Anti-SIX1 antibody [1229]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SIX1 antibody [1229] (AB252224)

4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A-204 (human muscle rhabdomyosarcoma) cells stained for SIX1 (Green) using ab252224 at 10.9μg/ml, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. ab195889 anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used as a counterstain at 1/200 dilution. Confocal image showing nuclear staining in A-204 cell line.
Negative control : Caco-2.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1229] (AB252224)

Formalin-fixed paraffin-embedded human lung tissue stained for SIX1 using ab252224 at 5.45 μg/ml followed by a ready to use secondary antibody.

Nuclear staining on human lung tissue.

The section was incubated with ab252224 for 30 mins at room temperature.

The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1229] (AB252224)

Formalin-fixed paraffin-embedded human skeletal muscle tissue stained for SIX1 using ab252224 at 5.45μg/ml followed by a ready to use secondary antibody.

Nuclear staining on human skeletal muscle tissue.

The section was incubated with ab252224 for 30 mins at room temperature.

The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1229] (AB252224)

Formalin-fixed paraffin-embedded human prostatic hyperplasia tissue stained for SIX1 using ab252224 at 5.45μg/ml followed by a ready to use secondary antibody.

Nuclear staining on human prostatic hyperplasia.

The section was incubated with ab252224 for 30 mins at room temperature.

The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• WB

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Western blot - Anti-SIX1 antibody [1229] (AB252224)

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SIX1 antibody [1229] (ab252224) at 0.545 µg/mL

Lane 1:

A-204 (human muscle rhabdomyosarcoma) whole cell lysate at 20 µg

Lane 2:

Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 32 kDa

true

Exposure time: 3min