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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal SLC10A1/NTCP1 antibody. Suitable for WB and reacts with Rat, Mouse, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | IHC-Fr | IHC-P | WB | |
---|---|---|---|---|
Human | Not recommended | Not recommended | Not recommended | Tested |
Mouse | Not recommended | Not recommended | Not recommended | Tested |
Rat | Not recommended | Not recommended | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium.(Microbial infection) Acts as a receptor for hepatitis B virus.
Sodium/bile acid cotransporter, Cell growth-inhibiting gene 29 protein, Na(+)/bile acid cotransporter, Na(+)/taurocholate transport protein, Sodium/taurocholate cotransporting polypeptide, Solute carrier family 10 member 1, GIG29, NTCP, SLC10A1
Rabbit Recombinant Monoclonal SLC10A1/NTCP1 antibody. Suitable for WB and reacts with Rat, Mouse, Human samples.
Sodium/bile acid cotransporter, Cell growth-inhibiting gene 29 protein, Na(+)/bile acid cotransporter, Na(+)/taurocholate transport protein, Sodium/taurocholate cotransporting polypeptide, Solute carrier family 10 member 1, GIG29, NTCP, SLC10A1
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR27105-63
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
NTCP1 is essential for hepatic bile acid uptake which is an important step in bile acid recycling and metabolism. It functions as a solitary transporter without forming complexes with other proteins. The protein intake bile salts and conjugates them with taurine and glycine. This process ensures the efficient cycling and detoxification of bile acids regulating their levels in the liver and bloodstream.
SLC10A1 also known as NTCP1 (Na+/taurocholate co-transporting polypeptide) is an important membrane transporter responsible for the uptake of bile acids. It has a molecular mass of approximately 38 kDa. This protein is predominantly expressed in the liver where it is located on the basolateral membrane of hepatocytes. By facilitating the transport of sodium ions and bile acids NTCP1 plays a significant role in maintaining bile acid homeostasis within the body.
The NTCP1 is critical in the enterohepatic circulation of bile acids a process tightly controlled within the body. It significantly influences pathways like the bile acid synthesis pathway where it works alongside other transporters such as ASBT (apical sodium-dependent bile acid transporter) and OSTα/OSTβ (organic solute transporter alpha/beta). Additionally NTCP1 is involved in the Farnesoid X Receptor (FXR) regulatory pathway which modulates bile acid synthesis regulating the expression of genes that encode for enzymes involved in the biosynthesis of bile acids.
Improper functioning of NTCP1 relates to liver diseases such as cholestasis. This condition results from impaired bile flow causing accumulation of bile acids that can damage liver cells. Another relevant disorder is hepatitis B infection. NTCP1 acts as a receptor for the hepatitis B virus mediating viral entry into hepatocytes. This interaction emphasizes its potential role in the development and progression of hepatitis B where proteins like hepatitis B surface antigens (HBsAg) engage with NTCP1 to facilitate the infection process.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer and concentration: 5% NFDM/TBSTNegative control: brain, cerebellum (Human Protein Atlas Database)
Exposure time:
All lanes: Western blot - Anti-SLC10A1/NTCP1 antibody [EPR27105-63] (AB307550) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate 20 μg
Lane 2: Mouse brain tissue lysate 20 μg
Lane 3: Rat liver tissue lysate 20 μg
Lane 4: Rat brain tissue lysate 20 μg
Lane 5: Human liver tissue lysate 20 μg
Lane 6: Human cerebellum tissue lysate 20 μg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Observed band size: 38 kDa
Exposure time: 15s
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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