Goat Polyclonal SIRT4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human SIRT4 aa 300 to C-terminus.
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- Cells 11:2022Autophagy in Rat Müller Glial Cells Is Modulated by the Sirtuin 4/AMPK/mTOR Pathway and Induces Apoptosis under Oxidative Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengqi Qin,Zhi Xie,Ting Cao,Zhiruo Wang,Xiaoyu Zhang,Feifei Wang,Wei Wei,Ming Jin,Jingyuan Ma,Ling Zeng,Yanan Wang,Shaonan Pei,Xu ZhangPubMed 36078054
- Frontiers in oncology 12:8724442022SIRT4-Catalyzed Deacetylation of Axin1 Modulates the Wnt/β-Catenin Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuting Wang,Jicheng Yue,Mingzhe Xiao,Xiaomei Lu,Yuen Eugene ChinPubMed 35707358
- Frontiers in neuroscience 16:8404432022SIRT4 Is Highly Expressed in Retinal Müller Glial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wei,Piaopiao Hu,Mengqi Qin,Guiping Chen,Feifei Wang,Shengrui Yao,Ming Jin,Zhi Xie,Xu ZhangPubMed 35185463
- Molecular medicine reports 24:2021Circular RNA OMA1 regulates the progression of breast cancer via modulation of the miR‑1276/SIRT4 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingli Xu,Ke Xu,Lijun Xiang,Jiamei YanPubMed 34414449
- Theranostics 10:9458-94762020Loss of SIRT4 promotes the self-renewal of Breast Cancer Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLutao Du et. al.PubMed 32863939
- Molecular cell 75:823-834.e52019SENP1-Sirt3 Signaling Controls Mitochondrial Protein Acetylation and Metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianshi Wang,Ying Cao,Quan Zheng,Jun Tu,Wei Zhou,Jianli He,Jie Zhong,Yalan Chen,Jiqiu Wang,Rong Cai,Yong Zuo,Bo Wei,Qiuju Fan,Jie Yang,Yicheng Wu,Jing Yi,Dali Li,Mingyao Liu,Chuangui Wang,Aiwu Zhou,Yu Li,Xuefeng Wu,Wen Yang,Y Eugene Chin,Guoqiang Chen,Jinke ChengPubMed 31302001
- International journal of molecular medicine 44:157-1712019Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojing Fan et. al.PubMed 31017270
- Bioscience reports 38:2018Study of expression analysis of and the coordinate regulation of bovine adipocyte differentiation by and its transcription factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJieyun Hong et. al.PubMed 30442871
- Neuroscience 393:196-2052018The Stress c-Jun N-terminal Kinase Signaling Pathway Activation Correlates with Synaptic Pathology and Presents A Sex Bias in P301L Mouse Model of Tauopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLucia Buccarello et. al.PubMed 30315879
- Oncology letters 16:1675-16812018Knockout of SIRT4 decreases chemosensitivity to 5-FU in colorectal cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanyuan Zhu et. al.PubMed 30008852
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SIRT4 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6E7
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Dog, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-3.00000 µg/mL | Notes Steamed antigen retrieval with citrate buffer pH 6 is recommended. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Dog, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Acts as a NAD-dependent protein lipoamidase, biotinylase, deacetylase and ADP-ribosyl transferase (PubMed:16959573, PubMed:17715127, PubMed:24052263, PubMed:25525879). Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications (PubMed:24052263, PubMed:25525879). Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed:25525879). Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity (PubMed:16959573, PubMed:17715127). Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed:16959573, PubMed:17715127). In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation (PubMed:23663782). Acts as a tumor suppressor (PubMed:23562301, PubMed:23663782). Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (By similarity). Does not seem to deacetylate PC (PubMed:23438705). Controls fatty acid oxidation by inhibiting PPARA transcriptional activation (PubMed:24043310). Impairs SIRT1-PPARA interaction probably through the regulation of NAD(+) levels (PubMed:24043310). Down-regulates insulin secretion (PubMed:17715127).
Alternative names
SIR2L4, SIRT4, NAD-dependent ADP-ribosyltransferase sirtuin-4, NAD-dependent protein biotinylase sirtuin-4, NAD-dependent protein deacetylase sirtuin-4, Regulatory protein SIR2 homolog 4, SIR2-like protein 4
Recommended products
Goat Polyclonal SIRT4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human SIRT4 aa 300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SIRT4 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6E7
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SIRT4 also known as Sirtuin 4 is a mitochondrial protein with a mass of approximately 36 kDa. It belongs to the sirtuin family of NAD+-dependent deacetylases. Unlike many other sirtuins SIRT4 does not function as a deacetylase; instead it exhibits ADP-ribosyltransferase activity. This activity regulates a range of mitochondrial functions. SIRT4 is expressed in various tissues including the liver pancreas and muscles where it plays different roles based on the tissue type.
- Biological function summary
SIRT4 regulates energy metabolism and cellular stress responses. It plays key roles in the regulation of mitochondrial functions during fasting and impacts gluconeogenesis and fatty acid oxidation. SIRT4 inhibits glutamate dehydrogenase (GDH) affecting amino acid metabolism and insulin secretion. By acting in this capacity SIRT4 controls the flow of nutrients and energy homeostasis exhibiting its critical role in metabolic regulation.
- Pathways
SIRT4 integrates into a broader metabolic network including the insulin signaling and AMPK pathways. It interacts with proteins like GDH and AMPK influencing mitochondrial energy output and stress response. This relationship places SIRT4 in an important position for maintaining energy balance and responding to cellular metabolic demands.
- Associated diseases and disorders
SIRT4 links to metabolic conditions such as diabetes and metabolic syndrome. Dysregulation of SIRT4 activity can lead to impaired insulin secretion and glucose intolerance. Furthermore SIRT4's interaction with other sirtuins particularly SIRT1 suggests a functional interplay in protecting against metabolic stress and possibly influencing the development of these metabolic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-SIRT4 antibody (ab10140)
ab10140 staining (1μg/ml) of Human Kidney lysate (RIPA buffer, 35μg total protein per lane).Primary incubated for 1 hour.Detected by western blot using chemiluminescence.
Incubation with the primary Ab was carried out for 1 hr at room temperature.
All lanes: Western blot - Anti-SIRT4 antibody (ab10140) at 1 µg/mL
All lanes: Human Kidney Lysate (RIPA buffer).35 ug total protein was loaded.
Predicted band size: 35 kDa
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