AB171747

Anti-SLC12A1/NKCC2 antibody [EPR11842]

RabMAb
Recombinant
What is this?

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(7 Publications )

Rabbit Recombinant Monoclonal SLC12A1/NKCC2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.

View Alternative Names

NKCC2, SLC12A1, Solute carrier family 12 member 1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, Kidney-specific Na-K-Cl symporter, Na-K-2Cl cotransporter 2, BSC1

2 Images

Flow Cytometry (Intracellular) - Anti-SLC12A1/NKCC2 antibody [EPR11842] (AB171747)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SLC12A1/NKCC2 antibody [EPR11842] (AB171747)

Intracellular flow cytometric analysis of permeabilized K562 cells labeling SLC12A1/NKCC2 with ab171747 at 1/100 dilution (red), compared with a rabbit IgG (negative) (green).

Supplier Data

Western blot - Anti-SLC12A1/NKCC2 antibody [EPR11842] (AB171747)

All lanes:

Western blot - Anti-SLC12A1/NKCC2 antibody [EPR11842] (ab171747) at 1/1000 dilution

Lane 1:

293T cell lysates at 10 µg

Lane 2:

Human fetal kidney lysates at 10 µg

Lane 3:

K562 cell lysates at 10 µg

Lane 4:

WI-38 cell lysate at 10 µg

Predicted band size: 121 kDa

false

Carrier free

Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11842

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The homology between SLC12A1/NKCC2 and SLC12A2 is 67%, so ab171747 may cross react with SLC12A2. We have not performed any laboratory testing to validate this.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SLC12A1 protein also known as the NKCC2 transporter is an important player in ion transport within renal physiology. With a molecular weight of around 120 kDa NKCC2 functions in the reabsorption of sodium potassium and chloride ions a function facilitated by its location in the thick ascending limb of the loop of Henle in the kidneys. This location and functionality are critical for renal concentration mechanisms and contribute to the maintenance of electrolyte and fluid balance.

Biological function summary

This transporter plays an essential role in concentrating urine and regulating blood pressure. NKCC2 is a part of the larger solute carrier family and does not usually form complexes with other proteins. It facilitates the trans-epithelial movement of Na+ K+ and 2Cl- ions from the lumen into renal epithelial cells which is fundamental for the creation of an osmotic gradient essential for water reabsorption.

Pathways

The SLC12A1 protein is a significant component of renal electrolyte transport and blood pressure regulation pathways. It is intricately involved in the renin-angiotensin-aldosterone system (RAAS) which regulates blood volume and systemic vascular resistance. In these pathways NKCC2's activity tightly connects it to other ion channel proteins and transporters such as ENaC which also play roles in blood pressure regulation by influencing sodium handling in the nephron.

Dysregulation of NKCC2 function can result in Bartter syndrome and hypertension. Bartter syndrome a rare disorder characterized by salt wasting and electrolyte imbalances stems from mutations affecting NKCC2. This transporter also impacts hypertension where its dysregulation can alter sodium reabsorption processes linking it to other proteins involved in blood pressure control such as the mineralocorticoid receptor. Understanding the role of NKCC2 provides insight into potential therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Renal sodium, potassium and chloride ion cotransporter that mediates the transepithelial NaCl reabsorption in the thick ascending limb and plays an essential role in the urinary concentration and volume regulation (PubMed : 21321328). Electrically silent transporter system (By similarity).

See full target information SLC12A1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 20:2475-2484 PubMed40027875

2025

Isolating Astrocyte-Derived Extracellular Vesicles From Urine.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Hui Xie,Mian-Mian Chen,Shu-Xian Xu,Junhua Mei,Qing Yang,Chao Wang,Honggang Lyu,Qian Gong,Zhongchun Liu

Cell systems 15:649-661.e9 PubMed38981488

2024

Highly parallel production of designer organoids by mosaic patterning of progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine M Porter,Grace C Qian,Samuel H Grindel,Alex J Hughes

Cell reports 33:108514 PubMed33326782

2020

Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling.

Applications

Unspecified application

Species

Unspecified reactive species

Kohei Uchimura,Haojia Wu,Yasuhiro Yoshimura,Benjamin D Humphreys

Hypertension (Dallas, Tex. : 1979) 73:112-120 PubMed30571558

2018

Norepinephrine-Induced Stimulation of Kir4.1/Kir5.1 Is Required for the Activation of NaCl Transporter in Distal Convoluted Tubule.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Peng Duan,Li Gu,Yu Xiao,Zhong-Xiuzi Gao,Peng Wu,Yun-Hong Zhang,Xin-Xin Meng,Jun-Lin Wang,Dan-Dan Zhang,Dao-Hong Lin,Wen-Hui Wang,Ruimin Gu

BMC genomics 17:724 PubMed27613513

2016

A missense mutation in solute carrier family 12, member 1 (SLC12A1) causes hydrallantois in Japanese Black cattle.

Applications

Unspecified application

Species

Cow

Shinji Sasaki,Kiyotoshi Hasegawa,Tomoko Higashi,Yutaka Suzuki,Sumio Sugano,Yasuaki Yasuda,Yoshikazu Sugimoto

Oncotarget 7:53571-53582 PubMed27447551

2016

Treatment with an SLC12A1 antagonist inhibits tumorigenesis in a subset of hepatocellular carcinomas.

Applications

IHC

Species

Unspecified reactive species

Fei Teng,Meng Guo,Fang Liu,Ce Wang,Jiayong Dong,Lei Zhang,You Zou,Rui Chen,Keyan Sun,Hong Fu,Zhiren Fu,Wenyuan Guo,Guoshan Ding

Nature communications 6:8835 PubMed26617239

2015

Endothelial Gata5 transcription factor regulates blood pressure.

Applications

Species

Mouse

Smail Messaoudi,Ying He,Alex Gutsol,Andrew Wight,Richard L Hébert,Ragnar O Vilmundarson,Andrew P Makrigiannis,John Chalmers,Pavel Hamet,Johanne Tremblay,Ruth McPherson,Alexandre F R Stewart,Rhian M Touyz,Mona Nemer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLC12A1/NKCC2 antibody [EPR11842]

Flow Cytometry (Intracellular) - Anti-SLC12A1/NKCC2 antibody [EPR11842] (AB171747)

Western blot - Anti-SLC12A1/NKCC2 antibody [EPR11842] (AB171747)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise