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AB234681

Anti-SIRPB1 antibody

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(2 Publications)

Rabbit Polyclonal SIRPB1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Signal-regulatory protein beta-1 aa 1 to C-terminus.

View Alternative Names

CD172b, Signal-regulatory protein beta-1, SIRP-beta-1, CD172 antigen-like family member B, SIRPB1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SIRPB1 antibody (AB234681)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SIRPB1 antibody (AB234681)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SIRPB1 (green) using ab234681 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-SIRPB1 antibody (AB234681)
  • WB

Supplier Data

Western blot - Anti-SIRPB1 antibody (AB234681)

All lanes:

Western blot - Anti-SIRPB1 antibody (ab234681) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma cell line) whole cell lysate

Lane 2:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

Lane 3:

A575 (human malignant melanoma cell line) whole cell lysate

Lane 4:

Mouse heart lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Signal-regulatory protein beta-1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00241

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Signal regulatory protein beta-1 (SIRPB1) also known as CD172b is a transmembrane glycoprotein that functions as an inhibitory receptor. It weighs about 55 kDa. SIRPB1 is mainly expressed on myeloid cells including monocytes macrophages and granulocytes. It contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) which recruits phosphatases such as Src homology region 2 domain-containing phosphatase-1 (SHP-1) upon phosphorylation. This motif plays an important role in mediating inhibitory signals inside the cell.
Biological function summary

Within the immune system context SIRPB1 acts to regulate functions related to myeloid cells. It does not form part of a larger receptor complex but significantly influences cell communication. It modulates responses like phagocytosis and cellular activation maintaining balanced immune reactions. The interaction with its ligands like CD47 or DAP12 provides insights into its ability to send inhibitory signals contributing to the overall control of immune responses.

Pathways

SIRPB1 gets involved in immune regulation and signal transduction. It plays a significant role in the CD47-SIRP signaling pathway which affects processes like cell migration and phagocytosis. The interaction with SHP-1 and SHP-2 phosphatases signifies its integration within signaling networks governing negative regulation of cellular activation. Through these pathways SIRPB1 interacts with related proteins like SIRPA and SIRPG which have similar functions yet distinct roles within the immune system.

SIRPB1 has associations with inflammatory conditions and cancer. In inflammatory disorders like rheumatoid arthritis altered expression of SIRPB1 can contribute to pathogenesis by modifying immune cell activation further linking it with SIRPA in the modulation of immune responses. In cancer SIRPB1's role extends to tumor immune evasion where upregulated CD47 affects SIRPB1 pathways to inhibit phagocytosis by macrophages influencing tumor progression. Understanding SIRPB1's connections with these proteins and pathways opens avenues for therapeutic interventions in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Also participates in the recruitment of tyrosine kinase SYK. Triggers activation of myeloid cells when associated with TYROBP (PubMed : 10604985).
See full target information Signal-regulatory protein beta-1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine : PubMed40399049

2025

Clinical Significance and Functional Analysis of miR-487b-3p in Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shixi Zhang,Jing Chen,Qian Gao,Yan Li,Chao Wang

Virus research 355:199570 PubMed40188851

2025

Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses.

Applications

Unspecified application

Species

Unspecified reactive species

Rupaly Akhter,Kazi Anowar Hossain,Bouchra Kitab,Yoshihiro Sakoda,Kyoko Tsukiyama-Kohara
View all publications
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Product promise

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