanwal,Petra Dietmann,Franziska A Seigfried,Annemarie Hempel,Desiree Schütz,Dominik Reim,Rebecca Engels,Alexander Linnemann,Michael J Schmeisser,Juergen Bockmann,Michael Kühl,Tobias M Boeckers,Susanne J Kühl
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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