AB170882

Anti-SKAP55 antibody [EPR11360]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal SKAP55 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

SCAP1, SKAP55, SKAP1, Src kinase-associated phosphoprotein 1, Src family-associated phosphoprotein 1, Src kinase-associated phosphoprotein of 55 kDa, SKAP-55, pp55

3 Images

Flow Cytometry (Intracellular) - Anti-SKAP55 antibody [EPR11360] (AB170882)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SKAP55 antibody [EPR11360] (AB170882)

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling SKAP55using ab170882 at a 1/10 dilution (red) or a rabbit IgG (green).

Immunocytochemistry/ Immunofluorescence - Anti-SKAP55 antibody [EPR11360] (AB170882)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SKAP55 antibody [EPR11360] (AB170882)

Immunofluorescent staining of Jurkat cells labeling SKAP55 using ab170882 at a 1/100 dilution (green) and DAPI nuclear staining (blue).

Supplier Data

Western blot - Anti-SKAP55 antibody [EPR11360] (AB170882)

All lanes:

Western blot - Anti-SKAP55 antibody [EPR11360] (ab170882) at 1/1000 dilution

Lane 1:

Human thymus lysate at 10 µg

Lane 2:

Jurkat lysate at 10 µg

Lane 3:

HuT-78 lysate at 10 µg

Lane 4:

MOLT4 lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 41 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11360

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SKAP55 also known as Src kinase-associated phosphoprotein 55 is a protein with a mass of approximately 55 kDa. It plays an important role in T-cell signaling. This protein mainly expresses in lymphoid tissues including the thymus spleen and peripheral blood lymphocytes. SKAP55 interacts with signaling molecules via its SH3 domain facilitating cellular communication and activation.

Biological function summary

The protein functions as an adapter molecule in T-cell receptor (TCR) signaling pathways. SKAP55 associates with the LAT-Gads complex contributing to intracellular signal transduction. It helps mediate actin cytoskeletal rearrangements necessary for immune synapse formation. These rearrangements are critical for effective immune response meaning SKAP55 is integral to T-cell activation and function.

Pathways

SKAP55 participates in the signaling events downstream of the TCR particularly in the PI3K and Ras-MEK-ERK pathways. It works closely with proteins like LCK and ZAP70 which are essential for the phosphorylation processes involved in T-cell activation. Through these interactions SKAP55 influences the organization of signaling assemblies promoting effective cellular responses.

SKAP55 relates to immune system-related conditions like autoimmune diseases and immunodeficiencies. Alterations in SKAP55 expression or function can affect T-cell signaling potentially leading to improper immune responses. SKAP55 interactions with LCK can influence diseases such as systemic lupus erythematosus where immune regulation is disrupted highlighting its importance in maintaining immune system balance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.

See full target information SKAP1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 39:680-694 PubMed37647346

2023

Generalized machine learning based on multi-omics data to profile the effect of ferroptosis pathway on prognosis and immunotherapy response in patients with bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Liu,Ziran Qiu,Xiongfeng Zhang,Zhouhua Su,Renzheng Yi,Debo Zou,Chaoqun Xie,Na Jin,Weibing Long,Xiaobing Liu

The Journal of biological chemistry 294:4815-4827 PubMed30659097

2019

Hepatitis B virus X protein-induced SH2 domain-containing 5 (SH2D5) expression promotes hepatoma cell growth via an SH2D5-transketolase interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Yongfa Zheng,Pingpo Ming,Chengliang Zhu,Yu Si,Shilei Xu,Aidong Chen,Jun Wang,Binghong Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SKAP55 antibody [EPR11360]

Flow Cytometry (Intracellular) - Anti-SKAP55 antibody [EPR11360] (AB170882)

Immunocytochemistry/ Immunofluorescence - Anti-SKAP55 antibody [EPR11360] (AB170882)

Western blot - Anti-SKAP55 antibody [EPR11360] (AB170882)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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