Rabbit Polyclonal SIX2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SIX2 aa 250 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SIX2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NPC8
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100 |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcription factor that plays an important role in the development of several organs, including kidney, skull and stomach. During kidney development, maintains cap mesenchyme multipotent nephron progenitor cells in an undifferentiated state by opposing the inductive signals emanating from the ureteric bud and cooperates with WNT9B to promote renewing progenitor cells proliferation. Acts through its interaction with TCF7L2 and OSR1 in a canonical Wnt signaling independent manner preventing transcription of differentiation genes in cap mesenchyme such as WNT4. Also acts independently of OSR1 to activate expression of many cap mesenchyme genes, including itself, GDNF and OSR1. During craniofacial development plays a role in growth and elongation of the cranial base through regulation of chondrocyte differentiation. During stomach organogenesis, controls pyloric sphincter formation and mucosal growth through regulation of a gene network including NKX2-5, BMPR1B, BMP4, SOX9 and GREM1. During branchial arch development, acts to mediate HOXA2 control over the insulin-like growth factor pathway. May also be involved in limb tendon and ligament development (By similarity). Plays a role in cell proliferation and migration.
Alternative names
Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX2
Recommended products
Rabbit Polyclonal SIX2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SIX2 aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SIX2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NPC8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SIX2 also known as Sine Oculis Homeobox Homolog 2 is a transcription factor with a molecular weight of approximately 33 kDa. It contains a homeobox domain that allows binding to specific DNA sequences to regulate gene expression. SIX2 is mainly expressed in the developing kidney and plays an essential role during organogenesis. It is also found in some adult tissues but at lower levels compared to development stages.
- Biological function summary
SIX2 influences cell proliferation and differentiation mostly during early kidney development. It partners with co-activators and is an integral component of developmental signaling complexes. In this context SIX2 helps maintain progenitor cell populations in an undifferentiated state which is vital for proper organ formation and function.
- Pathways
SIX2 plays an essential role in pathways like Wnt and Pax signaling. These pathways regulate important developmental processes by interacting with proteins such as TCF/LEF and PAX2. Through these interactions SIX2 contributes to controlling cell fate decisions and organ patterning during embryogenesis ensuring proper organ development.
- Associated diseases and disorders
SIX2 is associated with renal agenesis and certain types of congenital abnormalities affecting the kidney and urinary tract. Disruptions in the SIX2 expression or function might lead to the absence of kidney formation. It is also linked to pathological conditions interacting with proteins such as GDNF and RET which can contribute to irregular kidney development or diseases involving kidney hyperplasia.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SIX2 antibody (ab220826)
All lanes: Western blot - Anti-SIX2 antibody (ab220826) at 0.4 µg/mL
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2: A431 (Human epidermoid carcinoma cell line) whole cell lysate
Predicted band size: 32 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SIX2 antibody (ab220826)
Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling SIX2 (green) in the nuclear membrane with ab220826 at 2 μg/ml.
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