JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB213653

Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal SLC12A1/NKCC2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NKCC2, SLC12A1, Solute carrier family 12 member 1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, Kidney-specific Na-K-Cl symporter, Na-K-2Cl cotransporter 2, BSC1

2 Images
Flow Cytometry (Intracellular) - Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free (AB213653)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free (AB213653)

This data was developed using ab171747, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized K562 cells labeling SLC12A1/NKCC2 with ab171747 at 1/100 dilution (red), compared with a rabbit IgG (negative) (green).

Western blot - Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free (AB213653)
  • WB

Supplier Data

Western blot - Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free (AB213653)

This data was developed using ab171747, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SLC12A1/NKCC2 antibody [EPR11842] (<a href='/en-us/products/primary-antibodies/slc12a1-nkcc2-antibody-epr11842-ab171747'>ab171747</a>) at 1/1000 dilution

Lane 1:

293T cell lysates at 10 µg

Lane 2:

Human fetal kidney lysates at 10 µg

Lane 3:

K562 cell lysates at 10 µg

Lane 4:

WI-38 cell lysate at 10 µg

Predicted band size: 121 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11842

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The homology between SLC12A1/NKCC2 and SLC12A2 is 67%, so ab213653 may cross react with SLC12A2. We have not performed any laboratory testing to validate this.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

Product details

ab213653 is the carrier-free version of ab171747.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SLC12A1 protein also known as the NKCC2 transporter is an important player in ion transport within renal physiology. With a molecular weight of around 120 kDa NKCC2 functions in the reabsorption of sodium potassium and chloride ions a function facilitated by its location in the thick ascending limb of the loop of Henle in the kidneys. This location and functionality are critical for renal concentration mechanisms and contribute to the maintenance of electrolyte and fluid balance.
Biological function summary

This transporter plays an essential role in concentrating urine and regulating blood pressure. NKCC2 is a part of the larger solute carrier family and does not usually form complexes with other proteins. It facilitates the trans-epithelial movement of Na+ K+ and 2Cl- ions from the lumen into renal epithelial cells which is fundamental for the creation of an osmotic gradient essential for water reabsorption.

Pathways

The SLC12A1 protein is a significant component of renal electrolyte transport and blood pressure regulation pathways. It is intricately involved in the renin-angiotensin-aldosterone system (RAAS) which regulates blood volume and systemic vascular resistance. In these pathways NKCC2's activity tightly connects it to other ion channel proteins and transporters such as ENaC which also play roles in blood pressure regulation by influencing sodium handling in the nephron.

Dysregulation of NKCC2 function can result in Bartter syndrome and hypertension. Bartter syndrome a rare disorder characterized by salt wasting and electrolyte imbalances stems from mutations affecting NKCC2. This transporter also impacts hypertension where its dysregulation can alter sodium reabsorption processes linking it to other proteins involved in blood pressure control such as the mineralocorticoid receptor. Understanding the role of NKCC2 provides insight into potential therapeutic targets for these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Renal sodium, potassium and chloride ion cotransporter that mediates the transepithelial NaCl reabsorption in the thick ascending limb and plays an essential role in the urinary concentration and volume regulation (PubMed : 21321328). Electrically silent transporter system (By similarity).
See full target information SLC12A1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 21: PubMed32197418

2020

Adrenoceptor Expression during Intervertebral Disc Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Kupka,Annika Kohler,Karima El Bagdadi,Richard Bostelmann,Marco Brenneis,Christoph Fleege,Danny Chan,Frank Zaucke,Andrea Meurer,Marcus Rickert,Zsuzsa Jenei-Lanzl
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB199975

Anti-Aquaporin 2 antibody [EPR21080]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 antibody [EPR21080] (AB199975)

  • 5
  • 2
Primary Antibodies

AB316191

Anti-SLC12A3 antibody [EPR27106-48]

BOND RX™ Validated|20ul selling size|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (AB316191)

  • 0
  • 0
Primary Antibodies

AB81283

Anti-AKT1 (phospho S473) antibody [EP2109Y]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody [EP2109Y] (AB81283)

  • 4
  • 10
Primary Antibodies

AB77995

Anti-MUC5B antibody [19.4E]

Immunohistochemistry (Frozen sections) - Anti-MUC5B antibody [19.4E] (AB77995)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com