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AB220826

Anti-SIX2 antibody

4

(1 Review)

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(1 Publication)

Rabbit Polyclonal SIX2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SIX2 aa 250 to C-terminus.

View Alternative Names

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SIX2 antibody (AB220826)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SIX2 antibody (AB220826)

Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling SIX2 (green) in the nuclear membrane with ab220826 at 2 μg/ml.

Western blot - Anti-SIX2 antibody (AB220826)
  • WB

Supplier Data

Western blot - Anti-SIX2 antibody (AB220826)

All lanes:

Western blot - Anti-SIX2 antibody (ab220826) at 0.4 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

A431 (Human epidermoid carcinoma cell line) whole cell lysate

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SIX2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NPC8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIX2 also known as Sine Oculis Homeobox Homolog 2 is a transcription factor with a molecular weight of approximately 33 kDa. It contains a homeobox domain that allows binding to specific DNA sequences to regulate gene expression. SIX2 is mainly expressed in the developing kidney and plays an essential role during organogenesis. It is also found in some adult tissues but at lower levels compared to development stages.
Biological function summary

SIX2 influences cell proliferation and differentiation mostly during early kidney development. It partners with co-activators and is an integral component of developmental signaling complexes. In this context SIX2 helps maintain progenitor cell populations in an undifferentiated state which is vital for proper organ formation and function.

Pathways

SIX2 plays an essential role in pathways like Wnt and Pax signaling. These pathways regulate important developmental processes by interacting with proteins such as TCF/LEF and PAX2. Through these interactions SIX2 contributes to controlling cell fate decisions and organ patterning during embryogenesis ensuring proper organ development.

SIX2 is associated with renal agenesis and certain types of congenital abnormalities affecting the kidney and urinary tract. Disruptions in the SIX2 expression or function might lead to the absence of kidney formation. It is also linked to pathological conditions interacting with proteins such as GDNF and RET which can contribute to irregular kidney development or diseases involving kidney hyperplasia.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that plays an important role in the development of several organs, including kidney, skull and stomach. During kidney development, maintains cap mesenchyme multipotent nephron progenitor cells in an undifferentiated state by opposing the inductive signals emanating from the ureteric bud and cooperates with WNT9B to promote renewing progenitor cells proliferation. Acts through its interaction with TCF7L2 and OSR1 in a canonical Wnt signaling independent manner preventing transcription of differentiation genes in cap mesenchyme such as WNT4. Also acts independently of OSR1 to activate expression of many cap mesenchyme genes, including itself, GDNF and OSR1. During craniofacial development plays a role in growth and elongation of the cranial base through regulation of chondrocyte differentiation. During stomach organogenesis, controls pyloric sphincter formation and mucosal growth through regulation of a gene network including NKX2-5, BMPR1B, BMP4, SOX9 and GREM1. During branchial arch development, acts to mediate HOXA2 control over the insulin-like growth factor pathway. May also be involved in limb tendon and ligament development (By similarity). Plays a role in cell proliferation and migration.
See full target information SIX2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 3:e158 PubMed24714423

2014

Small interfering RNA targeting nerve growth factor alleviates allergic airway hyperresponsiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Lien Chen,Hsin-Ying Huang,Chen-Chen Lee,Bor-Luen Chiang
View all publications
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Product promise

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