AB314072

Anti-SIRP alpha antibody [BLR049F] - BSA free

Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal SIRP alpha antibody. Suitable for ICC, IHC-P, WB, IP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SIRPA aa 1-400.

View Alternative Names

CD172a, BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP, SIRPA, Tyrosine-protein phosphatase non-receptor type substrate 1, SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, p84, Bit, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3

4 Images

Supplier Data

Immunocytochemistry - Anti-SIRP alpha antibody [BLR049F] - BSA free (AB314072)

Detection of human SIRP alpha by immunocytochemistry. Sample : FFPE section of Malme-3M cells. Antibody : Rabbit anti-SIRP alpha recombinant monoclonal antibody [BLR049F] (A700-049) used at 1 : 250. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody [BLR049F] - BSA free (AB314072)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody [BLR049F] - BSA free (AB314072)

Detection of human SIRP alpha by immunohistochemistry. Sample : FFPE section of human breast carcinoma. Antibody : Rabbit anti-SIRP alpha recombinant monoclonal antibody [BLR049F] (A700-049) used at 1 : 250. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Immunoprecipitation - Anti-SIRP alpha antibody [BLR049F] - BSA free (AB314072)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-MEL-28 cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-SIRP alpha recombinant monoclonal antibody [BLR049F] (A700-049) used for IP at 20 µl per reaction. SIRP alpha was also immunoprecipitated by rabbit anti-SIRP alpha recombinant monoclonal antibody [BL-565-1D6]. For blotting immunoprecipitated SIRP alpha, A700-049 was used at 1 : 1000. Chemiluminescence with an exposure time of 30 seconds.

false

Supplier Data

Western blot - Anti-SIRP alpha antibody [BLR049F] - BSA free (AB314072)

Samples : Whole cell lysate (50 µg) from Malme-3M, HeLa, 786-O, HEK293T, U2OS, OVCAR-4, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-SIRP alpha recombinant monoclonal antibody [BLR049F] (A700-049 lot 1) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel : Rabbit antiActin (A700-057).

All lanes:

Western blot - Anti-SIRP alpha antibody [BLR049F] - BSA free (ab314072) at 1/1000 dilution

Lane 1:

Malme-3M Whole cell lysate at 50 µg

Lane 2:

HeLa Whole cell lysate at 50 µg

Lane 3:

786-O Whole cell lysate at 50 µg

Lane 4:

HEK293T Whole cell lysate at 50 µg

Lane 5:

U2OS Whole cell lysate at 50 µg

Lane 6:

OVCAR-4 Whole cell lysate at 50 µg

Lane 7:

SK-MEL-28 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR049F

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, IP, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SIRPA aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

P78324

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

style

left-column

Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIRP alpha also known as CD172a is a transmembrane receptor protein with a mass ranging between 70-110 kDa due to glycosylation. This protein extensively expresses on the surface of myeloid cells neurons and a subset of T-cells and is part of the immunoglobulin superfamily. SIRP alpha interacts with its ligand CD47 a widely expressed glycoprotein involved in immune response regulation. Its mechanical action primarily involves signal regulation through the recruitment of SHP-1 and SHP-2 two cytoplasmic tyrosine phosphatases.

Biological function summary

SIRP alpha functions significantly in the regulation of phagocytosis acting as a "don't eat me" signal to macrophages upon binding with CD47. It does not act alone; rather it is part of a complex that recruits SHP-1 and SHP-2 leading to inhibition of dephosphorylation activities essential for engulfment processes. This regulatory mechanism is important for maintaining cellular homeostasis ensuring that healthy cells are not mistakenly destroyed by the immune system.

Pathways

SIRP alpha plays an important role in the innate immune pathways involving the regulation of phagocytosis and cell-cell adhesion. Particularly it fits into the immune checkpoint pathways where it interacts closely with proteins like CD47 and plays a role in the interaction between the immune system and cancer cells. Through these pathways SIRP alpha helps maintain balance in the immune response allowing for the recognition of self versus non-self therefore preventing autoimmunity while facilitating the clearance of pathogens.

SIRP alpha is implicated in cancer and autoimmune diseases. In cancer its interaction with CD47 allows tumor cells to escape phagocytosis promoting tumor survival and growth. In autoimmune disorders dysregulated SIRP alpha expression or signaling could miscommunicate immune signals leading to the destruction of healthy tissue. Understanding the link between SIRP alpha and these conditions can reveal potential targets for therapeutic development especially using inhibitors or modulators targeting the SIRP alpha-CD47 interaction.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Plays a role in antiviral immunity and limits new world arenavirus infection by decreasing virus internalization (By similarity). Receptor for THBS1 (PubMed : 24511121). Interaction with THBS1 stimulates phosphorylation of SIRPA (By similarity). In response to THBS1, involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production (PubMed : 24511121).

See full target information SIRPA

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SIRP alpha antibody [BLR049F] - BSA free

Immunocytochemistry - Anti-SIRP alpha antibody [BLR049F] - BSA free (AB314072)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody [BLR049F] - BSA free (AB314072)

Immunoprecipitation - Anti-SIRP alpha antibody [BLR049F] - BSA free (AB314072)

Western blot - Anti-SIRP alpha antibody [BLR049F] - BSA free (AB314072)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise