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AB75345

Anti-SKA2 antibody

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(4 Publications)

Rabbit Polyclonal SKA2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SKA2.

View Alternative Names

FAM33A, SKA2, Spindle and kinetochore-associated protein 2, Protein FAM33A

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SKA2. The exact immunogen used to generate this antibody is proprietary information.

Q8WVK7

Specificity

ab75345 reacts specifically with SKA2 protein

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Primary Antibodies

AB91550

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Primary Antibodies

AB186003

Anti-SKA3 antibody - C-terminal

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Primary Antibodies

AB70802

Anti-ZC3H13 antibody

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SKA2 also known as spindle and kinetochore-associated protein 2 is an important component in the regulation of cell division. It has a mass of approximately 18 kDa and is expressed in tissues with high proliferative capacity such as the brain and liver. Mechanically SKA2 interacts with microtubules at the mitotic spindle and aids in the stabilization of kinetochore-microtubule attachments during mitosis. This interaction ensures proper chromosome segregation which is essential for successful cell division.
Biological function summary

This protein is part of the SKA complex which also includes SKA1 and SKA3. This complex stabilizes spindle fibers to enhance the attachment of chromosomes during mitosis. The SKA complex functions to maintain chromosomal alignment and ensure that sister chromatids separate correctly. Disruption of SKA2's role in this process can lead to chromosomal instability a factor often associated with various pathological conditions.

Pathways

SKA2 plays a significant role in the cell cycle and spindle assembly checkpoint pathways. The spindle assembly checkpoint ensures that cells do not proceed to anaphase until all chromosomes are properly aligned. SKA2 is closely related to the Ndc80 complex in these pathways. Proper function of these pathways is critical for maintaining genomic integrity and preventing aneuploidy a condition where cells have an abnormal number of chromosomes.

SKA2 has gathered attention due to its association with cancer and psychiatric disorders. Dysregulation of SKA2 contributes to tumorigenesis as it can lead to uncontrolled cell proliferation and chromosomal missegregation. In psychiatric contexts altered SKA2 expression has been linked to stress-related disorders including depression. SKA2 interacts with proteins such as FKBP5 in these disorders influencing glucocorticoid receptor sensitivity and stress response.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed : 17093495, PubMed : 19289083, PubMed : 23085020). Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (PubMed : 17093495). The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (PubMed : 19289083). The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (PubMed : 17093495, PubMed : 19289083). In the complex, it is required for SKA1 localization (PubMed : 19289083). Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed : 23085020).
See full target information SKA2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Thoracic cancer 14:1707-1718 PubMed37132052

2023

Circ_0001387 regulates SKA2 to accelerate breast cancer progression through miR-136-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Youyi Xiong,Lin Li,Nan Wang,Fang Wang,Zhuo Chen,Luhong Han,Shan Jiang,Yuanting Gu

Molecular oncology 15:697-709 PubMed33244865

2020

Circ_0008039 supports breast cancer cell proliferation, migration, invasion, and glycolysis by regulating the miR-140-3p/SKA2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dongwei Dou,Xiaoyang Ren,Mingli Han,Xiaodong Xu,Xin Ge,Yuanting Gu,Xinxing Wang,Song Zhao

Neoplasma 67:743-750 PubMed32241158

2020

SKA2 promotes proliferation and invasion of hepatocellular carcinoma cells via activating the β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

D Wang,Y J Suo,L Gong,S Q Lv

Nature 534:335-40 PubMed27306184

2016

Stem cell function and stress response are controlled by protein synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Blanco,Roberto Bandiera,Martyna Popis,Shobbir Hussain,Patrick Lombard,Jelena Aleksic,Abdulrahim Sajini,Hinal Tanna,Rosana Cortés-Garrido,Nikoletta Gkatza,Sabine Dietmann,Michaela Frye
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Product promise

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