AB233401

Anti-SLC12A3 antibody

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(1 Publication)

Rabbit Polyclonal SLC12A3 antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human, Pig samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SLC12A3 aa 1-150.

View Alternative Names

NCC, TSC, SLC12A3, Solute carrier family 12 member 3, Na-Cl cotransporter, Na-Cl symporter, Thiazide-sensitive sodium-chloride cotransporter

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB233401)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB233401)

Formalin-fixed, paraffin-embedded human glioma tissue stained for SLC12A3 using ab233401 at 10 μg/mL in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB233401)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB233401)

Formalin-fixed, paraffin-embedded human stomach tissue stained for SLC12A3 using ab233401 at 10 μg/mL in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB233401)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB233401)

Formalin-fixed, paraffin-embedded human liver tissue stained for SLC12A3 using ab233401 at 10 μg/mL in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB233401)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB233401)

Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for SLC12A3 using ab233401 at 10 μg/mL in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB233401)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB233401)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for SLC12A3 using ab233401 at 10 μg/mL in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB233401)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB233401)

Formalin-fixed, paraffin-embedded human kidney tissue stained for SLC12A3 using ab233401 at 10 μg/mL in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

Western blot - Anti-SLC12A3 antibody (AB233401)

WB

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Western blot - Anti-SLC12A3 antibody (AB233401)

All lanes:

Western blot - Anti-SLC12A3 antibody (ab233401) at 1 µg/mL

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

Pig kidney tissue lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution

Predicted band size: 113 kDa

false

Western blot - Anti-SLC12A3 antibody (AB233401)

WB

Supplier Data

Western blot - Anti-SLC12A3 antibody (AB233401)

All lanes:

Western blot - Anti-SLC12A3 antibody (ab233401) at 2 µg/mL

All lanes:

Recombinant human SLC12A3 protein

Predicted band size: 113 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

WB, IHC-P

Immunogen

Recombinant Fragment Protein within Human SLC12A3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SLC12A3 gene encodes the thiazide-sensitive sodium-chloride co-transporter (NCC) also known as solute carrier family 12 member 3. This protein plays an important role in sodium and chloride ion transport across cell membranes in the distal convoluted tubule of the nephron in the kidney influencing electrolyte and fluid balance. It is a membrane protein with a mass of about 108 kDa. SLC12A3 is highly expressed in the kidney specifically localized in the distal convoluted tubule where it contributes to ions balance.

Biological function summary

The activity of SLC12A3 regulates blood pressure and volume homeostasis. It is an integral part of ion transport mechanisms and operates within a network of other transporters and channels in the kidney. The proper function of this transporter balances sodium and chloride reabsorption preventing excessive loss or retention. While not directly forming a complex its activity correlates with the function of other ion transport proteins in maintaining ion balance.

Pathways

The SLC12A3 protein integrates into the renal system's regulation of electrolyte homeostasis. It interacts within the salt-sensitive pathway by influencing the reabsorption of sodium chloride which impacts the renin-angiotensin-aldosterone system (RAAS) indirectly. The activity of SLC12A3 influences blood pressure regulation connecting it functionally with proteins like WNK kinases which modulate its phosphorylation state altering its transport capacity.

SLC12A3 mutations lead to Gitelman syndrome a kidney disorder causing hypokalemic alkalosis hypomagnesemia and low blood pressure. Individuals with this disorder have impaired function of the SLC12A3 protein resulting in improper sodium and chloride reabsorption. The disorder connects with the renin-angiotensin pathway which tries to compensate by increasing renin production. It contrasts with conditions involving the CFTR protein which affects ion transport in diseases such as cystic fibrosis but focuses on chloride channels rather than co-transporters.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of sodium and chloride reabsorption in kidney distal convoluted tubules (PubMed : 18270262, PubMed : 21613606, PubMed : 22009145, PubMed : 36351028, PubMed : 36792826). Also acts as a receptor for the pro-inflammatory cytokine IL18, thereby contributing to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2 (By similarity). May act either independently of IL18R1, or in a complex with IL18R1 (By similarity).

See full target information SLC12A3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35008625

2021

Regulation and Function of Laminin A5 during Mouse and Human Decidualization.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen-Shan Yang,Hai-Yang Pan,Wen-Wen Shi,Si-Ting Chen,Ying Wang,Meng-Yuan Li,Hai-Yi Zhang,Chen Yang,Ai-Xia Liu,Zeng-Ming Yang

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Product promise

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