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Rabbit Recombinant Monoclonal SLAMF7/CS1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (AB237730), expandable thumbnail
  • Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] (AB237730), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (AB237730), expandable thumbnail
  • Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] (AB237730), expandable thumbnail
  • Western blot - Anti-SLAMF7/CS1 antibody [CAL7] (AB237730), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.5
Preservative: 0.05% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Tested
Not recommended
Tested
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.1 µg/mL

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway (PubMed:11698418). Positively regulates NK cell functions by a mechanism dependent on phosphorylated SH2D1B. Downstream signaling implicates PLCG1, PLCG2 and PI3K (PubMed:16339536). In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells (By similarity). May play a role in lymphocyte adhesion (PubMed:11802771). In LPS-activated monocytes negatively regulates production of proinflammatory cytokines (PubMed:23695528).Isoform 3 does not mediate any NK cell activation.

Alternative names

SLAM family member 7, CD2 subset 1, CD2-like receptor-activating cytotoxic cells, Membrane protein FOAP-12, Novel Ly9, Protein 19A, CRACC, UNQ576/PRO1138, CS1, SLAMF7

Recommended products

Rabbit Recombinant Monoclonal SLAMF7/CS1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

Alternative names

SLAM family member 7, CD2 subset 1, CD2-like receptor-activating cytotoxic cells, Membrane protein FOAP-12, Novel Ly9, Protein 19A, CRACC, UNQ576/PRO1138, CS1, SLAMF7

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

CAL7

Purification technique

Affinity purification Protein A

Concentration
Loading...
Purification notes

Purity >99%

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

(aka clone 43H1L46)

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SLAMF7 also known as CS1 is a glycoprotein with a mass of approximately 70 kDa. This protein localizes on the surface of immune cells mainly on natural killer (NK) cells certain subsets of T cells and mature dendritic cells. It does not express in resting B cells but can be induced upon activation. CS1 plays a role in immune modulation by facilitating cell-to-cell interactions and activating various signaling pathways that enable immune responses.

Biological function summary

SLAMF7/CS1 modulates immune functions by engaging in interactions with other cell surface receptors. It often operates in the context of hematopoietic cells integrating signals that influence cell proliferation differentiation and survival. It does not require an associated adaptor molecule like most of the SLAM family members as it signals through its ability to recruit EAT-2. The CS1 protein modulates pathways related to immune cell activation and control making it an important player in the regulation of immune responses.

Pathways

CS1 participates prominently in immune signaling pathways where it interacts with proteins such as SAP and EAT-2. It is particularly involved in the immunoregulatory interactions contributing to NK cell-mediated cytotoxicity and modulation of T-cell responses. These interactions illustrate CS1's role in the modulation of immune responses positioning it within the broader network of immune signaling pathways important for maintaining immune homeostasis and defense mechanisms.

Associated diseases and disorders

SLAMF7/CS1 shows key relevance in multiple myeloma and systemic lupus erythematosus (SLE). In multiple myeloma CS1 presents an overexpression and serves as a therapeutic target; its relationship with CD38 another important marker illustrates the potential for targeted therapies. In SLE improper signaling involving CS1 can contribute to the dysregulation of immune responses. Overall the intricate interactions of CS1 with other proteins highlight its significance in disease pathology and therapeutic potential.

Product promise

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7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling SLAMF7/CS1 with ab237730 at 1/800 dilution for 15 minutes at room temperature, followed by ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human tonsil is observed. Counterstained with Hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730), expandable thumbnail

    Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)

    SLAMF7/CS1 was immunoprecipitated from 0.35mg of Human tonsil lysate with ab237730 at 1/30 dilution. Western blot was performed from the immunoprecipitated using ab237730 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as secondary antibody at 1/5000 dilution.

    Lane 1: Human tonsil lysate 10μg (Input)

    Lane 2: ab237730 IP in Human tonsil lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab237730 in Human tonsil lysate

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)

    Predicted band size: 37 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)

    Immunohistochemical analysis of paraffin-embedded human multiple myeloma tissue labelling SLAMF7/CS1 with ab237730 at 1/800 dilution for 15 minutes at room temperature, followed by ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly membranous staining on the human multiple myeloma is observed. Counterstained with Hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730), expandable thumbnail

    Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)

    SLAMF7/CS1 was immunoprecipitated from 0.35mg of IM-9 (human multiple myeloma B Lymphoblast) whole cell lysate with ab237730 at 1/30 dilution. Western blot was performed from the immunoprecipitated using ab237730 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as secondary antibody at 1/5000 dilution.

    Lane 1: IM-9 (human multiple myeloma B Lymphoblast) whole cell lysate 10μg (Input)

    Lane 2: ab237730 IP in IM-9 lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab237730 in IM-9 whole cell lysate

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    The expression profile observed is consistent with what has been described in the literature (PMID: 18451245; 11698418; 25312647), with the bands greater than 37 kDa predicted to be glycosylated SLAMF7/CS1.

    All lanes: Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)

    Predicted band size: 37 kDa

    Observed band size: 37 kDa

    Exposure time: 10s

  • Western blot - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730), expandable thumbnail

    Western blot - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time:
    Lane 1: 3 min
    Lane 2: 1min

    The expression profile observed is consistent with what has been described in the literature (PMID: 18451245; 11698418; 25312647), with the bands greater than 37 kDa predicted to be glycosylated SLAMF7/CS1.

    All lanes: Western blot - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730) at 1/1000 dilution

    All lanes: IM-9 (human multiple myeloma B Lymphoblast) whole cell lysate at 20 µg

    Secondary

    Lane 1: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Lane 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 37 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)

    Formalin-fixed, paraffin-embedded multiple myeloma (Lambda, left panel) and SLAMF7 Knock-in AD-293 cells (Positive control, right panel) stained for SLAMF7/CS1 using ab237730 at 0.1 μg/ml dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)

    Formalin-fixed, paraffin-embedded OPM2 xenograft (Postive control, left panel) and NCI-N87 xenograft (Negative control, right) tissue stained for SLAMF7/CS1 using ab237730 at 0.1 μg/ml dilution in immunohistochemical analysis.

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