AB8120

Anti-SIRP alpha antibody

Name: Anti-SIRP alpha antibody (ab8120) | Abcam
Brand: Abcam Limited
SKU: AB8120
Price: 535 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(14 Publications)

Rabbit Polyclonal SIRP alpha antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human SIRPA aa 450 to C-terminus.

View Alternative Names

CD172a, BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP, SIRPA, Tyrosine-protein phosphatase non-receptor type substrate 1, SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, p84, Bit, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3

2 Images

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Western blot - Anti-SIRP alpha antibody (AB8120)

All lanes:

Western blot - Anti-SIRP alpha antibody (ab8120) at 1/2000 dilution

All lanes:

THP-1 whole cell lysate

Predicted band size: 55 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody (AB8120)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody (AB8120)

ab8120 at 1μg/ml staining SIRP alpha in THP-1 cells by ICC/IF

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human SIRPA aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P78324

Specificity

Recognizes SIRPa1, 2 and 3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIRP alpha also known as CD172a is a transmembrane receptor protein with a mass ranging between 70-110 kDa due to glycosylation. This protein extensively expresses on the surface of myeloid cells neurons and a subset of T-cells and is part of the immunoglobulin superfamily. SIRP alpha interacts with its ligand CD47 a widely expressed glycoprotein involved in immune response regulation. Its mechanical action primarily involves signal regulation through the recruitment of SHP-1 and SHP-2 two cytoplasmic tyrosine phosphatases.

Biological function summary

SIRP alpha functions significantly in the regulation of phagocytosis acting as a "don't eat me" signal to macrophages upon binding with CD47. It does not act alone; rather it is part of a complex that recruits SHP-1 and SHP-2 leading to inhibition of dephosphorylation activities essential for engulfment processes. This regulatory mechanism is important for maintaining cellular homeostasis ensuring that healthy cells are not mistakenly destroyed by the immune system.

Pathways

SIRP alpha plays an important role in the innate immune pathways involving the regulation of phagocytosis and cell-cell adhesion. Particularly it fits into the immune checkpoint pathways where it interacts closely with proteins like CD47 and plays a role in the interaction between the immune system and cancer cells. Through these pathways SIRP alpha helps maintain balance in the immune response allowing for the recognition of self versus non-self therefore preventing autoimmunity while facilitating the clearance of pathogens.

SIRP alpha is implicated in cancer and autoimmune diseases. In cancer its interaction with CD47 allows tumor cells to escape phagocytosis promoting tumor survival and growth. In autoimmune disorders dysregulated SIRP alpha expression or signaling could miscommunicate immune signals leading to the destruction of healthy tissue. Understanding the link between SIRP alpha and these conditions can reveal potential targets for therapeutic development especially using inhibitors or modulators targeting the SIRP alpha-CD47 interaction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Plays a role in antiviral immunity and limits new world arenavirus infection by decreasing virus internalization (By similarity). Receptor for THBS1 (PubMed : 24511121). Interaction with THBS1 stimulates phosphorylation of SIRPA (By similarity). In response to THBS1, involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production (PubMed : 24511121).

See full target information SIRPA

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Esophagus : official journal of the Japan Esophageal Society : PubMed40926176

2025

Expression of cluster of differentiation 47 (CD47) and signal regulatory protein alpha (SIRPα) as prognostic biomarkers and potentially therapeutic targets in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Junpeng Li,Yohei Ozawa,Takeru Mozumi,Kaiyuan Jiang,Yusuke Taniyama,Chiaki Sato,Hiroshi Okamoto,Hirotaka Ishida,Naoto Ujiie,Shinobu Ohnuma,Michiaki Unno,Takashi Kamei

Acta biochimica et biophysica Sinica 57:782-791 PubMed39552221

2024

SIRPα modulates the podocyte cytoskeleton through influencing the phosphorylation of FAK at tyrosine residue 597.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Xia,Yue Zhao,Jing Tian,Xue Yang,Yun Fan,Shihui Dong,Fan Yang,Mingchao Zhang,Caihong Zeng

Cell communication and signaling : CCS 22:173 PubMed38462636

2024

Discovery of a novel small molecule as CD47/SIRPα and PD-1/PD-L1 dual inhibitor for cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Shengzhe Jin,Hongfei Wang,Yang Li,Jingwen Yang,Beibei Li,Peishang Shi,Xiangrui Zhang,Xiaowen Zhou,Xiuman Zhou,Xiaoshuang Niu,Menghan Wu,Yahong Wu,Wenjie Zhai,Yuanming Qi,Yanfeng Gao,Wenshan Zhao

Cancer cell 40:1324-1340.e8 PubMed36332624

2022

Tumor-intrinsic SIRPA promotes sensitivity to checkpoint inhibition immunotherapy in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhicheng Zhou,Mei-Ju May Chen,Yikai Luo,Kamalika Mojumdar,Xin Peng,Hu Chen,Shweta V Kumar,Rehan Akbani,Yiling Lu,Han Liang

Frontiers in neuroscience 16:788675 PubMed35360151

2022

Anomalous Levels of CD47/Signal Regulatory Protein Alpha in the Hippocampus Lead to Excess Microglial Engulfment in Mouse Model of Perioperative Neurocognitive Disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Min Shui,Yi Sun,Dandan Lin,Ziyi Xue,Jianhui Liu,Anshi Wu,Changwei Wei

Nature communications 12:2030 PubMed33795678

2021

Loss of microglial SIRPα promotes synaptic pruning in preclinical models of neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Ding,Jin Wang,Miaoxin Huang,Zhangpeng Chen,Jing Liu,Qipeng Zhang,Chenyu Zhang,Yang Xiang,Ke Zen,Liang Li

Kidney diseases (Basel, Switzerland) 6:422-433 PubMed33313063

2020

Podocyte-Released Migrasomes in Urine Serve as an Indicator for Early Podocyte Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Shan Li,Weiwei Rong,Caihong Zeng,Xiaodong Zhu,Qilin Chen,Limin Li,Zhi-Hong Liu,Ke Zen

Journal of neuroinflammation 16:277 PubMed31883525

2019

Deletion of SIRPα (signal regulatory protein-α) promotes phagocytic clearance of myelin debris in Wallerian degeneration, axon regeneration, and recovery from nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Gerard Elberg,Sigal Liraz-Zaltsman,Fanny Reichert,Takashi Matozaki,Michael Tal,Shlomo Rotshenker

JCI insight 5: PubMed30888336

2019

Signal regulatory protein α protects podocytes through promoting autophagic activity.

Applications

Unspecified application

Species

Unspecified reactive species

Limin Li,Ying Liu,Shan Li,Rong Yang,Caihong Zeng,Weiwei Rong,Hongwei Liang,Mingchao Zhang,Xiaodong Zhu,Koby Kidder,Yuan Liu,Zhihong Liu,Ke Zen

Biology open 6:358-364 PubMed28167493

2017

Smad4 regulates growth plate matrix production and chondrocyte polarity.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda T Whitaker,Ellora Berthet,Andrea Cantu,Diana J Laird,Tamara Alliston

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SIRP alpha antibody

Western blot - Anti-SIRP alpha antibody (AB8120)

Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody (AB8120)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise