Anti-SKA3 antibody - C-terminal

• IP

Supplier Data

Immunoprecipitation - Anti-SKA3 antibody - C-terminal (AB186003)

Detection of Human SKA3 by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP Jurkat whole cell lysate loaded. ab186003 used for IP at 6μg/ml. For blotting immunoprecipitated SKA3, ab186003 was used at 1 μg/ml.

All lanes:

Immunoprecipitation - Anti-SKA3 antibody - C-terminal (ab186003)

Predicted band size: 46 kDa

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Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-SKA3 antibody - C-terminal (AB186003)

All lanes:

Western blot - Anti-SKA3 antibody - C-terminal (ab186003) at 0.1 µg/mL

Lane 1:

HeLa lysate at 50 µg

Lane 2:

293T lysate at 50 µg

Lane 3:

Jurkat lysate at 50 µg

Predicted band size: 46 kDa

true

Exposure time: 30s

• WB

CiteAb

Western blot - Anti-SKA3 antibody - C-terminal (AB186003)

SKA3 western blot using anti-SKA3 antibody - C-terminal ab186003. Publication image and figure legend from Hu, D. D., Chen, H. L., et al., 2020, Biosci Rep, PubMed 32068236.

ab186003 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab186003 please see the product overview.

SKA3 is up-regulated in LUAD(A) Gene Expression Profiling Interactive Analysis (GEPIA) indicated that the SKA3 expression is enhanced in LUAD tissues compared with normal tissue (fold-change > 2, P-value < 0.05). (B) SKA3 mRNA in 26 paired LUADs and ANTs examined by qRT-PCR. Data were compared via a Student's t test. (C) SKA3 mRNA in 19 LUADs lacking lymph node metastasis and 7 with lymph node metastasis. (D) SKA3 expression in MRC-5, H226 and SK-MES-1 cells. (E) GEPIA analysis revealing the association of high SKA3 expression with a poor OS. Data were compared via two-sided log-rank tests. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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