AB246522

Anti-SIRT3 antibody [EPR23514-36]

RabMAb
Recombinant
20ul selling size
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(15 Publications)

Rabbit Recombinant Monoclonal SIRT3 antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 15 publications.

View Alternative Names

Sir2l3, Sirt3, NAD-dependent protein deacetylase sirtuin-3, NAD-dependent protein delactylase sirtuin-3, Regulatory protein SIR2 homolog 3, SIR2-like protein 3, mSIR2L3

5 Images

Supplier Data

Western blot - Anti-SIRT3 antibody [EPR23514-36] (AB246522)

Blocking/diluting buffer and concentration : 5% NFDM/TBST

Negative control : Spleen (PMID : 25566066).

Exposure time : 15 secs.

All lanes:

Western blot - Anti-SIRT3 antibody [EPR23514-36] (ab246522) at 1/1000 dilution

Lane 1:

Rat liver tissue lysate 20 μg

Lane 2:

Rat kidney tissue lysate 20 μg

Lane 3:

Rat spleen tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 43 kDa

Observed band size: 29 kDa

false

Supplier Data

Western blot - Anti-SIRT3 antibody [EPR23514-36] (AB246522)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 3 secs.

All lanes:

Western blot - Anti-SIRT3 antibody [EPR23514-36] (ab246522) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 43 kDa

false

Supplier Data

Western blot - Anti-SIRT3 antibody [EPR23514-36] (AB246522)

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Spleen (PMID : 25566066).

Exposure time : 15 secs.

All lanes:

Western blot - Anti-SIRT3 antibody [EPR23514-36] (ab246522) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate 20 μg

Lane 2:

Mouse kidney tissue lysate 20 μg

Lane 3:

Mouse spleen tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 43 kDa

false

Supplier Data

Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] (AB246522)

SIRT3 was immunoprecipitated from 0.35 mg rat liver lysate with ab246522 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246522 at a 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat liver lysate 10μg
Lane 2 : ab246522 IP in rat liver lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab246522 in rat liver lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 secs.

The target band size is very close to IgG light chain.

All lanes:

Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] (ab246522)

Predicted band size: 43 kDa

false

Supplier Data

Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] (AB246522)

SIRT3 was immunoprecipitated from 0.35 mg mouse liver lysate with ab246522 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246522 at a 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse liver lysate 10μg
Lane 2 : ab246522 IP in mouse liver lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab246522 in mouse liver lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 secs.

The target band size is very close to IgG light chain.

All lanes:

Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] (ab246522)

Predicted band size: 43 kDa

false

Carrier free

Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23514-36

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIRT3 also known as Sirtuin 3 and mitochondrial sirtuin is a deacetylase with a mass of roughly 44 kDa. This protein is mostly found in the mitochondria where it regulates the acetylation state of various substrates. It plays an important role in maintaining mitochondrial homeostasis by modifying protein activity. SIRT3 is evolutionarily conserved and highly expressed in tissues with high energy demand like the heart liver and brown adipose tissue.

Biological function summary

SIRT3 modulates energy homeostasis by deacetylating and regulating enzymes involved in metabolism. It is a part of the mitochondrial sirtuin family contributing to fatty acid oxidation and the antioxidant defense system. By targeting key metabolic enzymes SIRT3 directly impacts the tricarboxylic acid (TCA) cycle and oxidative phosphorylation processes. These functions highlight its role in maintaining energy efficiency and reducing reactive oxidative species.

Pathways

SIRT3 participates in vital metabolic pathways such as the TCA cycle and the urea cycle. It interacts with other proteins like MnSOD and IDH2 to enhance mitochondrial function and overall cellular metabolism. This helps control the balance between energy production and consumption integrating signals from the AMPK and NAD+-dependent pathways to coordinate responses to changes in energy availability.

SIRT3 has implications in metabolic syndrome and cancer. Reduced SIRT3 activity is associated with increased susceptibility to metabolic disturbances like obesity and type 2 diabetes. Its deficiency affects the regulation of ROS and metabolic adaptation linking it to tumorigenesis and cancer progression. As it connects with proteins like HIF-1α in hypoxic conditions SIRT3 plays a role in cellular adaptation to stress influencing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

NAD-dependent protein deacetylase (PubMed : 17923681, PubMed : 18794531, PubMed : 21172655, PubMed : 23835326, PubMed : 26620563). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed : 17923681, PubMed : 18794531, PubMed : 21172655, PubMed : 23835326). Known targets include ACSS1, IDH, GDH, PDHA1, SOD2, LCAD, SDHA and the ATP synthase subunit ATP5PO (PubMed : 16790548, PubMed : 18794531, PubMed : 21172655). Contributes to the regulation of the cellular energy metabolism (PubMed : 23835326, PubMed : 36804859). Important for regulating tissue-specific ATP levels (PubMed : 18794531, PubMed : 24252090). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed : 23283301). Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (PubMed : 26620563). Regulates hepatic lipogenesis (PubMed : 36804859). Uses NAD(+) substrate imported by SLC25A47, triggering downstream activation of PRKAA1/AMPK-alpha signaling cascade that ultimately downregulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance (PubMed : 36804859). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by mediating delactylation of proteins, such as CCNE2 and 'Lys-16' of histone H4 (H4K16la) (By similarity).

See full target information Sirt3

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Translational cancer research 14:1311-1322 PubMed40104701

2025

Total Astragalus saponins promote ferroptosis in gastric cancer cells by upregulating SIRT3.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zou,Jingling Zhao,Chengyin Li,Rui Wang,Xiaocui Jiang,Zhongyi Zhu,Qiyuan Wang,Min Xiao

Journal of translational medicine 22:267 PubMed38468343

2024

Kynurenine in IDO1 cancer cell-derived extracellular vesicles promotes angiogenesis by inducing endothelial mitophagy in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Ying,Xiaocui Zheng,Xiaoqian Zhang,Yujia Yin,Xipeng Wang

Journal of translational medicine 22:26 PubMed38183100

2024

(Pro)renin receptor mediates tubular epithelial cell pyroptosis in diabetic kidney disease via DPP4-JNK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shiying Xie,Shicong Song,Sirui Liu,Qiong Li,Wei Zou,Jianting Ke,Cheng Wang

International journal of biological sciences 20:585-605 PubMed38169591

2024

SIRT5-related lysine demalonylation of GSTP1 contributes to cardiomyocyte pyroptosis suppression in diabetic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Can Wei,Meixin Shi,Shiyun Dong,Zhitao Li,Bingbing Zhao,Dan Liu,Guopeng Li,Jie Cen,Ligen Yu,Xiao Liang,Lili Shi

Scientific reports 13:22085 PubMed38086958

2023

Heavy-chain antibody targeting of CD38 NAD hydrolase ectoenzyme to prevent fibrosis in multiple organs.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Shi,Asif Amin,Pranjali Dalvi,Wenxia Wang,Nicholas Lukacs,Li Kai,Paul Cheresh,Thais R Peclat,Claudia C Chini,Eduardo N Chini,Wim van Schooten,John Varga

Scientific reports 13:21320 PubMed38044382

2023

Perampanel attenuates oxidative stress and pyroptosis following subarachnoid hemorrhage via the SIRT3/FOXO3α pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongqiao Yang,Changgeng Ding,Ming Cheng,Zhengwei Sheng,Lei Chen,Junhui Chen,Yuhai Wang

Frontiers in pharmacology 14:1124633 PubMed37251330

2023

PTPN2 targets TAK1 for dephosphorylation to improve cellular senescence and promote adipose tissue browning in T2DM.

Applications

Unspecified application

Species

Unspecified reactive species

Yapeng Liu,Lu Han,Ping Zhu,Ming Song,Yaoyuan Zhang,Linlin Meng,Wei Zhang,Cheng Zhang,Ming Zhong

Mediators of inflammation 2023:6638929 PubMed37057132

2023

ANXA1sp Protects against Sepsis-Induced Myocardial Injury by Inhibiting Ferroptosis-Induced Cardiomyocyte Death via SIRT3-Mediated p53 Deacetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Song Qin,Yingcong Ren,Banghai Feng,Xiaoqin Wang,Junya Liu,Jie Zheng,Kang Li,Miao Chen,Tao Chen,Hong Mei,Xiaoyun Fu

Nutrients 15: PubMed36678226

2023

Dihydromyricetin Protects Intestinal Barrier Integrity by Promoting IL-22 Expression in ILC3s through the AMPK/SIRT3/STAT3 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhou,Jing Yue,Yu Yao,Pengfei Hou,Ting Zhang,Qianyong Zhang,Long Yi,Mantian Mi

Oxidative medicine and cellular longevity 2022:9041914 PubMed36211825

2022

Downregulation of SIRT3 Aggravates Lung Ischemia Reperfusion Injury by Increasing Mitochondrial Fission and Oxidative Stress through HIF-1-Dependent Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Chunxia Liu,Shenglin Pei,Huijun Dai,Zhen Liu,Mengling Ye,Hao Liu,Xiaojing He,Siyi Wu,Yi Qin,Fei Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SIRT3 antibody [EPR23514-36]

Western blot - Anti-SIRT3 antibody [EPR23514-36] (AB246522)

Western blot - Anti-SIRT3 antibody [EPR23514-36] (AB246522)

Western blot - Anti-SIRT3 antibody [EPR23514-36] (AB246522)

Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] (AB246522)

Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] (AB246522)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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