AB53721

Anti-SIRP alpha antibody

Name: Anti-SIRP alpha antibody (ab53721) | Abcam
Brand: Abcam Limited
SKU: AB53721
Price: 525 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(9 Publications)

Rabbit Polyclonal SIRP alpha antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human SIRPA aa 450-500.

View Alternative Names

CD172a, BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP, SIRPA, Tyrosine-protein phosphatase non-receptor type substrate 1, SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, p84, Bit, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody (AB53721)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody (AB53721)

ab53721 at 1/50 dilution, staining paraffin embedded human brain, in the presence (right) and absence (left) of blocking peptide.

Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody (AB53721)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody (AB53721)

ICC/IF image of ab53721 stained Mcf7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53721, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-SIRP alpha antibody (AB53721)

All lanes:

Western blot - Anti-SIRP alpha antibody (ab53721) at 1/500 dilution

Lane 1:

Extracts from HepG2 cells

Lane 2:

Extracts from HepG2 cells with immunizing peptide

Predicted band size: 55 kDa

Observed band size: 56 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human SIRPA aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

P78324

Specificity

Detects endogenous levels of total Sirp alpha1 protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIRP alpha also known as CD172a is a transmembrane receptor protein with a mass ranging between 70-110 kDa due to glycosylation. This protein extensively expresses on the surface of myeloid cells neurons and a subset of T-cells and is part of the immunoglobulin superfamily. SIRP alpha interacts with its ligand CD47 a widely expressed glycoprotein involved in immune response regulation. Its mechanical action primarily involves signal regulation through the recruitment of SHP-1 and SHP-2 two cytoplasmic tyrosine phosphatases.

Biological function summary

SIRP alpha functions significantly in the regulation of phagocytosis acting as a "don't eat me" signal to macrophages upon binding with CD47. It does not act alone; rather it is part of a complex that recruits SHP-1 and SHP-2 leading to inhibition of dephosphorylation activities essential for engulfment processes. This regulatory mechanism is important for maintaining cellular homeostasis ensuring that healthy cells are not mistakenly destroyed by the immune system.

Pathways

SIRP alpha plays an important role in the innate immune pathways involving the regulation of phagocytosis and cell-cell adhesion. Particularly it fits into the immune checkpoint pathways where it interacts closely with proteins like CD47 and plays a role in the interaction between the immune system and cancer cells. Through these pathways SIRP alpha helps maintain balance in the immune response allowing for the recognition of self versus non-self therefore preventing autoimmunity while facilitating the clearance of pathogens.

SIRP alpha is implicated in cancer and autoimmune diseases. In cancer its interaction with CD47 allows tumor cells to escape phagocytosis promoting tumor survival and growth. In autoimmune disorders dysregulated SIRP alpha expression or signaling could miscommunicate immune signals leading to the destruction of healthy tissue. Understanding the link between SIRP alpha and these conditions can reveal potential targets for therapeutic development especially using inhibitors or modulators targeting the SIRP alpha-CD47 interaction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Plays a role in antiviral immunity and limits new world arenavirus infection by decreasing virus internalization (By similarity). Receptor for THBS1 (PubMed : 24511121). Interaction with THBS1 stimulates phosphorylation of SIRPA (By similarity). In response to THBS1, involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production (PubMed : 24511121).

See full target information SIRPA

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 9:180 PubMed37291116

2023

SIRPα blockade improves the antitumor immunity of radiotherapy in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ji,Yuhan Zhang,Shengpeng Jiang,Lin Sun,Baozhong Zhang,Dongzhi Hu,Jun Wang,Lujun Zhao,Ping Wang,Zhen Tao

Cancers 15: PubMed37190208

2023

Immune Profile of Exosomes in African American Breast Cancer Patients Is Mediated by Kaiso/THBS1/CD47 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Md Shakir Uddin Ahmed,Brittany D Lord,Benjamin Adu Addai,Sandeep K Singhal,Kevin Gardner,Ahmad Bin Salam,Anghesom Ghebremedhin,Jason White,Iqbal Mahmud,Rachel Martini,Deepa Bedi,Huixian Lin,Jacqueline D Jones,Balasubramanyanam Karanam,Windy Dean-Colomb,William Grizzle,Honghe Wang,Melissa Davis,Clayton C Yates

Cancer immunology, immunotherapy : CII 72:1779-1788 PubMed36646952

2023

Prognostic role of the innate immune signature CD163 and "eat me" signal calreticulin in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tadatsugu Anno,Nobuyuki Tanaka,Kimiharu Takamatsu,Kyohei Hakozaki,Ryohei Kufukihara,Yuto Baba,Toshikazu Takeda,Kazuhiro Matsumoto,Shinya Morita,Takeo Kosaka,Shuji Mikami,Hiroshi Nishihara,Ryuichi Mizuno,Mototsugu Oya

Cancers 12: PubMed32645996

2020

Unique Spatial Immune Profiling in Pancreatic Ductal Adenocarcinoma with Enrichment of Exhausted and Senescent T Cells and Diffused CD47-SIRPα Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandros Papalampros,Michail Vailas,Konstantinos Ntostoglou,Maria Lopez Chiloeches,Stratigoula Sakellariou,Niki V Chouliari,Menelaos G Samaras,Paraskevi D Veltsista,Sofia D P Theodorou,Aggelos T Margetis,Anna Bergonzini,Lysandros Karydakis,Natasha Hasemaki,Sophia Havaki,Ioannis I Moustakas,Antonios Chatzigeorgiou,Timokratis Karamitros,Eleni Patsea,Christos Kittas,Andreas C Lazaris,Evangelos Felekouras,Vassilis G Gorgoulis,Teresa Frisan,Ioannis S Pateras

Frontiers in molecular neuroscience 11:295 PubMed30210289

2018

Cannabinoid 1 Receptor Signaling on Hippocampal GABAergic Neurons Influences Microglial Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Frank Ativie,Joanna A Komorowska,Eva Beins,Önder Albayram,Till Zimmer,Andreas Zimmer,Dario Tejera,Michael Heneka,Andras Bilkei-Gorzo

Cancer science 109:1300-1308 PubMed29473266

2018

Anti-human SIRPα antibody is a new tool for cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yoji Murata,Daisuke Tanaka,Daisuke Hazama,Tadahiko Yanagita,Yasuyuki Saito,Takenori Kotani,Per-Arne Oldenborg,Takashi Matozaki

JCI insight 2:e89140 PubMed28097229

2017

Anti-SIRP antibodies as a potential new tool for cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Tadahiko Yanagita,Yoji Murata,Daisuke Tanaka,Sei-Ichiro Motegi,Eri Arai,Edwin Widyanto Daniwijaya,Daisuke Hazama,Ken Washio,Yasuyuki Saito,Takenori Kotani,Hiroshi Ohnishi,Per-Arne Oldenborg,Noel Verjan Garcia,Masayuki Miyasaka,Osamu Ishikawa,Yae Kanai,Takahide Komori,Takashi Matozaki

Journal of immunology (Baltimore, Md. : 1950) 188:4496-505 PubMed22490440

2012

SIRPα/CD172a and FHOD1 are unique markers of littoral cells, a recently evolved major cell population of red pulp of human spleen.

Applications

Unspecified application

Species

Unspecified reactive species

Javier Gordon Ogembo,Danny A Milner,Keith G Mansfield,Scott J Rodig,George F Murphy,Jeffery L Kutok,Geraldine S Pinkus,Joyce D Fingeroth

Journal of immunology (Baltimore, Md. : 1950) 185:2998-3006 PubMed20675593

2010

Escherichia coli K1 promotes the ligation of CD47 with thrombospondin-1 to prevent the maturation of dendritic cells in the pathogenesis of neonatal meningitis.

Applications

Species

Human

Rahul Mittal,Ignacio Gonzalez-Gomez,Nemani V Prasadarao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SIRP alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody (AB53721)

Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody (AB53721)

Western blot - Anti-SIRP alpha antibody (AB53721)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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