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Rabbit Recombinant Monoclonal SKP2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 10 publications.


Images

Western blot - Anti-SKP2 antibody [EPR3305(2)] (AB183039), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SKP2 antibody [EPR3305(2)] (AB183039), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SKP2 antibody [EPR3305(2)] (AB183039), expandable thumbnail
  • Western blot - Anti-SKP2 antibody [EPR3305(2)] (AB183039), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKP2 antibody [EPR3305(2)] (AB183039), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Expected
Rat
Tested
Expected
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/200
Notes

-

Species
Human
Dilution info
1/200
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/70
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription (PubMed:11931757, PubMed:12435635, PubMed:12769844, PubMed:12840033, PubMed:15342634, PubMed:15668399, PubMed:15949444, PubMed:16103164, PubMed:16262255, PubMed:16581786, PubMed:16951159, PubMed:17908926, PubMed:17962192, PubMed:22464731, PubMed:22770219, PubMed:32267835). Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition (By similarity). Degradation of CDKN1B/p27kip also requires CKS1 (By similarity). Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, NBN, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1 (PubMed:11931757, PubMed:12435635, PubMed:12769844, PubMed:12840033, PubMed:15342634, PubMed:15668399, PubMed:15949444, PubMed:16103164, PubMed:16581786, PubMed:16951159, PubMed:17908926, PubMed:17962192, PubMed:22464731, PubMed:32267835). Degradation of TAL1 also requires STUB1 (PubMed:17962192). Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2 (PubMed:16262255). Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration (PubMed:22770219). Following phosphorylation in response to DNA damage, mediates 'Lys-63'-linked ubiquitination of NBN, promoting ATM recruitment to DNA damage sites and DNA repair via homologous recombination (PubMed:22464731). Through the ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A, has an antiviral activity towards that virus.

Alternative names

FBXL1, SKP2, S-phase kinase-associated protein 2, Cyclin-A/CDK2-associated protein p45, F-box protein Skp2, F-box/LRR-repeat protein 1, p45skp2

Recommended products

Rabbit Recombinant Monoclonal SKP2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 10 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3305(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

S-phase kinase-associated protein 2 (SKP2) also known as FBL1 or p45 is an E3 ubiquitin ligase that tags proteins for degradation. It weighs around 48 kDa. SKP2 facilitates the transition from the G1 to S phase of the cell cycle by targeting cyclin-dependent kinase inhibitors for proteasomal destruction. Expressed in various tissues including key roles in proliferating cells SKP2 ensures the regulation of cell cycle progression and stability.

Biological function summary

SKP2 is part of the SCF (SKP1-CUL1-F-box) complex which is instrumental in controlling cell cycle and cell division. It regulates the degradation of p27^Kip1 and other cell cycle regulators. By modulating proteolysis SKP2 contributes to the maintenance of cell proliferation and it has essential roles in cellular responses to growth signals. Moreover SKP2 influences processes such as DNA replication through its effect on substrate interaction and ubiquitination.

Pathways

SKP2 functions inside the ubiquitin-proteasome system and impacts the PI3K/AKT signaling pathway. SKP2 advances cell cycle progression by associating with proteins like p27^Kip1 and CDK2 facilitating cell division. It modulates the phosphorylation state of numerous targets influencing signaling processes that control cell growth. This involvement aligns SKP2 with key cellular pathways that maintain cell survival and metabolic balance.

Associated diseases and disorders

SKP2 plays an important role in cancer and cardiovascular diseases. In various cancers the aberrant expression of SKP2 connects to excessive cell proliferation and tumor growth partially through interactions with oncoproteins like Myc. In cardiovascular conditions SKP2 influences cardiac hypertrophy and might link to proteins such as cyclin-dependent kinase inhibitors. The regulation by SKP2 affects these proteins' stability impacting disease progression and potential therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-SKP2 antibody [EPR3305(2)] (ab183039), expandable thumbnail

    Western blot - Anti-SKP2 antibody [EPR3305(2)] (ab183039)

    Lanes 1 - 4: Merged signal (red and green). Green - ab183039 observed at 52 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab183039 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in SKP2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SKP2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab183039 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/200 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SKP2 antibody [EPR3305(2)] (ab183039) at 1/200 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: SKP2 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HeLa whole cell lysate at 20 µg

    Lane 4: MCF7 whole cell lysate at 20 µg

    Predicted band size: 45 kDa, 47 kDa, 55 kDa

    Observed band size: 45 kDa, 55 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SKP2 antibody [EPR3305(2)] (ab183039), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SKP2 antibody [EPR3305(2)] (ab183039)

    Immunofluorescenct analysis of 4% paraformaldehyde fixed 293T cells labeling SKP2 with ab183039 at 1/100 followed by Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 (green). Cells were counter stained with Dapi (blue).

  • Flow Cytometry (Intracellular) - Anti-SKP2 antibody [EPR3305(2)] (ab183039), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SKP2 antibody [EPR3305(2)] (ab183039)

    Intracellular flow cytometric analysis of 2% paraformaldehyde fixedHeLa cells labeling SKP2 with ab183039 at 1/70 followed byGoat anti rabbit IgG (FITC) at 1/150.Rabbit monoclonal IgG was used asIsotype control.

  • Western blot - Anti-SKP2 antibody [EPR3305(2)] (ab183039), expandable thumbnail

    Western blot - Anti-SKP2 antibody [EPR3305(2)] (ab183039)

    SKP2 Western blot staining using rabbit Anti-SKP2 antibody

    We recommend to increase the amount of samples or decrease antibody dilution to get clear bands.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SKP2 antibody [EPR3305(2)] (ab183039) at 1/200 dilution

    Lane 1: NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 2: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 3: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 4: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg

    Lane 5: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 6: A549 (Human lung carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 7: F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 8: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

    Lane 9: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKP2 antibody [EPR3305(2)] (ab183039), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKP2 antibody [EPR3305(2)] (ab183039)

    immunohistochemical analysis of paraffin embedded Human kidney clear cell carcinoma tissue labeling SKP2 with ab183039 at 1/50 followed by secondary staining with Ready to use HRP Polymer for Rabbit IgG and counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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