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AB249562

Anti-SKAP55 antibody [EPR11360] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SKAP55 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SCAP1, SKAP55, SKAP1, Src kinase-associated phosphoprotein 1, Src family-associated phosphoprotein 1, Src kinase-associated phosphoprotein of 55 kDa, SKAP-55, pp55

3 Images
Flow Cytometry (Intracellular) - Anti-SKAP55 antibody [EPR11360] - BSA and Azide free (AB249562)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SKAP55 antibody [EPR11360] - BSA and Azide free (AB249562)

This data was developed using ab170882, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling SKAP55 using ab170882 at a 1/10 dilution (red) or a rabbit IgG (green).

Immunocytochemistry/ Immunofluorescence - Anti-SKAP55 antibody [EPR11360] - BSA and Azide free (AB249562)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SKAP55 antibody [EPR11360] - BSA and Azide free (AB249562)

This data was developed using ab170882, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of Jurkat cells labeling SKAP55 using ab170882 at a 1/100 dilution (green) and DAPI nuclear staining (blue).

Western blot - Anti-SKAP55 antibody [EPR11360] - BSA and Azide free (AB249562)
  • WB

Supplier Data

Western blot - Anti-SKAP55 antibody [EPR11360] - BSA and Azide free (AB249562)

This data was developed using ab170882, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SKAP55 antibody [EPR11360] (<a href='/en-us/products/primary-antibodies/skap55-antibody-epr11360-ab170882'>ab170882</a>) at 1/1000 dilution

Lane 1:

Human thymus lysate at 10 µg

Lane 2:

Jurkat lysate at 10 µg

Lane 3:

HuT-78 lysate at 10 µg

Lane 4:

MOLT4 lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11360

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249562 is the carrier-free version of ab170882.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SKAP55 also known as Src kinase-associated phosphoprotein 55 is a protein with a mass of approximately 55 kDa. It plays an important role in T-cell signaling. This protein mainly expresses in lymphoid tissues including the thymus spleen and peripheral blood lymphocytes. SKAP55 interacts with signaling molecules via its SH3 domain facilitating cellular communication and activation.
Biological function summary

The protein functions as an adapter molecule in T-cell receptor (TCR) signaling pathways. SKAP55 associates with the LAT-Gads complex contributing to intracellular signal transduction. It helps mediate actin cytoskeletal rearrangements necessary for immune synapse formation. These rearrangements are critical for effective immune response meaning SKAP55 is integral to T-cell activation and function.

Pathways

SKAP55 participates in the signaling events downstream of the TCR particularly in the PI3K and Ras-MEK-ERK pathways. It works closely with proteins like LCK and ZAP70 which are essential for the phosphorylation processes involved in T-cell activation. Through these interactions SKAP55 influences the organization of signaling assemblies promoting effective cellular responses.

SKAP55 relates to immune system-related conditions like autoimmune diseases and immunodeficiencies. Alterations in SKAP55 expression or function can affect T-cell signaling potentially leading to improper immune responses. SKAP55 interactions with LCK can influence diseases such as systemic lupus erythematosus where immune regulation is disrupted highlighting its importance in maintaining immune system balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.
See full target information SKAP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1162669 PubMed37207208

2023

Naproxen chemoprevention induces proliferation of cytotoxic lymphocytes in Lynch Syndrome colorectal mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Charles M Bowen,Nan Deng,Laura Reyes-Uribe,Edwin Roger Parra,Pedro Rocha,Luisa M Solis,Ignacio I Wistuba,Valerie O Sepeda,Lana Vornik,Marjorie Perloff,Eva Szabo,Asad Umar,Krishna M Sinha,Powel H Brown,Eduardo Vilar
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com