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Mouse Recombinant Monoclonal SIX1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (AB243247), expandable thumbnail
  • Western blot - Anti-SIX1 antibody [1593] (AB243247), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (AB243247), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (AB243247), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Not recommended
Tested
Mouse
Expected
Not recommended
Tested
Rat
Expected
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
0.459 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
2.295 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
2.295 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
2.295 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development (By similarity). Plays an important role in the development of several organs, including kidney, muscle and inner ear (By similarity). Depending on context, functions as a transcriptional repressor or activator (By similarity). Lacks an activation domain, and requires interaction with EYA family members for transcription activation (PubMed:15141091). Mediates nuclear translocation of EYA1 and EYA2 (PubMed:19497856). Binds the 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the MYOG promoter and CIDEA enhancer (PubMed:15141091, PubMed:19497856, PubMed:23435380, PubMed:27923061). Regulates the expression of numerous genes, including MYC, CCND1 and EZR (By similarity). Acts as an activator of the IGFBP5 promoter, probably coactivated by EYA2 (By similarity). Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex (By similarity). During myogenesis, seems to act together with EYA2 and DACH2 (By similarity). Regulates the expression of CCNA1 (PubMed:15123840). Promotes brown adipocyte differentiation (By similarity).

Alternative names

Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX1

Recommended products

Mouse Recombinant Monoclonal SIX1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
1593
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SIX1 also known as Sine oculis homeobox homolog 1 is a transcription factor with a molecular mass of about 32 kDa. SIX1 plays an important role in regulating gene expression by binding to DNA sequences. This transcription factor shows expression in various tissues including skeletal muscle kidney and neural tissues. In developing tissues the expression is particularly significant indicating its role during embryonic development and differentiation.

Biological function summary

SIX1 influences cell proliferation survival and differentiation. As part of a transcriptional complex it interacts frequently with cofactors like EYA1 to execute its roles in developmental processes. These interactions allow SIX1 to impact gene targets that are important for tissue morphogenesis. The malfunctioning of these processes can lead to developmental disorders highlighting its role in the regulatory networks that govern organogenesis.

Pathways

Several essential biological processes involve SIX1 including the Wnt/β-catenin and TGF-β signaling pathways. In these pathways SIX1 sometimes regulates or interacts with proteins such as β-catenin enhancing transcriptional activation essential for cell fate determination. In the TGF-β pathway SIX1 influences epithelial-mesenchymal transition (EMT) an important event in embryogenesis and metastasis. These pathway involvements indicate its importance in maintaining cellular homeostasis and signaling balance.

Associated diseases and disorders

SIX1 has a significant link to cancer and congenital anomalies. Overexpression or mutations of SIX1 are often implicated in different cancers such as breast cancer due to its role in facilitating EMT and increasing invasiveness. Moreover SIX1 mutations associate with Branchio-Oto-Renal (BOR) syndrome a developmental disorder that affects the kidneys ears and branchial arches. In these contexts SIX1 connects with other proteins like β-catenin in cancer pathways and EYA1 in BOR syndrome further illustrating its broad impact on health and disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (ab243247), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (ab243247)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling SIX1 with ab243247 at 2.295μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Nuclear staining on human skeletal muscle tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-SIX1 antibody [1593] (ab243247), expandable thumbnail

    Western blot - Anti-SIX1 antibody [1593] (ab243247)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SIX1 antibody [1593] (ab243247) at 0.459 µg/mL

    Lane 1: A-204 (human muscle rhabdomyosarcoma), whole cell lysate at 20 µg

    Lane 2: Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 32 kDa

    Observed band size: 32 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (ab243247), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (ab243247)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling SIX1 with ab243247 at 2.295μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Nuclear staining on mouse skeletal muscle tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (ab243247), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (ab243247)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling SIX1 with ab243247 at 2.295μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Nuclear staining on rat skeletal muscle tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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