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AB229677

Anti-SLBP antibody

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(1 Publication)

Rabbit Polyclonal SLBP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SLBP.

View Alternative Names

HBP, SLBP, Histone RNA hairpin-binding protein, Histone stem-loop-binding protein

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SLBP antibody (AB229677)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SLBP antibody (AB229677)

4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SLBP (green) using ab229677 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Western blot - Anti-SLBP antibody (AB229677)
  • WB

Supplier Data

Western blot - Anti-SLBP antibody (AB229677)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-SLBP antibody (ab229677) at 1/1000 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) cytoplasm extract at 30 µg

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SLBP. The exact immunogen used to generate this antibody is proprietary information.

Q14493

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Stem-loop binding protein (SLBP) also known as histone stem-loop binding protein is critical in the processing and regulation of histone mRNA. SLBP is a 31 kDa protein and it is found in the nucleus where it facilitates histone mRNA processing specifically the maturation and stabilization of histone pre-mRNA. This protein binds to the stem-loop structure found at the 3’ end of replication-dependent histone mRNA which is essential for proper histone production during the S phase of the cell cycle.
Biological function summary

Stem-loop binding protein interacts with histone pre-mRNA processing machinery. It is not part of any large complex but plays an important role in association with the 3' end processing mechanism. By binding the stem-loop structure SLBP participates in ensuring the stability of histone mRNA ultimately influencing histone protein synthesis. The regulation of these mRNAs is important for DNA packaging and chromosome stability which are critical processes for normal cell function and division.

Pathways

SLBP is involved in the histone mRNA processing pathway which is pivotal for eukaryotic cell replication. This pathway includes notable participants like the U7 small nuclear ribonucleoprotein (snRNP). SLBP influences the balance of histone proteins in relation to DNA synthesis. The correct function in this pathway maintains the balance between DNA replication and nucleosome assembly which is related to the cell cycle and genome integrity maintenance.

Irregularities in SLBP function or expression link to certain cancers such as breast cancer where abnormal histone mRNA processing can occur. It is also associated with developmental disorders due to its role in cell division and DNA replication. In cases of cancer SLBP interacts with proteins like the p53 pathway where disruptions can lead to uncontrolled cell proliferation and tumor growth.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein involved in the histone pre-mRNA processing (PubMed : 12588979, PubMed : 19155325, PubMed : 8957003, PubMed : 9049306). Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE) (PubMed : 12588979, PubMed : 19155325, PubMed : 8957003, PubMed : 9049306). Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery (PubMed : 12588979, PubMed : 19155325, PubMed : 8957003, PubMed : 9049306). Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression (PubMed : 12588979, PubMed : 19155325, PubMed : 8957003, PubMed : 9049306). Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis (By similarity). Binds to the 5' side of the stem-loop structure of histone pre-mRNAs (By similarity).
See full target information SLBP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 54:47-60 PubMed35031788

2022

Oncogenic E3 ubiquitin ligase NEDD4 binds to KLF8 and regulates the microRNA-132/NRF2 axis in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Minghuan Mao,Liang Yang,Jingyao Hu,Bing Liu,Xiling Zhang,Yili Liu,Ping Wang,Hangyu Li
View all publications
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