AB192520

Anti-SKI antibody

Name: Anti-SKI antibody (ab192520) | Abcam
Brand: Abcam Limited
SKU: AB192520
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal SKI antibody. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Ski oncogene.

View Alternative Names

Ski oncogene, Proto-oncogene c-Ski, SKI

1 Images

Supplier Data

Western blot - Anti-SKI antibody (AB192520)

All lanes:

Western blot - Anti-SKI antibody (ab192520) at 1/500 dilution

All lanes:

Mouse fetal muscle tissue lysate

Predicted band size: 80 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Ski oncogene.

P12755

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purity is > 95% (by SDS-PAGE).

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SKI also known by names such as SLOAN-KETTERING INDERTERMINATE PROTEIN plays a role as a transcriptional co-repressor that affects gene expression. It has a molecular mass of approximately 84 kDa. SKI primarily exhibits expression in tissues like the colon brain and skeletal muscle. This expression pattern suggests its involvement in regulating diverse biological processes in these tissues.

Biological function summary

SKI functions as a negative regulator of the TGF-beta/Smad signaling pathway. It interacts within transcriptional complexes modulating gene transcription through direct binding to SMAD proteins which are intracellular mediators of TGF-beta signals. This binding capability enables SKI to influence the transcription of TGF-beta-inducible genes impacting cell growth and differentiation processes.

Pathways

The involvement of SKI in the TGF-beta signaling cascade is significant. This pathway has important roles in cellular proliferation differentiation and apoptosis. SKI counters the activity of other proteins within this pathway such as SMAD2 and SMAD3 by altering their ability to regulate gene expression. This interaction highlights SKI's part in controlling cellular responses initiated by TGF-beta signals.

SKI's connection to cancer and its progression is noteworthy especially in conditions like leukemia and colorectal cancer. Aberrant SKI expression links to the disruption of normal TGF-beta signaling a condition associated with unchecked cellular proliferation. In leukemia for instance altered SKI activity can interact with the MYC oncogene further influencing cell cycle regulation and promoting malignant transformation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.

See full target information Ski oncogene

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e25227 PubMed38333873

2024

Regulation of PDGFRα cells and ICC in progesterone-mediated slow colon transit in pregnant mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Lu,Hui Luo,Ye Wang,Shuang Jing,Jun Zhao,Kexin Zou,Fan Wu,Hao Ying

PloS one 17:e0270880 PubMed35857794

2022

ATF4 promotes brain vascular smooth muscle cells proliferation, invasion and migration by targeting miR-552-SKI axis.

Applications

Unspecified application

Species

Unspecified reactive species

Meina Feng,Qin Zhou,Wenxian Tu,Yunfeng Wang,Yuanmin Du,Kang Xu

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SKI antibody

Western blot - Anti-SKI antibody (AB192520)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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