Rabbit Recombinant Monoclonal SKP1 antibody. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications.
Images
![Western blot - Anti-Skp1 antibody [EPR3304] (AB76502), expandable thumbnail](https://content.abcam.com/products/images/skp1-antibody-epr3304-ab76502--western-blot-img126295.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Skp1 antibody [EPR3304] (AB76502)")
![Flow Cytometry (Intracellular) - Anti-Skp1 antibody [EPR3304] (AB76502), expandable thumbnail](https://content.abcam.com/products/images/skp1-antibody-epr3304-ab76502--flow-cytometry-intracellular-img38004.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Skp1 antibody [EPR3304] (AB76502)")
Publications
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- Neuro-oncology 24:2107-21202022FRK inhibits glioblastoma progression via phosphorylating YAP and inducing its ubiquitylation and degradation by Siah1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Wang,Kai Wang,Jiale Fu,Yu Zhang,Yufei Mao,Xu Wang,Xiang Wang,Rutong Yu,Xiuping ZhouPubMed 35723276
- Blood cancer discovery 3:116-1352022BCOR and BCORL1 Mutations Drive Epigenetic Reprogramming and Oncogenic Signaling by Unlinking PRC1.1 from Target Genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEva J Schaefer,Helen C Wang,Hannah Q Karp,Clifford A Meyer,Paloma Cejas,Micah D Gearhart,Emmalee R Adelman,Iman Fares,Annie Apffel,Klothilda Lim,Yingtian Xie,Christopher J Gibson,Monica Schenone,H Moses Murdock,Eunice S Wang,Lukasz P Gondek,Martin P Carroll,Rahul S Vedula,Eric S Winer,Jacqueline S Garcia,Richard M Stone,Marlise R Luskin,Steven A Carr,Henry W Long,Vivian J Bardwell,Maria E Figueroa,R Coleman LindsleyPubMed 35015684
- Cancer research and treatment 54:525-5402021A Hypoxia-Induced SCFFBXL1 E3 Ligase Ubiquitinates and Degrades the MEN1 Tumor Suppressor to Promote Colorectal Cancer Tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Zeng,Xiao-Qing Xiao,Zhi-Yong ZhouPubMed 34237211
- Cell death discovery 7:1642021The proteolytic landscape of cells exposed to non-lethal stresses is shaped by executioner caspases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría Del Carmen Conde-Rubio,Roman Mylonas,Christian WidmannPubMed 34226511
- Cellular and molecular gastroenterology and hepatology 12:1121-11432021TAK1 Is a Novel Target in Hepatocellular Carcinoma and Contributes to Sorafenib Resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShunjie Xia,Lin Ji,Liye Tao,Yu Pan,Zhongjie Lin,Zhe Wan,Haoqi Pan,Jie Zhao,Liuxin Cai,Junjie Xu,Xiujun CaiPubMed 33962073
- Molecular cancer research : MCR 17:709-7192019The Tumor Suppressor FBW7 and the Vitamin D Receptor Are Mutual Cofactors in Protein Turnover and Transcriptional Regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesReyhaneh Salehi-Tabar et. al.PubMed 30606768
- Journal of virology 88:3464-732014Virulence factor NSs of rift valley fever virus recruits the F-box protein FBXO3 to degrade subunit p62 of general transcription factor TFIIH.Applications:WBReactive species:Unspecified reactive speciesMarkus Kainulainen et. al.PubMed 24403578
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Expected |
| Mouse | Expected | Not recommended | Tested | Not recommended | Expected |
| Rat | Expected | Not recommended | Tested | Not recommended | Expected |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2 (PubMed:25704143). SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2. Core component of the Cul7-RING(FBXW8) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:35982156).
Alternative names
EMC19, OCP2, SKP1A, TCEB1L, SKP1, S-phase kinase-associated protein 1, Cyclin-A/CDK2-associated protein p19, Organ of Corti protein 2, Organ of Corti protein II, RNA polymerase II elongation factor-like protein, SIII, Transcription elongation factor B polypeptide 1-like, p19skp1, p19A, OCP-2, OCP-II
Recommended products
Rabbit Recombinant Monoclonal SKP1 antibody. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3304
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Skp1 antibody [EPR3304] (ab76502), expandable thumbnail](https://content.abcam.com/products/images/skp1-antibody-epr3304-ab76502--western-blot-img126295.jpg "Western blot - Anti-Skp1 antibody [EPR3304] (ab76502)")
Western blot - Anti-Skp1 antibody [EPR3304] (ab76502)
All lanes: Western blot - Anti-Skp1 antibody [EPR3304] (ab76502) at 1/2000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: PC-12 cell lysate at 10 µg
Lane 4: NIH/3T3 cell lysate at 10 µg
SecondaryAll lanes: goat anti-rabbit HRP labeled IgG at 1/2000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
![Flow Cytometry (Intracellular) - Anti-Skp1 antibody [EPR3304] (ab76502), expandable thumbnail](https://content.abcam.com/products/images/skp1-antibody-epr3304-ab76502--flow-cytometry-intracellular-img38004.jpg "Flow Cytometry (Intracellular) - Anti-Skp1 antibody [EPR3304] (ab76502)")
Flow Cytometry (Intracellular) - Anti-Skp1 antibody [EPR3304] (ab76502)
Overlay histogram showing HeLa cells stained with ab76502 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76502, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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