AB124521

Anti-SIRT4 antibody

Be the first to review this product! Submit a review

(14 Publications)

Rabbit Polyclonal Sirt4 antibody. Suitable for WB and reacts with Rat samples. Cited in 14 publications.

1 Images

Unknown

Western blot - Anti-SIRT4 antibody (AB124521)

All lanes:

Western blot - Anti-SIRT4 antibody (ab124521) at 4 µg/mL

All lanes:

Rat kidney tissue lysate

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-4 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

This product is manufactured by BioVision, an Abcam company and was previously called 3224 SIRT4 Antibody. 3224-100 is the same size as the 100 µg size of ab124521.

The antibody solution should be gently mixed before use.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.15% EDTA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIRT4 also known as Sirtuin 4 is a mitochondrial protein with a mass of approximately 36 kDa. It belongs to the sirtuin family of NAD+-dependent deacetylases. Unlike many other sirtuins SIRT4 does not function as a deacetylase; instead it exhibits ADP-ribosyltransferase activity. This activity regulates a range of mitochondrial functions. SIRT4 is expressed in various tissues including the liver pancreas and muscles where it plays different roles based on the tissue type.

Biological function summary

SIRT4 regulates energy metabolism and cellular stress responses. It plays key roles in the regulation of mitochondrial functions during fasting and impacts gluconeogenesis and fatty acid oxidation. SIRT4 inhibits glutamate dehydrogenase (GDH) affecting amino acid metabolism and insulin secretion. By acting in this capacity SIRT4 controls the flow of nutrients and energy homeostasis exhibiting its critical role in metabolic regulation.

Pathways

SIRT4 integrates into a broader metabolic network including the insulin signaling and AMPK pathways. It interacts with proteins like GDH and AMPK influencing mitochondrial energy output and stress response. This relationship places SIRT4 in an important position for maintaining energy balance and responding to cellular metabolic demands.

SIRT4 links to metabolic conditions such as diabetes and metabolic syndrome. Dysregulation of SIRT4 activity can lead to impaired insulin secretion and glucose intolerance. Furthermore SIRT4's interaction with other sirtuins particularly SIRT1 suggests a functional interplay in protecting against metabolic stress and possibly influencing the development of these metabolic disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information Sirt4

style

left-column

Publications (14)

Recent publications for all applications. Explore the full list and refine your search

International heart journal 63:602-611 PubMed35650160

2022

Downregulation of lncRNA MALAT1 Inhibits Angiotensin II-induced Hypertrophic Effects of Cardiomyocytes by Regulating SIRT4 via miR-93-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Huanchun Ji,Jingxian Qu,Wei Peng,Long Yang

Phytotherapy research : PTR 36:2940-2951 PubMed35537702

2022

Trilobatin promotes angiogenesis after cerebral ischemia-reperfusion injury via SIRT7/VEGFA signaling pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Fengying Huang,Lingyu Luo,Yujia Wu,Dianya Xia,Fan Xu,Jianmei Gao,Jingshan Shi,Qihai Gong

Frontiers in neuroscience 16:840443 PubMed35185463

2022

SIRT4 Is Highly Expressed in Retinal Müller Glial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wei,Piaopiao Hu,Mengqi Qin,Guiping Chen,Feifei Wang,Shengrui Yao,Ming Jin,Zhi Xie,Xu Zhang

International journal of obesity (2005) 46:969-976 PubMed35058572

2022

LncRNA DLEU2 regulates sirtuins and mitochondrial respiratory chain complex IV: a novel pathway in obesity and offspring's health.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhang,Matthew Krivacka Kay,Min Hi Park,Sunitha Meruvu,Catherine Powell,Mahua Choudhury

Evidence-based complementary and alternative medicine : eCAM 2022:6730559 PubMed35154350

2022

Paeoniflorin Enhances the Sensitivity of ER-Positive Breast Cancer Cells to Tamoxifen through Promoting Sirtuin 4.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Zhang,Nan Wu,Zhi-Jun Song,Zheng-Fu Tai

Mediators of inflammation 2021:6639252 PubMed33927570

2021

Toll-Like Receptor 4 Mediated Oxidized Low-Density Lipoprotein-Induced Foam Cell Formation in Vascular Smooth Muscle Cells via Src and Sirt1/3 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongli Chen,Qiqi Xue,Lijuan Cao,Yanpin Wang,Yuanyuan Chen,Xiaojie Zhang,Fan Xiao,Ying Yang,Melvin R Hayden,Yan Liu,Ke Yang

FEBS open bio 11:921-931 PubMed33455080

2021

Dysregulation of the Sirt5/IDH2 axis contributes to sunitinib resistance in human renal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Meng,Deqiang Chen,Gaopei Meng,Li Lu,Chenggang Han

Redox biology 40:101843 PubMed33385630

2021

HIV TAT-mediated microglial senescence: Role of SIRT3-dependent mitochondrial oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Annadurai Thangaraj,Ernest T Chivero,Ashutosh Tripathi,Seema Singh,Fang Niu,Ming-Lei Guo,Prakash Pillai,Palsamy Periyasamy,Shilpa Buch

Immunology 158:362-374 PubMed31559637

2019

Sirtuin4 suppresses the anti-neuroinflammatory activity of infiltrating regulatory T cells in the traumatically injured spinal cord.

Applications

Unspecified application

Species

Unspecified reactive species

Wenping Lin,Wenkai Chen,Weifeng Liu,Zhengquan Xu,Liqun Zhang

Molecular medicine reports 19:4973-4979 PubMed31059091

2019

SIRT4 suppresses the PI3K/Akt/NF‑κB signaling pathway and attenuates HUVEC injury induced by oxLDL.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Tao,Songping Yu,Min Chao,Yang Wang,Jianhua Xiong,Hengli Lai

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SIRT4 antibody

Western blot - Anti-SIRT4 antibody (AB124521)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise