Anti-SIX1 antibody [1229] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SIX1 antibody [1229] - BSA and Azide free (AB255759)

4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A-204 (human muscle rhabdomyosarcoma) cells stained for SIX1 (Green) using ab252224 at 10.9μg/ml, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. ab195889 anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used as a counterstain at 1/200 dilution. Confocal image showing nuclear staining in A-204 cell line.
Negative control : Caco-2.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252224).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1229] - BSA and Azide free (AB255759)

Formalin-fixed paraffin-embedded human lung tissue stained for SIX1 using ab252224 at 5.45μg/ml followed by a ready to use secondary antibody.

Nuclear staining on human lung tissue.

The section was incubated with ab252224 for 30 mins at room temperature.

The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252224).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1229] - BSA and Azide free (AB255759)

Formalin-fixed paraffin-embedded human skeletal muscle tissue stained for SIX1 using ab252224 at 5.45μg/ml followed by a ready to use secondary antibody.

Nuclear staining on human skeletal muscle tissue.

The section was incubated with ab252224 for 30 mins at room temperature.

The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252224).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1229] - BSA and Azide free (AB255759)

Formalin-fixed paraffin-embedded human prostatic hyperplasia tissue stained for SIX1 using ab252224 at 5.45μg/ml followed by a ready to use secondary antibody.

Nuclear staining on human prostatic hyperplasia.

The section was incubated with ab252224 for 30 mins at room temperature.

The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252224).

• WB

Supplier Data

Western blot - Anti-SIX1 antibody [1229] - BSA and Azide free (AB255759)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252224).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SIX1 antibody [1229] (<a href='/en-us/products/primary-antibodies/six1-antibody-1229-ab252224'>ab252224</a>) at 0.545 µg/mL

Lane 1:

A-204 (human muscle rhabdomyosarcoma) whole cell lysate at 20 µg

Lane 2:

Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 32 kDa

true

Exposure time: 3min