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AB122769

Anti-SKAP antibody

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(4 Publications)

Rabbit Polyclonal SKAP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KNSTRN aa 50-200.

View Alternative Names

C15orf23, SKAP, TRAF4AF1, HSD11, KNSTRN, Small kinetochore-associated protein, Kinetochore-localized astrin-binding protein, Kinetochore-localized astrin/SPAG5-binding protein, TRAF4-associated factor 1, Kinastrin

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SKAP antibody (AB122769)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SKAP antibody (AB122769)

Immunofluorescent staining of Human cell line A-431 shows positivity in cytosol and microtubules. Recommended concentration of ab122769 1-4 μg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKAP antibody (AB122769)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKAP antibody (AB122769)

ab122769, at 1/100 dilution, staining SKAP in paraffin-embedded Human testis tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KNSTRN aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q9Y448

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SKAP also known as Src Kinase-Associated Phosphoprotein of 55 kDa is an adaptor protein that plays an important role in T cell activation. It weighs approximately 55 kDa and is prominently expressed in lymphoid tissues where it supports the immune response. Functionally SKAP forms part of signaling cascades that transmit extracellular signals into the cell interior modulating cellular activities.
Biological function summary

SKAP regulates immune cell function by influencing adhesion and migration processes. It often forms part of larger complexes within the immune synapse facilitating interactions between proteins necessary for efficient signaling. SKAP's role in T cells involves partnering with other signaling molecules to strengthen immune responses against infections by promoting cell adhesion.

Pathways

SKAP participates actively in the T-cell receptor (TCR) signaling and integrin-mediated pathways. It associates with proteins such as LFA-1 an important integrin that mediates cell adhesion. SKAP affects the downstream signaling events by modulating key interactions and conformations which are important for effective immune response initiation and regulation.

SKAP has notable connections with autoimmune diseases like rheumatoid arthritis and multiple sclerosis. Its deregulation affects the immune system's balance leading to exacerbated inflammatory responses. Connections to proteins like RapL which interacts with integrins to regulate cell adhesion and migration imply its involvements in pathological conditions involving the immune system's improper regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential component of the mitotic spindle required for faithful chromosome segregation and progression into anaphase (PubMed : 19667759). Promotes the metaphase-to-anaphase transition and is required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed : 19667759, PubMed : 22110139). The astrin (SPAG5)-kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments (PubMed : 21402792). Required for kinetochore oscillations and dynamics of microtubule plus-ends during live cell mitosis, possibly by forming a link between spindle microtubule plus-ends and mitotic chromosomes to achieve faithful cell division (PubMed : 23035123). May be involved in UV-induced apoptosis via its interaction with PRPF19; however, these results need additional evidences (PubMed : 24718257).
See full target information KNSTRN
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:724860 PubMed34568334

2021

Integrative Analysis of Biomarkers Through Machine Learning Identifies Stemness Features in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Wei,Jichuan Quan,Shuofeng Li,Hengchang Liu,Xu Guan,Zheng Jiang,Xishan Wang

Frontiers in oncology 9:1147 PubMed31781484

2019

Clinical and Expression Significance of AKT1 by Co-expression Network Analysis in Endometrial Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Huo,Hengzi Sun,Qian Liu,Xiangwen Ma,Peng Peng,Mei Yu,Ying Zhang,Dongyan Cao,Keng Shen

Nature genetics 46:1060-2 PubMed25194279

2014

Recurrent point mutations in the kinetochore gene KNSTRN in cutaneous squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Carolyn S Lee,Aparna Bhaduri,Angela Mah,Whitney L Johnson,Alexander Ungewickell,Cody J Aros,Christie B Nguyen,Eon J Rios,Zurab Siprashvili,Aaron Straight,Jinah Kim,Sumaira Z Aasi,Paul A Khavari

PloS one 9:e92712 PubMed24718257

2014

Small kinetochore associated protein (SKAP) promotes UV-induced cell apoptosis through negatively regulating pre-mRNA processing factor 19 (Prp19).

Applications

WB

Species

Human

Shan Lu,Renxian Wang,Congli Cai,Junbo Liang,Longchang Xu,Shiying Miao,Linfang Wang,Wei Song
View all publications
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Product promise

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