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AB115553

Anti-SKI antibody

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(1 Publication)

Rabbit Polyclonal SKI antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Ski oncogene conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Ski oncogene, Proto-oncogene c-Ski, SKI

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKI antibody (AB115553)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKI antibody (AB115553)

ab115553, at 2.5µg/ml, staining SKI in Formalin-fixed, Paraffin-embedded Human Kidney tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKI antibody (AB115553)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKI antibody (AB115553)

ab115553, at 2.5µg/ml, staining SKI in Formalin-fixed, Paraffin-embedded Human Pancreas tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human Ski oncogene conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P12755

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SKI also known by names such as SLOAN-KETTERING INDERTERMINATE PROTEIN plays a role as a transcriptional co-repressor that affects gene expression. It has a molecular mass of approximately 84 kDa. SKI primarily exhibits expression in tissues like the colon brain and skeletal muscle. This expression pattern suggests its involvement in regulating diverse biological processes in these tissues.
Biological function summary

SKI functions as a negative regulator of the TGF-beta/Smad signaling pathway. It interacts within transcriptional complexes modulating gene transcription through direct binding to SMAD proteins which are intracellular mediators of TGF-beta signals. This binding capability enables SKI to influence the transcription of TGF-beta-inducible genes impacting cell growth and differentiation processes.

Pathways

The involvement of SKI in the TGF-beta signaling cascade is significant. This pathway has important roles in cellular proliferation differentiation and apoptosis. SKI counters the activity of other proteins within this pathway such as SMAD2 and SMAD3 by altering their ability to regulate gene expression. This interaction highlights SKI's part in controlling cellular responses initiated by TGF-beta signals.

SKI's connection to cancer and its progression is noteworthy especially in conditions like leukemia and colorectal cancer. Aberrant SKI expression links to the disruption of normal TGF-beta signaling a condition associated with unchecked cellular proliferation. In leukemia for instance altered SKI activity can interact with the MYC oncogene further influencing cell cycle regulation and promoting malignant transformation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.
See full target information Ski oncogene
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 120:e2213075120 PubMed36791098

2023

The HP1α protein is mandatory to repress the circadian clock and its output genes during the 12 h period of transcriptional repression.

Applications

Unspecified application

Species

Unspecified reactive species

Nisha Misra,Manohar Damara,Pierre Chambon
View all publications
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Product promise

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