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AB327190

Anti-SKIL Antibody

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Rabbit Polyclonal SnoN/SNO antibody. Suitable for WB, IHC-P, ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SKIL.

View Alternative Names

SNO, SKIL, Ski-like protein, Ski-related oncogene, Ski-related protein

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKIL Antibody (AB327190)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKIL Antibody (AB327190)

Immunohistochemical staining of human colon using ab327190 at 1 : 200–1 : 500 dilution, showing strong cytoplasmic positivity in glandular cells.

Immunocytochemistry/ Immunofluorescence - Anti-SKIL Antibody (AB327190)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SKIL Antibody (AB327190)

Immunofluorescent staining of human cell line U-251 MG using ab327190 at 0.25–2 µg/ml, showing localization to nucleoplasm & cytosol.

ChIP - Anti-SKIL Antibody (AB327190)
  • ChIP

Supplier Data

ChIP - Anti-SKIL Antibody (AB327190)

ChIP-Exo-Seq composite graph for ab327190 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Western blot - Anti-SKIL Antibody (AB327190)
  • WB

Supplier Data

Western blot - Anti-SKIL Antibody (AB327190)

Western blot analysis in human cell line NTERA-2 using ab327190 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-SKIL Antibody (ab327190)

All lanes:

Human cell line NTERA-2

Predicted band size: 76.98 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ChIP, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SKIL.

P12757

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

May have regulatory role in cell division or differentiation in response to extracellular signals.
See full target information SKIL

Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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