Anti-SLC10A1/NTCP1 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC10A1/NTCP1 antibody (AB131084)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SLC10A1/NTCP1 with ab131084 at 2 μg/ml. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with ab131084 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. DAB was used as the chromogen.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC10A1/NTCP1 antibody (AB131084)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling SLC10A1/NTCP1 with ab131084 at 2 μg/ml. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with ab131084 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. DAB was used as the chromogen.

• WB

Supplier Data

Western blot - Anti-SLC10A1/NTCP1 antibody (AB131084)

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with ab131084 at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1/5000 dilution for 1.5 hour at RT.

All lanes:

Western blot - Anti-SLC10A1/NTCP1 antibody (ab131084) at 0.5 µg/mL

Lane 1:

Human liver tissue lysate at 30 µg

Lane 2:

Rat liver tissue lysate at 30 µg

Lane 3:

Mouse liver tissue lysate at 30 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP secondary antibody at 1/5000 dilution

Observed band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-SLC10A1/NTCP1 antibody (AB131084)

The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.

The membrane was blocked with 5% non-fat milk/TBS for 1.5 hour at RT

All lanes:

Western blot - Anti-SLC10A1/NTCP1 antibody (ab131084) at 0.5 µg/mL

Lane 1:

Rat liver tissue lysates at 30 µg

Lane 2:

Rat RH35 whole cell lysates at 30 µg

Lane 3:

Rat kidney tissue lysates at 30 µg

Lane 4:

Rat brain tissue lysates at 30 µg

Lane 5:

Mouse liver tissue lysates at 30 µg

Lane 6:

Mouse HEPA1-6 whole cell lysates at 30 µg

Lane 7:

Mouse kidney tissue lysates at 30 µg

Lane 8:

Mouse brain tissue lysates at 30 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP secondary antibody at 1/5000 dilution

Predicted band size: 38 kDa

Observed band size: 50 kDa

false