AB191315

Anti-SLC12A1/NKCC2 antibody - N-terminal

Name: Anti-SLC12A1/NKCC2 antibody - N-terminal (ab191315) | Abcam
Brand: Abcam Limited
SKU: AB191315
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal SLC12A1/NKCC2 antibody. N-terminal. Suitable for IHC-P and reacts with Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SLC12A1 aa 100-150.

View Alternative Names

NKCC2, SLC12A1, Solute carrier family 12 member 1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, Kidney-specific Na-K-Cl symporter, Na-K-2Cl cotransporter 2, BSC1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody - N-terminal (AB191315)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody - N-terminal (AB191315)

Immunohistochemical analysis of paraffin-embedded section of rat kidney tissue, labelling SLC12A1/NKCC2 with ab191315 at 2 μg/ml.

Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody - N-terminal (AB191315)

Immunohistochemical analysis of paraffin-embedded section of mouse kidney tissue, labelling SLC12A1/NKCC2 with ab191315 at 2 μg/ml.

Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human SLC12A1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q13621

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Constituents: 76.92% Trehalose, 17.31% Sodium chloride, 3.85% Disodium hydrogenorthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SLC12A1 protein also known as the NKCC2 transporter is an important player in ion transport within renal physiology. With a molecular weight of around 120 kDa NKCC2 functions in the reabsorption of sodium potassium and chloride ions a function facilitated by its location in the thick ascending limb of the loop of Henle in the kidneys. This location and functionality are critical for renal concentration mechanisms and contribute to the maintenance of electrolyte and fluid balance.

Biological function summary

This transporter plays an essential role in concentrating urine and regulating blood pressure. NKCC2 is a part of the larger solute carrier family and does not usually form complexes with other proteins. It facilitates the trans-epithelial movement of Na+ K+ and 2Cl- ions from the lumen into renal epithelial cells which is fundamental for the creation of an osmotic gradient essential for water reabsorption.

Pathways

The SLC12A1 protein is a significant component of renal electrolyte transport and blood pressure regulation pathways. It is intricately involved in the renin-angiotensin-aldosterone system (RAAS) which regulates blood volume and systemic vascular resistance. In these pathways NKCC2's activity tightly connects it to other ion channel proteins and transporters such as ENaC which also play roles in blood pressure regulation by influencing sodium handling in the nephron.

Dysregulation of NKCC2 function can result in Bartter syndrome and hypertension. Bartter syndrome a rare disorder characterized by salt wasting and electrolyte imbalances stems from mutations affecting NKCC2. This transporter also impacts hypertension where its dysregulation can alter sodium reabsorption processes linking it to other proteins involved in blood pressure control such as the mineralocorticoid receptor. Understanding the role of NKCC2 provides insight into potential therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Renal sodium, potassium and chloride ion cotransporter that mediates the transepithelial NaCl reabsorption in the thick ascending limb and plays an essential role in the urinary concentration and volume regulation (PubMed : 21321328). Electrically silent transporter system (By similarity).

See full target information SLC12A1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Clinical and experimental nephrology 29:393-404 PubMed39666151

2024

Nephrotic syndrome induces the upregulation of cell proliferation-related genes in tubular cells in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuya Suzuki,Ryohei Kaseda,Yusuke Nakagawa,Hirofumi Watanabe,Tadashi Otsuka,Suguru Yamamoto,Yoshikatsu Kaneko,Shin Goto,Taiji Matsusaka,Ichiei Narita

FASEB journal : official publication of the Federa 33:5887-5902 PubMed30721632

2019

IL-6/Smad2 signaling mediates acute kidney injury and regeneration in a murine model of neonatal hyperoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmine Mohr,Jenny Voggel,Christina Vohlen,Katharina Dinger,Claudia Dafinger,Gregor Fink,Heike Göbel,Max C Liebau,Jörg Dötsch,Miguel A Alejandre Alcazar

Dose-response : a publication of International Hormesis Society 16:1559325818811522 PubMed30479587

2018

Sodium Dichloroacetate Pharmacological Effect as Related to Na-K-2Cl Cotransporter Inhibition in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jūratė Stanevičiūtė,Milda Juknevičienė,Jolita Palubinskienė,Ingrida Balnytė,Angelija Valančiūtė,Rūta Vosyliūtė,Kęstutis Sužiedėlis,Vaiva Lesauskaitė,Donatas Stakišaitis

BMC genomics 17:724 PubMed27613513

2016

A missense mutation in solute carrier family 12, member 1 (SLC12A1) causes hydrallantois in Japanese Black cattle.

Applications

IHC-Fr

Species

Cow

Shinji Sasaki,Kiyotoshi Hasegawa,Tomoko Higashi,Yutaka Suzuki,Sumio Sugano,Yasuaki Yasuda,Yoshikazu Sugimoto

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLC12A1/NKCC2 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody - N-terminal (AB191315)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody - N-terminal (AB191315)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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