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AB270523

Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SIRT3 antibody. Carrier free. Suitable for IP, WB and reacts with Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

Sir2l3, Sirt3, NAD-dependent protein deacetylase sirtuin-3, NAD-dependent protein delactylase sirtuin-3, Regulatory protein SIR2 homolog 3, SIR2-like protein 3, mSIR2L3

2 Images
Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (AB270523)
  • IP

Supplier Data

Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (AB270523)

SIRT3 was immunoprecipitated from 0.35 mg mouse liver lysate with ab246522 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246522 at a 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse liver lysate 10μg
Lane 2 : ab246522 IP in mouse liver lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab246522 in mouse liver lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 secs.

The target band size is very close to IgG light chain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246522).

All lanes:

Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] (<a href='/en-us/products/primary-antibodies/sirt3-antibody-epr23514-36-ab246522'>ab246522</a>)

Predicted band size: 43 kDa

false

Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (AB270523)
  • IP

Supplier Data

Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (AB270523)

SIRT3 was immunoprecipitated from 0.35 mg rat liver lysate with ab246522 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246522 at a 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat liver lysate 10μg
Lane 2 : ab246522 IP in rat liver lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab246522 in rat liver lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 secs.

The target band size is very close to IgG light chain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246522).

All lanes:

Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] (<a href='/en-us/products/primary-antibodies/sirt3-antibody-epr23514-36-ab246522'>ab246522</a>)

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23514-36

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

ab270523 is the carrier-free version of ab246522.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIRT3 also known as Sirtuin 3 and mitochondrial sirtuin is a deacetylase with a mass of roughly 44 kDa. This protein is mostly found in the mitochondria where it regulates the acetylation state of various substrates. It plays an important role in maintaining mitochondrial homeostasis by modifying protein activity. SIRT3 is evolutionarily conserved and highly expressed in tissues with high energy demand like the heart liver and brown adipose tissue.
Biological function summary

SIRT3 modulates energy homeostasis by deacetylating and regulating enzymes involved in metabolism. It is a part of the mitochondrial sirtuin family contributing to fatty acid oxidation and the antioxidant defense system. By targeting key metabolic enzymes SIRT3 directly impacts the tricarboxylic acid (TCA) cycle and oxidative phosphorylation processes. These functions highlight its role in maintaining energy efficiency and reducing reactive oxidative species.

Pathways

SIRT3 participates in vital metabolic pathways such as the TCA cycle and the urea cycle. It interacts with other proteins like MnSOD and IDH2 to enhance mitochondrial function and overall cellular metabolism. This helps control the balance between energy production and consumption integrating signals from the AMPK and NAD+-dependent pathways to coordinate responses to changes in energy availability.

SIRT3 has implications in metabolic syndrome and cancer. Reduced SIRT3 activity is associated with increased susceptibility to metabolic disturbances like obesity and type 2 diabetes. Its deficiency affects the regulation of ROS and metabolic adaptation linking it to tumorigenesis and cancer progression. As it connects with proteins like HIF-1α in hypoxic conditions SIRT3 plays a role in cellular adaptation to stress influencing disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

NAD-dependent protein deacetylase (PubMed : 17923681, PubMed : 18794531, PubMed : 21172655, PubMed : 23835326, PubMed : 26620563). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed : 17923681, PubMed : 18794531, PubMed : 21172655, PubMed : 23835326). Known targets include ACSS1, IDH, GDH, PDHA1, SOD2, LCAD, SDHA and the ATP synthase subunit ATP5PO (PubMed : 16790548, PubMed : 18794531, PubMed : 21172655). Contributes to the regulation of the cellular energy metabolism (PubMed : 23835326, PubMed : 36804859). Important for regulating tissue-specific ATP levels (PubMed : 18794531, PubMed : 24252090). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed : 23283301). Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (PubMed : 26620563). Regulates hepatic lipogenesis (PubMed : 36804859). Uses NAD(+) substrate imported by SLC25A47, triggering downstream activation of PRKAA1/AMPK-alpha signaling cascade that ultimately downregulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance (PubMed : 36804859). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by mediating delactylation of proteins, such as CCNE2 and 'Lys-16' of histone H4 (H4K16la) (By similarity).
See full target information Sirt3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 9: PubMed33802221

2021

Asiatic Acid Prevents Cognitive Deficits by Inhibiting Calpain Activation and Preserving Synaptic and Mitochondrial Function in Rats with Kainic Acid-Induced Seizure.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Wei Lu,Tzu-Yu Lin,Tai-Long Pan,Pei-Wen Wang,Kuan-Ming Chiu,Ming-Yi Lee,Su-Jane Wang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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