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AB76502

Anti-Skp1 antibody [EPR3304]

5

(2 Reviews)

|

(9 Publications)

Rabbit Recombinant Monoclonal SKP1 antibody. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 9 publications.

View Alternative Names

EMC19, OCP2, SKP1A, TCEB1L, SKP1, S-phase kinase-associated protein 1, Cyclin-A/CDK2-associated protein p19, Organ of Corti protein 2, Organ of Corti protein II, RNA polymerase II elongation factor-like protein, SIII, Transcription elongation factor B polypeptide 1-like, p19skp1, p19A, OCP-2, OCP-II

2 Images
Flow Cytometry (Intracellular) - Anti-Skp1 antibody [EPR3304] (AB76502)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Skp1 antibody [EPR3304] (AB76502)

Overlay histogram showing HeLa cells stained with ab76502 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76502 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Western blot - Anti-Skp1 antibody [EPR3304] (AB76502)
  • WB

Unknown

Western blot - Anti-Skp1 antibody [EPR3304] (AB76502)

All lanes:

Western blot - Anti-Skp1 antibody [EPR3304] (ab76502) at 1/2000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

PC-12 cell lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP labeled IgG at 1/2000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

  • Carrier free

    Anti-Skp1 antibody [EPR3304] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3304

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/1000", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2 (PubMed : 25704143). SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2. Core component of the Cul7-RING(FBXW8) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 35982156).
See full target information SKP1
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:214 PubMed39934243

2025

Inhibition of SCF/USP22-dependent nuclear β-catenin ubiquitylation mediates cerebral ischemic tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Yunyan Zuo,Jiahui Xue,Haixia Wen,Lixuan Zhan,Meiyan Chen,Weiwen Sun,En Xu

Communications biology 7:1099 PubMed39244596

2024

FBXO47 regulates centromere pairing as key component of centromeric SCF E3 ligase in mouse spermatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ani Ma,Yali Yang,Lianbao Cao,Lijun Chen,Jian V Zhang

Neuro-oncology 24:2107-2120 PubMed35723276

2022

FRK inhibits glioblastoma progression via phosphorylating YAP and inducing its ubiquitylation and degradation by Siah1.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Kai Wang,Jiale Fu,Yu Zhang,Yufei Mao,Xu Wang,Xiang Wang,Rutong Yu,Xiuping Zhou

Blood cancer discovery 3:116-135 PubMed35015684

2022

BCOR and BCORL1 Mutations Drive Epigenetic Reprogramming and Oncogenic Signaling by Unlinking PRC1.1 from Target Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Eva J Schaefer,Helen C Wang,Hannah Q Karp,Clifford A Meyer,Paloma Cejas,Micah D Gearhart,Emmalee R Adelman,Iman Fares,Annie Apffel,Klothilda Lim,Yingtian Xie,Christopher J Gibson,Monica Schenone,H Moses Murdock,Eunice S Wang,Lukasz P Gondek,Martin P Carroll,Rahul S Vedula,Eric S Winer,Jacqueline S Garcia,Richard M Stone,Marlise R Luskin,Steven A Carr,Henry W Long,Vivian J Bardwell,Maria E Figueroa,R Coleman Lindsley

Cancer research and treatment 54:525-540 PubMed34237211

2021

A Hypoxia-Induced SCFFBXL1 E3 Ligase Ubiquitinates and Degrades the MEN1 Tumor Suppressor to Promote Colorectal Cancer Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zeng,Xiao-Qing Xiao,Zhi-Yong Zhou

Cell death discovery 7:164 PubMed34226511

2021

The proteolytic landscape of cells exposed to non-lethal stresses is shaped by executioner caspases.

Applications

Unspecified application

Species

Unspecified reactive species

María Del Carmen Conde-Rubio,Roman Mylonas,Christian Widmann

Cellular and molecular gastroenterology and hepatology 12:1121-1143 PubMed33962073

2021

TAK1 Is a Novel Target in Hepatocellular Carcinoma and Contributes to Sorafenib Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Shunjie Xia,Lin Ji,Liye Tao,Yu Pan,Zhongjie Lin,Zhe Wan,Haoqi Pan,Jie Zhao,Liuxin Cai,Junjie Xu,Xiujun Cai

Molecular cancer research : MCR 17:709-719 PubMed30606768

2019

The Tumor Suppressor FBW7 and the Vitamin D Receptor Are Mutual Cofactors in Protein Turnover and Transcriptional Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Reyhaneh Salehi-Tabar,Babak Memari,Hilary Wong,Vassil Dimitrov,Natacha Rochel,John H White

Journal of virology 88:3464-73 PubMed24403578

2014

Virulence factor NSs of rift valley fever virus recruits the F-box protein FBXO3 to degrade subunit p62 of general transcription factor TFIIH.

Applications

WB

Species

Unspecified reactive species

Markus Kainulainen,Matthias Habjan,Philipp Hubel,Laura Busch,Simone Lau,Jacques Colinge,Giulio Superti-Furga,Andreas Pichlmair,Friedemann Weber
View all publications
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Product promise

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