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AB224762

Anti-SLC12A3 antibody

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(4 Publications)

Rabbit Polyclonal SLC12A3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC12A3 aa 800-1000.

View Alternative Names

NCC, TSC, SLC12A3, Solute carrier family 12 member 3, Na-Cl cotransporter, Na-Cl symporter, Thiazide-sensitive sodium-chloride cotransporter

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB224762)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB224762)

Paraffin-embedded human kidney tissue stained for SLC12A3 using ab224762 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SLC12A3 aa 800-1000. The exact immunogen used to generate this antibody is proprietary information.

P55017

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SLC12A3 gene encodes the thiazide-sensitive sodium-chloride co-transporter (NCC) also known as solute carrier family 12 member 3. This protein plays an important role in sodium and chloride ion transport across cell membranes in the distal convoluted tubule of the nephron in the kidney influencing electrolyte and fluid balance. It is a membrane protein with a mass of about 108 kDa. SLC12A3 is highly expressed in the kidney specifically localized in the distal convoluted tubule where it contributes to ions balance.
Biological function summary

The activity of SLC12A3 regulates blood pressure and volume homeostasis. It is an integral part of ion transport mechanisms and operates within a network of other transporters and channels in the kidney. The proper function of this transporter balances sodium and chloride reabsorption preventing excessive loss or retention. While not directly forming a complex its activity correlates with the function of other ion transport proteins in maintaining ion balance.

Pathways

The SLC12A3 protein integrates into the renal system's regulation of electrolyte homeostasis. It interacts within the salt-sensitive pathway by influencing the reabsorption of sodium chloride which impacts the renin-angiotensin-aldosterone system (RAAS) indirectly. The activity of SLC12A3 influences blood pressure regulation connecting it functionally with proteins like WNK kinases which modulate its phosphorylation state altering its transport capacity.

SLC12A3 mutations lead to Gitelman syndrome a kidney disorder causing hypokalemic alkalosis hypomagnesemia and low blood pressure. Individuals with this disorder have impaired function of the SLC12A3 protein resulting in improper sodium and chloride reabsorption. The disorder connects with the renin-angiotensin pathway which tries to compensate by increasing renin production. It contrasts with conditions involving the CFTR protein which affects ion transport in diseases such as cystic fibrosis but focuses on chloride channels rather than co-transporters.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of sodium and chloride reabsorption in kidney distal convoluted tubules (PubMed : 18270262, PubMed : 21613606, PubMed : 22009145, PubMed : 36351028, PubMed : 36792826). Also acts as a receptor for the pro-inflammatory cytokine IL18, thereby contributing to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2 (By similarity). May act either independently of IL18R1, or in a complex with IL18R1 (By similarity).
See full target information SLC12A3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 239:e31437 PubMed39300779

2024

KDF1 promotes ameloblast differentiation by inhibiting the IKK/IκB/NF-κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hangbo Liu,Miao Yu,Kai Sun,Jinglei Zheng,Jiayu Wang,Haochen Liu,Hailan Feng,Yang Liu,Dong Han

International journal of molecular sciences 24: PubMed36769335

2023

CRISPR-Cas9-Mediated Correction of Gene Mutation Rescues the Gitelman's Disease Phenotype in a Patient-Derived Kidney Organoid System.

Applications

Unspecified application

Species

Unspecified reactive species

Sun Woo Lim,Xianying Fang,Sheng Cui,Hanbi Lee,Yoo Jin Shin,Eun Jeong Ko,Kang In Lee,Jae Young Lee,Byung Ha Chung,Chul Woo Yang

Diabetic medicine : a journal of the British Diabe 37:1879-1889 PubMed32634861

2020

The single nucleotide polymorphism rs11643718 in SLC12A3 is associated with the development of diabetic kidney disease in Chinese people with type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

J-F Yang,X-F Xiong,Y Xiao,L Wei,L Li,M Yang,Y-C Han,H Zhao,C-R Li,N Jiang,S Xiong,L-F Zeng,Z-G Zhou,S-P Liu,N-S Wang,Y Fan,L Sun

JCI insight 5: PubMed32255763

2020

Intercalated cell BKα subunit is required for flow-induced K+ secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Rolando Carrisoza-Gaytan,Evan C Ray,Daniel Flores,Allison L Marciszyn,Peng Wu,Leah Liu,Arohan R Subramanya,WenHui Wang,Shaohu Sheng,Lubika J Nkashama,Jingxin Chen,Edwin K Jackson,Stephanie M Mutchler,Szilvia Heja,Donald E Kohan,Lisa M Satlin,Thomas R Kleyman
View all publications
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Product promise

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