Mouse Monoclonal SIRP alpha antibody. Suitable for IP, Flow Cyt, WB, IHC-Fr and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Sirpa protein.
IgG1
Mouse
Preservative: 0.09% Sodium azide
Liquid
Monoclonal
| IP | Flow Cyt | WB | IHC-Fr | |
|---|---|---|---|---|
| Rat | Expected | Expected | Expected | Expected |
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
Tyrosine-protein phosphatase non-receptor type substrate 1, SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, Macrophage membrane protein MFP150, Signal-regulatory protein alpha-1, Bit, Sirp-alpha-1, Sirpa, Bit, Sirp, Shps1, Ptpns1, Mfr
Mouse Monoclonal SIRP alpha antibody. Suitable for IP, Flow Cyt, WB, IHC-Fr and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Sirpa protein.
IgG1
Mouse
Preservative: 0.09% Sodium azide
Liquid
Monoclonal
ED9
Affinity purification Protein A
This antibody was purified from tissue culture supernatant.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Clone ED9 has been reported to block the interaction of CD172a - CD47. Removal of Sodium Azide is recommended prior to use in functional assays.
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This supplementary information is collated from multiple sources and compiled automatically.
SIRP alpha also known as CD172a is a transmembrane receptor protein with a mass ranging between 70-110 kDa due to glycosylation. This protein extensively expresses on the surface of myeloid cells neurons and a subset of T-cells and is part of the immunoglobulin superfamily. SIRP alpha interacts with its ligand CD47 a widely expressed glycoprotein involved in immune response regulation. Its mechanical action primarily involves signal regulation through the recruitment of SHP-1 and SHP-2 two cytoplasmic tyrosine phosphatases.
SIRP alpha functions significantly in the regulation of phagocytosis acting as a "don't eat me" signal to macrophages upon binding with CD47. It does not act alone; rather it is part of a complex that recruits SHP-1 and SHP-2 leading to inhibition of dephosphorylation activities essential for engulfment processes. This regulatory mechanism is important for maintaining cellular homeostasis ensuring that healthy cells are not mistakenly destroyed by the immune system.
SIRP alpha plays an important role in the innate immune pathways involving the regulation of phagocytosis and cell-cell adhesion. Particularly it fits into the immune checkpoint pathways where it interacts closely with proteins like CD47 and plays a role in the interaction between the immune system and cancer cells. Through these pathways SIRP alpha helps maintain balance in the immune response allowing for the recognition of self versus non-self therefore preventing autoimmunity while facilitating the clearance of pathogens.
SIRP alpha is implicated in cancer and autoimmune diseases. In cancer its interaction with CD47 allows tumor cells to escape phagocytosis promoting tumor survival and growth. In autoimmune disorders dysregulated SIRP alpha expression or signaling could miscommunicate immune signals leading to the destruction of healthy tissue. Understanding the link between SIRP alpha and these conditions can reveal potential targets for therapeutic development especially using inhibitors or modulators targeting the SIRP alpha-CD47 interaction.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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