AB243247

Anti-SIX1 antibody [1593]

Recombinant
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(1 Publication)

Mouse Recombinant Monoclonal SIX1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (AB243247)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (AB243247)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling SIX1 with ab243247 at 2.295μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on human skeletal muscle tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (AB243247)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling SIX1 with ab243247 at 2.295μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on mouse skeletal muscle tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (AB243247)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling SIX1 with ab243247 at 2.295μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat skeletal muscle tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Supplier Data

Western blot - Anti-SIX1 antibody [1593] (AB243247)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SIX1 antibody [1593] (ab243247) at 0.459 µg/mL

Lane 1:

A-204 (human muscle rhabdomyosarcoma), whole cell lysate at 20 µg

Lane 2:

Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 3min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1593

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.459 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.295 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.295 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.295 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIX1 also known as Sine oculis homeobox homolog 1 is a transcription factor with a molecular mass of about 32 kDa. SIX1 plays an important role in regulating gene expression by binding to DNA sequences. This transcription factor shows expression in various tissues including skeletal muscle kidney and neural tissues. In developing tissues the expression is particularly significant indicating its role during embryonic development and differentiation.

Biological function summary

SIX1 influences cell proliferation survival and differentiation. As part of a transcriptional complex it interacts frequently with cofactors like EYA1 to execute its roles in developmental processes. These interactions allow SIX1 to impact gene targets that are important for tissue morphogenesis. The malfunctioning of these processes can lead to developmental disorders highlighting its role in the regulatory networks that govern organogenesis.

Pathways

Several essential biological processes involve SIX1 including the Wnt/β-catenin and TGF-β signaling pathways. In these pathways SIX1 sometimes regulates or interacts with proteins such as β-catenin enhancing transcriptional activation essential for cell fate determination. In the TGF-β pathway SIX1 influences epithelial-mesenchymal transition (EMT) an important event in embryogenesis and metastasis. These pathway involvements indicate its importance in maintaining cellular homeostasis and signaling balance.

SIX1 has a significant link to cancer and congenital anomalies. Overexpression or mutations of SIX1 are often implicated in different cancers such as breast cancer due to its role in facilitating EMT and increasing invasiveness. Moreover SIX1 mutations associate with Branchio-Oto-Renal (BOR) syndrome a developmental disorder that affects the kidneys ears and branchial arches. In these contexts SIX1 connects with other proteins like β-catenin in cancer pathways and EYA1 in BOR syndrome further illustrating its broad impact on health and disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development (By similarity). Plays an important role in the development of several organs, including kidney, muscle and inner ear (By similarity). Depending on context, functions as a transcriptional repressor or activator (By similarity). Lacks an activation domain, and requires interaction with EYA family members for transcription activation (PubMed : 15141091). Mediates nuclear translocation of EYA1 and EYA2 (PubMed : 19497856). Binds the 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the MYOG promoter and CIDEA enhancer (PubMed : 15141091, PubMed : 19497856, PubMed : 23435380, PubMed : 27923061). Regulates the expression of numerous genes, including MYC, CCND1 and EZR (By similarity). Acts as an activator of the IGFBP5 promoter, probably coactivated by EYA2 (By similarity). Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex (By similarity). During myogenesis, seems to act together with EYA2 and DACH2 (By similarity). Regulates the expression of CCNA1 (PubMed : 15123840). Promotes brown adipocyte differentiation (By similarity).

See full target information Homeobox protein SIX1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:8806-8822 PubMed35287543

2022

Hsa_circ_0017639 regulates cisplatin resistance and tumor growth via acting as a miR-1296-5p molecular sponge and modulating sine oculis homeobox 1 expression in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feiyun Chang,Jiali Li,Quan Sun,Shuqing Wei,Yongming Song

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SIX1 antibody [1593]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (AB243247)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (AB243247)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] (AB243247)

Western blot - Anti-SIX1 antibody [1593] (AB243247)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise