AB233779

Anti-SLAMF7/CS1 antibody [5G5A4]

Name: Anti-SLAMF7/CS1 antibody [5G5A4] (ab233779) | Abcam
Brand: Abcam Limited
SKU: AB233779
Price: 520 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal SLAMF7/CS1 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SLAMF7 aa 1-250.

View Alternative Names

CD319, CS1, UNQ576/PRO1138, SLAMF7, SLAM family member 7, CD2 subset 1, CD2-like receptor-activating cytotoxic cells, Membrane protein FOAP-12, Novel Ly9, Protein 19A, CRACC

5 Images

Flow Cytometry - Anti-SLAMF7/CS1 antibody [5G5A4] (AB233779)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-SLAMF7/CS1 antibody [5G5A4] (AB233779)

Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cells labeling SLAMF7/CS1 with ab233779 at 1/200 dilution (green) compared with a negative control (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [5G5A4] (AB233779)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [5G5A4] (AB233779)

Paraffin-embedded human tonsil tissue stained for SLAMF7/CS1 using ab233779 at 1/200 dilution in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [5G5A4] (AB233779)

Paraffin-embedded human rectum cancer tissue stained for SLAMF7/CS1 using ab233779 at 1/200 dilution in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-SLAMF7/CS1 antibody [5G5A4] (AB233779)

Expected MW is 48.3 kDa.

All lanes:

Western blot - Anti-SLAMF7/CS1 antibody [5G5A4] (ab233779) at 1/500 dilution

All lanes:

Recombinant human SLAMF7/CS1 (aa 23-226) protein

Predicted band size: 37 kDa

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Supplier Data

Western blot - Anti-SLAMF7/CS1 antibody [5G5A4] (AB233779)

All lanes:

Western blot - Anti-SLAMF7/CS1 antibody [5G5A4] (ab233779) at 1/500 dilution

Lane 1:

Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

Human SLAMF7/CS1 (aa 23-226)-hIgG-Fc-transfected HEK-293 cell lysate

Predicted band size: 37 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5G5A4

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human SLAMF7 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9NQ25

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLAMF7 also known as CS1 is a glycoprotein with a mass of approximately 70 kDa. This protein localizes on the surface of immune cells mainly on natural killer (NK) cells certain subsets of T cells and mature dendritic cells. It does not express in resting B cells but can be induced upon activation. CS1 plays a role in immune modulation by facilitating cell-to-cell interactions and activating various signaling pathways that enable immune responses.

Biological function summary

SLAMF7/CS1 modulates immune functions by engaging in interactions with other cell surface receptors. It often operates in the context of hematopoietic cells integrating signals that influence cell proliferation differentiation and survival. It does not require an associated adaptor molecule like most of the SLAM family members as it signals through its ability to recruit EAT-2. The CS1 protein modulates pathways related to immune cell activation and control making it an important player in the regulation of immune responses.

Pathways

CS1 participates prominently in immune signaling pathways where it interacts with proteins such as SAP and EAT-2. It is particularly involved in the immunoregulatory interactions contributing to NK cell-mediated cytotoxicity and modulation of T-cell responses. These interactions illustrate CS1's role in the modulation of immune responses positioning it within the broader network of immune signaling pathways important for maintaining immune homeostasis and defense mechanisms.

SLAMF7/CS1 shows key relevance in multiple myeloma and systemic lupus erythematosus (SLE). In multiple myeloma CS1 presents an overexpression and serves as a therapeutic target; its relationship with CD38 another important marker illustrates the potential for targeted therapies. In SLE improper signaling involving CS1 can contribute to the dysregulation of immune responses. Overall the intricate interactions of CS1 with other proteins highlight its significance in disease pathology and therapeutic potential.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway (PubMed : 11698418). Positively regulates NK cell functions by a mechanism dependent on phosphorylated SH2D1B. Downstream signaling implicates PLCG1, PLCG2 and PI3K (PubMed : 16339536). In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Also acts inhibitory in T-cells (By similarity). May play a role in lymphocyte adhesion (PubMed : 11802771). In LPS-activated monocytes negatively regulates production of pro-inflammatory cytokines (PubMed : 23695528).. Isoform 3 does not mediate any NK cell activation.

See full target information SLAMF7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & biomedicine 24:848-856 PubMed38149831

2023

Hypomethylation of the promoter region contributes to the occurrence and development of ovarian endometriosis via the AKT/mTOR/4EBP1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzhen Qin,Yishuai Li,Yali Hao,Yan Li,Shan Kang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLAMF7/CS1 antibody [5G5A4]

Flow Cytometry - Anti-SLAMF7/CS1 antibody [5G5A4] (AB233779)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [5G5A4] (AB233779)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [5G5A4] (AB233779)

Western blot - Anti-SLAMF7/CS1 antibody [5G5A4] (AB233779)

Western blot - Anti-SLAMF7/CS1 antibody [5G5A4] (AB233779)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise