JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB267349

Anti-SLAMF6 antibody [EPR23122-130] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal SLAMF6 antibody. Carrier free. Suitable for IP, Flow Cyt, ICC/IF and reacts with Mouse samples.

View Alternative Names

CD352, Ly108, Slamf6, SLAM family member 6, Lymphocyte antigen 108

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR23122-130] - BSA and Azide free (AB267349)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR23122-130] - BSA and Azide free (AB267349)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse splenocytes cells labelling SLAMF6 with ab243658 at 1/50 dilution, followed by ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing staining in some subsets of mouse splenocytes is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243658).

Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR23122-130] - BSA and Azide free (AB267349)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR23122-130] - BSA and Azide free (AB267349)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling SLAMF6 with ab243658 at 1/50 dilution, followed by ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in EL4 cells is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243658).

Flow Cytometry - Anti-SLAMF6 antibody [EPR23122-130] - BSA and Azide free (AB267349)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-SLAMF6 antibody [EPR23122-130] - BSA and Azide free (AB267349)

Flow cytometric analysis of Mouse splenocytes cells labelling SLAMF6 with ab243658 at 1/500 compared with a Rabbit monoclonal IgG (ab172730, Left) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243658).

Immunoprecipitation - Anti-SLAMF6 antibody [EPR23122-130] - BSA and Azide free (AB267349)
  • IP

Unknown

Immunoprecipitation - Anti-SLAMF6 antibody [EPR23122-130] - BSA and Azide free (AB267349)

SLAMF6 was immunoprecipitated from 0.35 mg Mouse spleen lysate with ab243658 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243658 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1 : Mouse spleen lysate 10ug
Lane 2 : ab243658 IP in Mouse spleen lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab243658 in Mouse spleen lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 min

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243658).

All lanes:

Immunoprecipitation - Anti-SLAMF6 antibody [EPR23122-130] (<a href='/en-us/products/primary-antibodies/slamf6-antibody-epr23122-130-ab243658'>ab243658</a>)

Predicted band size: 37 kDa,87 kDa

false

  • Unconjugated

    Anti-SLAMF6 antibody [EPR23122-130]

  • 660 APC

    APC Anti-SLAMF6 antibody [EPR23122-130]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-SLAMF6 antibody [EPR23122-130]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-SLAMF6 antibody [EPR23122-130]

  • 578 PE

    PE Anti-SLAMF6 antibody [EPR23122-130]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23122-130

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

IP, ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB249206

Anti-SLAMF6 antibody [EPR9916] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-SLAMF6 antibody [EPR9916] - BSA and Azide free (AB249206)

  • 0
  • 0
Primary Antibodies

AB238421

Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (AB238421)

  • 0
  • 0
Primary Antibodies

AB245902

PE Anti-SLAMF6 antibody [EPR22170]

RabMAb|Recombinant

Flow Cytometry - PE Anti-SLAMF6 antibody [EPR22170] (AB245902)

  • 0
  • 0
Primary Antibodies

AB224201

Anti-SLAMF6 antibody [EPR22170]

20ul selling size|RabMAb|Recombinant|Trial Size

Flow Cytometry - Anti-SLAMF6 antibody [EPR22170] (AB224201)

  • 0
  • 0
Primary Antibodies

AB246707

Anti-SLAMF6 antibody [EPR22170] - Low endotoxin, Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-SLAMF6 antibody [EPR22170] - Low endotoxin, Azide free (AB246707)

  • 0
  • 0

Complementary Products

Assay Kits

AB277424

Human SLAMF6 ELISA Kit

ELISA - Human SLAMF6 ELISA Kit (AB277424)

  • 0
  • 0
Primary Antibodies

AB307651

Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Siglec 7 antibody [EPR26252-79] - BSA and Azide free (AB307651)

  • 0
  • 0
Primary Antibodies

AB272170

Anti-CD62L antibody [EPR23565-109] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD62L antibody [EPR23565-109] - BSA and Azide free (AB272170)

  • 0
  • 0
Primary Antibodies

AB302907

Anti-NKG2D antibody [EPR24072-342]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] (AB302907)

  • 0
  • 0
Primary Antibodies

AB224703

Anti-NCR1/NKp46 antibody [EPR22403-57]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1/NKp46 antibody [EPR22403-57] (AB224703)

  • 5
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

ab267349 is the carrier-free version of ab243658.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLAMF6 also known as CD352 is a protein that functions as a signaling lymphocytic activation molecule. It is a member of the SLAM family and is found on the surface of many immune cells including T cells B cells and natural killer (NK) cells. SLAMF6 has a molecular weight of approximately 71 kDa. This protein plays a role in cell-to-cell adhesion and transduction of activation signals. Its expression appears prominently in lymphoid tissues directing immune responses.
Biological function summary

SLAMF6 assists in modulating immune cell interactions and is part of specific receptor complexes. It influences the activation and proliferation of immune cells and contributes to the maintenance of immune homeostasis. SLAMF6 interacts with signaling pathways involving SAP (SLAM-associated protein) and EAT-2 which facilitate downstream signaling events essential for immune cell function and communication.

Pathways

SLAMF6 integrates within the SLAM receptor signaling pathway impacting the regulation of immune responses. It plays a role in the NF-kB signaling pathway important for inflammatory and immune responses. Related proteins in these pathways include SAP and EAT-2 important in signal transduction cascades that affect cell survival differentiation and function of immune cells.

SLAMF6 ties to autoimmune diseases and certain cancers. It is involved in autoimmune conditions such as systemic lupus erythematosus where malfunction in immune regulation occurs. Additionally deregulation of SLAMF6 expression associates with lymphomas impacting cell communication and immune function. In these conditions SLAMF6 interacts with SAP influencing the disease progression through altered immune signaling pathways.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2 (PubMed : 19648922). Triggers cytolytic activity only in natural killer cells (NK) expressing high surface densities of natural cytotoxicity receptors (By similarity). Positive signaling in NK cells implicates phosphorylation of VAV1. NK cell activation seems to depend on SH2D1B and not on SH2D1A (By similarity). In conjunction with SLAMF1 controls the transition between positive selection and the subsequent expansion and differentiation of the thymocytic natural killer T (NKT) cell lineage (PubMed : 18031695). Promotes T cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion; the costimulatory activity requires SH2D1A (By similarity). Promotes recruitment of RORC to the IL-17 promoter (By similarity). In conjunction with SLAMF1 and CD84/SLAMF5 may be a negative regulator of the humoral immune response (PubMed : 25926831). In the absence of SH2D1A/SAP can transmit negative signals to CD4(+) T-cells and NKT cells. Negatively regulates germinal center formation by inhibiting T-cell : B-cell adhesion; the function probably implicates increased association with PTPN6/SHP-1 via ITSMs in absence of SH2D1A/SAP (PubMed : 22683125). However, reported to mediated T-cell adhesion, to participate in stable T-cell : B-cell interactions and to be involved in maintaining B-cell tolerance in germinal centers and in preventing autoimmunity (PubMed : 20153220, PubMed : 25801429). Involved in regulation of autoimmunity. Isoform 3 may be suppressor of pathogenic T-cell proliferation (PubMed : 21422172).
See full target information Slamf6
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com