Mouse Recombinant Monoclonal SIX1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (AB255754), expandable thumbnail](https://content.abcam.com/products/images/six1-antibody-1593-bsa-and-azide-free-ab255754--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117552.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (AB255754)")
![Western blot - Anti-SIX1 antibody [1593] - BSA and Azide free (AB255754), expandable thumbnail](https://content.abcam.com/products/images/six1-antibody-1593-bsa-and-azide-free-ab255754--western-blot-img12163.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SIX1 antibody [1593] - BSA and Azide free (AB255754)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (AB255754), expandable thumbnail](https://content.abcam.com/products/images/six1-antibody-1593-bsa-and-azide-free-ab255754--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113027.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (AB255754)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (AB255754), expandable thumbnail](https://content.abcam.com/products/images/six1-antibody-1593-bsa-and-azide-free-ab255754--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135543.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (AB255754)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
| Mouse | Expected | Not recommended | Tested |
| Rat | Expected | Not recommended | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.459 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2.295 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 2.295 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 2.295 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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3 products for Alternative Product
Target data
Function
Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development (By similarity). Plays an important role in the development of several organs, including kidney, muscle and inner ear (By similarity). Depending on context, functions as a transcriptional repressor or activator (By similarity). Lacks an activation domain, and requires interaction with EYA family members for transcription activation (PubMed:15141091). Mediates nuclear translocation of EYA1 and EYA2 (PubMed:19497856). Binds the 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the MYOG promoter and CIDEA enhancer (PubMed:15141091, PubMed:19497856, PubMed:23435380, PubMed:27923061). Regulates the expression of numerous genes, including MYC, CCND1 and EZR (By similarity). Acts as an activator of the IGFBP5 promoter, probably coactivated by EYA2 (By similarity). Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex (By similarity). During myogenesis, seems to act together with EYA2 and DACH2 (By similarity). Regulates the expression of CCNA1 (PubMed:15123840). Promotes brown adipocyte differentiation (By similarity).
Alternative names
Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX1
Recommended products
Mouse Recombinant Monoclonal SIX1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 1593
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab255754 is the carrier-free version of Anti-SIX1 antibody [1593] ab243247.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SIX1 also known as Sine oculis homeobox homolog 1 is a transcription factor with a molecular mass of about 32 kDa. SIX1 plays an important role in regulating gene expression by binding to DNA sequences. This transcription factor shows expression in various tissues including skeletal muscle kidney and neural tissues. In developing tissues the expression is particularly significant indicating its role during embryonic development and differentiation.
- Biological function summary
SIX1 influences cell proliferation survival and differentiation. As part of a transcriptional complex it interacts frequently with cofactors like EYA1 to execute its roles in developmental processes. These interactions allow SIX1 to impact gene targets that are important for tissue morphogenesis. The malfunctioning of these processes can lead to developmental disorders highlighting its role in the regulatory networks that govern organogenesis.
- Pathways
Several essential biological processes involve SIX1 including the Wnt/β-catenin and TGF-β signaling pathways. In these pathways SIX1 sometimes regulates or interacts with proteins such as β-catenin enhancing transcriptional activation essential for cell fate determination. In the TGF-β pathway SIX1 influences epithelial-mesenchymal transition (EMT) an important event in embryogenesis and metastasis. These pathway involvements indicate its importance in maintaining cellular homeostasis and signaling balance.
- Associated diseases and disorders
SIX1 has a significant link to cancer and congenital anomalies. Overexpression or mutations of SIX1 are often implicated in different cancers such as breast cancer due to its role in facilitating EMT and increasing invasiveness. Moreover SIX1 mutations associate with Branchio-Oto-Renal (BOR) syndrome a developmental disorder that affects the kidneys ears and branchial arches. In these contexts SIX1 connects with other proteins like β-catenin in cancer pathways and EYA1 in BOR syndrome further illustrating its broad impact on health and disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754), expandable thumbnail](https://content.abcam.com/products/images/six1-antibody-1593-bsa-and-azide-free-ab255754--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117552.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling SIX1 with Anti-SIX1 antibody [1593] ab243247 at 2.295μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Nuclear staining on human skeletal muscle tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (Anti-SIX1 antibody [1593] ab243247).
![Western blot - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754), expandable thumbnail](https://content.abcam.com/products/images/six1-antibody-1593-bsa-and-azide-free-ab255754--western-blot-img12163.jpg "Western blot - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)")
Western blot - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)
Blocking/Dilution buffer: 5% NFDM/TBST.
This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (Anti-SIX1 antibody [1593] ab243247).
All lanes: Western blot - Anti-SIX1 antibody [1593] (Anti-SIX1 antibody [1593] ab243247) at 0.459 µg/mL
Lane 1: A-204 (human muscle rhabdomyosarcoma), whole cell lysate at 20 µg
Lane 2: Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754), expandable thumbnail](https://content.abcam.com/products/images/six1-antibody-1593-bsa-and-azide-free-ab255754--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113027.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling SIX1 with Anti-SIX1 antibody [1593] ab243247 at 2.295μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Nuclear staining on mouse skeletal muscle tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This image was produced using the same antibody clone but in a different formulation Anti-SIX1 antibody [1593] ab243247, PBS, sodium azide, glycerol and BSA.
This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (Anti-SIX1 antibody [1593] ab243247).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754), expandable thumbnail](https://content.abcam.com/products/images/six1-antibody-1593-bsa-and-azide-free-ab255754--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135543.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling SIX1 with Anti-SIX1 antibody [1593] ab243247 at 2.295μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Nuclear staining on rat skeletal muscle tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This image was produced using the same antibody clone but in a different formulation Anti-SIX1 antibody [1593] ab243247, PBS, sodium azide, glycerol and BSA.
This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (Anti-SIX1 antibody [1593] ab243247).
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