Rabbit Recombinant Monoclonal SLC10A1/NTCP1 antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples.
Images
![Western blot - Anti-SLC10A1/NTCP1 antibody [EPR27105-63] - BSA and Azide free (AB307551), expandable thumbnail](https://content.abcam.com/products/images/slc10a1-ntcp1-antibody-epr27105-63-bsa-and-azide-free-ab307551--western-blot-img405684.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SLC10A1/NTCP1 antibody [EPR27105-63] - BSA and Azide free (AB307551)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC-Fr | IHC-P | WB | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
Target data
Function
As a major transporter of conjugated bile salts from plasma into the hepatocyte, it plays a key role in the enterohepatic circulation of bile salts necessary for the solubilization and absorption of dietary fat and fat-soluble vitamins (PubMed:14660639, PubMed:24867799, PubMed:34060352, PubMed:8132774). It is strictly dependent on the extracellular presence of sodium (PubMed:14660639, PubMed:24867799, PubMed:34060352, PubMed:8132774). It exhibits broad substrate specificity and transports various bile acids, such as taurocholate, cholate, as well as non-bile acid organic compounds, such as estrone sulfate (PubMed:14660639, PubMed:34060352). Works collaboratively with the ileal transporter (NTCP2), the organic solute transporter (OST), and the bile salt export pump (BSEP), to ensure efficacious biological recycling of bile acids during enterohepatic circulation (PubMed:33222321). (Microbial infection) Acts as a receptor for hepatitis B virus.
Alternative names
NTCP, GIG29, SLC10A1, Hepatic sodium/bile acid cotransporter, Cell growth-inhibiting gene 29 protein, Na(+)/bile acid cotransporter, Na(+)/taurocholate transport protein, Sodium/taurocholate cotransporting polypeptide, Solute carrier family 10 member 1
Recommended products
Rabbit Recombinant Monoclonal SLC10A1/NTCP1 antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27105-63
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLC10A1 also known as NTCP1 (Na+/taurocholate co-transporting polypeptide) is an important membrane transporter responsible for the uptake of bile acids. It has a molecular mass of approximately 38 kDa. This protein is predominantly expressed in the liver where it is located on the basolateral membrane of hepatocytes. By facilitating the transport of sodium ions and bile acids NTCP1 plays a significant role in maintaining bile acid homeostasis within the body.
- Biological function summary
NTCP1 is essential for hepatic bile acid uptake which is an important step in bile acid recycling and metabolism. It functions as a solitary transporter without forming complexes with other proteins. The protein intake bile salts and conjugates them with taurine and glycine. This process ensures the efficient cycling and detoxification of bile acids regulating their levels in the liver and bloodstream.
- Pathways
The NTCP1 is critical in the enterohepatic circulation of bile acids a process tightly controlled within the body. It significantly influences pathways like the bile acid synthesis pathway where it works alongside other transporters such as ASBT (apical sodium-dependent bile acid transporter) and OSTα/OSTβ (organic solute transporter alpha/beta). Additionally NTCP1 is involved in the Farnesoid X Receptor (FXR) regulatory pathway which modulates bile acid synthesis regulating the expression of genes that encode for enzymes involved in the biosynthesis of bile acids.
- Associated diseases and disorders
Improper functioning of NTCP1 relates to liver diseases such as cholestasis. This condition results from impaired bile flow causing accumulation of bile acids that can damage liver cells. Another relevant disorder is hepatitis B infection. NTCP1 acts as a receptor for the hepatitis B virus mediating viral entry into hepatocytes. This interaction emphasizes its potential role in the development and progression of hepatitis B where proteins like hepatitis B surface antigens (HBsAg) engage with NTCP1 to facilitate the infection process.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-SLC10A1/NTCP1 antibody [EPR27105-63] - BSA and Azide free (ab307551), expandable thumbnail](https://content.abcam.com/products/images/slc10a1-ntcp1-antibody-epr27105-63-bsa-and-azide-free-ab307551--western-blot-img405684.jpg "Western blot - Anti-SLC10A1/NTCP1 antibody [EPR27105-63] - BSA and Azide free (ab307551)")
Western blot - Anti-SLC10A1/NTCP1 antibody [EPR27105-63] - BSA and Azide free (ab307551)
This data was developed using 307550, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Negative control: brain, cerebellum (Human Protein Atlas Database)
Exposure time:All lanes: Western blot - Anti-SLC10A1/NTCP1 antibody [EPR27105-63] (Anti-SLC10A1/NTCP1 antibody [EPR27105-63] ab307550) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate 20 μg
Lane 2: Mouse brain tissue lysate 20 μg
Lane 3: Rat liver tissue lysate 20 μg
Lane 4: Rat brain tissue lysate 20 μg
Lane 5: Human liver tissue lysate 20 μg
Lane 6: Human cerebellum tissue lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 38 kDa
Exposure time: 15s
Downloads
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