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AB249590

Anti-SLAP2 antibody [EPR8083(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SLAP2 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C20orf156, SLAP2, SLA2, Src-like-adapter 2, Modulator of antigen receptor signaling, Src-like adapter protein 2, MARS, SLAP-2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-SLAP2 antibody [EPR8083(2)] - BSA and Azide free (AB249590)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SLAP2 antibody [EPR8083(2)] - BSA and Azide free (AB249590)

This data was developed using ab170918, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of Jurkat cells labeling SLAP2 with ab170918 at 1/100 (green). DAPI nuclear staining (blue).

Flow Cytometry (Intracellular) - Anti-SLAP2 antibody [EPR8083(2)] - BSA and Azide free (AB249590)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SLAP2 antibody [EPR8083(2)] - BSA and Azide free (AB249590)

This data was developed using ab170918, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling SLAP2 with ab170918 at 1/100 (red) or a rabbit IgG (negative) (green).

Immunoprecipitation - Anti-SLAP2 antibody [EPR8083(2)] - BSA and Azide free (AB249590)
  • IP

Supplier Data

Immunoprecipitation - Anti-SLAP2 antibody [EPR8083(2)] - BSA and Azide free (AB249590)

This data was developed using ab170918&

44; the same antibody clone in a different buffer formulation.

All lanes:

Immunoprecipitation - Anti-SLAP2 antibody [EPR8083(2)] (<a href='/en-us/products/primary-antibodies/slap2-antibody-epr80832-ab170918'>ab170918</a>) at 1/1000 dilution

Lane 1:

Immunoprecipitation pellet from Jurkat cell lysate using <a href='/en-us/products/primary-antibodies/slap2-antibody-epr80832-ab170918'>ab170918</a> at 1/10

Lane 2:

Immunoprecipitation pellet from 1X PBS using <a href='/en-us/products/primary-antibodies/slap2-antibody-epr80832-ab170918'>ab170918</a> at 1/10 (negative control)

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

Predicted band size: 29 kDa

true

Western blot - Anti-SLAP2 antibody [EPR8083(2)] - BSA and Azide free (AB249590)
  • WB

Supplier Data

Western blot - Anti-SLAP2 antibody [EPR8083(2)] - BSA and Azide free (AB249590)

This data was developed using ab170918, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SLAP2 antibody [EPR8083(2)] (<a href='/en-us/products/primary-antibodies/slap2-antibody-epr80832-ab170918'>ab170918</a>) at 1/1000 dilution

All lanes:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 29 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8083(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab249590 is the carrier-free version of ab170918.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Src-like adapter protein 2 (SLAP2) also known as SLAP2 functions as an adaptor protein involved in signal transduction. It mediates interactions between membrane receptors and intracellular signaling components. SLAP2 is a protein of approximately 32 kDa and expression levels vary across different tissues including high expression in immune cells such as lymphocytes and macrophages. The protein has SH2 and SH3 domains which allow it to bind to phosphorylated tyrosines on its target proteins facilitating their degradation or modification.
Biological function summary

SLAP2 has a major role in regulating the immune response. By forming complexes with the components of the signal transduction machinery SLAP2 modulates the activity of key receptors such as the T-cell receptor (TCR) and B-cell receptor (BCR). These interactions influence cellular responses such as proliferation differentiation and apoptosis. Through its action SLAP2 acts as a negative regulator preventing overactivation of immune responses and maintaining homeostasis.

Pathways

Studies have shown that SLAP2 participates in both the T-cell receptor signaling pathway and the B-cell receptor signaling pathway. In these pathways SLAP2 interacts with kinases associated with these receptors such as Lck and Fyn influencing downstream signaling events. SLAP2 can inhibit excessive activation of these pathways by promoting the degradation of key signaling molecules ensuring that cells respond appropriately to external signals during immune activation.

SLAP2 has been linked to certain types of cancer and autoimmune diseases. For example alterations in SLAP2 expression or function may contribute to lymphomas where disrupted signaling processes lead to uncontrolled cell growth. In autoimmune diseases aberrant SLAP2 activity could result in improper immune cell activation potentially linked to proteins like ZAP-70 which are also involved in disease modulation. Understanding SLAP2's regulation and interaction with signaling proteins could offer insights for therapeutic interventions in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins.
See full target information SLA2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bio-protocol 15:e5206 PubMed39959296

2025

Cell-Sonar, an Easy and Low-cost Method to Track a Target Protein by Expression Changes of Specific Protein Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Brockmöller,Lara Maria Molitor,Franz Worek,Simone Rothmiller
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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