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Rabbit Polyclonal SKI antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SKI conjugated to Keyhole Limpet Haemocyanin.

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Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human SKI conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P12755
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species

Human

Dilution info

2.5 µg/mL

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target data

Function

May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.

Alternative names

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Rabbit Polyclonal SKI antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SKI conjugated to Keyhole Limpet Haemocyanin.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human SKI conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P12755
Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

SKI functions as a negative regulator of the TGF-beta/Smad signaling pathway. It interacts within transcriptional complexes modulating gene transcription through direct binding to SMAD proteins which are intracellular mediators of TGF-beta signals. This binding capability enables SKI to influence the transcription of TGF-beta-inducible genes impacting cell growth and differentiation processes.

Activity summary

SKI also known by names such as SLOAN-KETTERING INDERTERMINATE PROTEIN plays a role as a transcriptional co-repressor that affects gene expression. It has a molecular mass of approximately 84 kDa. SKI primarily exhibits expression in tissues like the colon brain and skeletal muscle. This expression pattern suggests its involvement in regulating diverse biological processes in these tissues.

Pathways

The involvement of SKI in the TGF-beta signaling cascade is significant. This pathway has important roles in cellular proliferation differentiation and apoptosis. SKI counters the activity of other proteins within this pathway such as SMAD2 and SMAD3 by altering their ability to regulate gene expression. This interaction highlights SKI's part in controlling cellular responses initiated by TGF-beta signals.

Associated diseases and disorders

SKI's connection to cancer and its progression is noteworthy especially in conditions like leukemia and colorectal cancer. Aberrant SKI expression links to the disruption of normal TGF-beta signaling a condition associated with unchecked cellular proliferation. In leukemia for instance altered SKI activity can interact with the MYC oncogene further influencing cell cycle regulation and promoting malignant transformation.

Product promise

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2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKI antibody (ab115553), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKI antibody (ab115553)

    ab115553, at 2.5µg/ml, staining SKI in Formalin-fixed, Paraffin-embedded Human Kidney tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKI antibody (ab115553), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKI antibody (ab115553)

    ab115553, at 2.5µg/ml, staining SKI in Formalin-fixed, Paraffin-embedded Human Pancreas tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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