AB95302

Anti-SLC12A3 antibody

Name: Anti-SLC12A3 antibody (ab95302) | Abcam
Brand: Abcam Limited
SKU: AB95302
Price: 430 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(16 Publications)

Rabbit Polyclonal SLC12A3 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Rat Slc12a3 aa 50-100.

View Alternative Names

NCC, TSC, SLC12A3, Solute carrier family 12 member 3, Na-Cl cotransporter, Na-Cl symporter, Thiazide-sensitive sodium-chloride cotransporter

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB95302)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB95302)

Immunofluorescent analysis of SLC12A3 in Rat kidney tissues using ab95302 at 1 : 200 dilution. DAPI merge.

Unknown

Western blot - Anti-SLC12A3 antibody (AB95302)

Western blot detection of SLC12A3 in Rat tissues using ab95302 at 1 : 1000 dilution.

All lanes:

Western blot - Anti-SLC12A3 antibody (ab95302)

Predicted band size: 113 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Mouse

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Rat Slc12a3 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P55018

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.007% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SLC12A3 gene encodes the thiazide-sensitive sodium-chloride co-transporter (NCC) also known as solute carrier family 12 member 3. This protein plays an important role in sodium and chloride ion transport across cell membranes in the distal convoluted tubule of the nephron in the kidney influencing electrolyte and fluid balance. It is a membrane protein with a mass of about 108 kDa. SLC12A3 is highly expressed in the kidney specifically localized in the distal convoluted tubule where it contributes to ions balance.

Biological function summary

The activity of SLC12A3 regulates blood pressure and volume homeostasis. It is an integral part of ion transport mechanisms and operates within a network of other transporters and channels in the kidney. The proper function of this transporter balances sodium and chloride reabsorption preventing excessive loss or retention. While not directly forming a complex its activity correlates with the function of other ion transport proteins in maintaining ion balance.

Pathways

The SLC12A3 protein integrates into the renal system's regulation of electrolyte homeostasis. It interacts within the salt-sensitive pathway by influencing the reabsorption of sodium chloride which impacts the renin-angiotensin-aldosterone system (RAAS) indirectly. The activity of SLC12A3 influences blood pressure regulation connecting it functionally with proteins like WNK kinases which modulate its phosphorylation state altering its transport capacity.

SLC12A3 mutations lead to Gitelman syndrome a kidney disorder causing hypokalemic alkalosis hypomagnesemia and low blood pressure. Individuals with this disorder have impaired function of the SLC12A3 protein resulting in improper sodium and chloride reabsorption. The disorder connects with the renin-angiotensin pathway which tries to compensate by increasing renin production. It contrasts with conditions involving the CFTR protein which affects ion transport in diseases such as cystic fibrosis but focuses on chloride channels rather than co-transporters.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of sodium and chloride reabsorption in kidney distal convoluted tubules (PubMed : 18270262, PubMed : 21613606, PubMed : 22009145, PubMed : 36351028, PubMed : 36792826). Also acts as a receptor for the pro-inflammatory cytokine IL18, thereby contributing to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2 (By similarity). May act either independently of IL18R1, or in a complex with IL18R1 (By similarity).

See full target information SLC12A3

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2531 PubMed40087269

2025

Expression of ENL YEATS domain tumor mutations in nephrogenic or stromal lineage impairs kidney development.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoyu Xue,Hongwen Xuan,Kin Lau,Yangzhou Su,Marc Wegener,Kuai Li,Lisa Turner,Marie Adams,Xiaobing Shi,Hong Wen

International journal of nanomedicine 20:2475-2484 PubMed40027875

2025

Isolating Astrocyte-Derived Extracellular Vesicles From Urine.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Hui Xie,Mian-Mian Chen,Shu-Xian Xu,Junhua Mei,Qing Yang,Chao Wang,Honggang Lyu,Qian Gong,Zhongchun Liu

Scientific reports 15:915 PubMed39762506

2025

Renal glomerular and tubular injury in the offspring of the preeclampsia-like syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Wang,Hao Wang,Huiqing Lu,Ji Ma,Wei Wu,Yinan Wang,Bo Ma,Hao Zhu,Rong Hu

Frontiers in cell and developmental biology 12:1480217 PubMed39479517

2024

Extra-cerebral recombination activity of Emx1-Cre and nestin-Cre in the kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wang,Xiaojuan Liu,Yin Fang,Qintong Li

Nature communications 15:5937 PubMed39009564

2024

Single-cell multiomics reveals ENL mutation perturbs kidney developmental trajectory by rewiring gene regulatory landscape.

Applications

Unspecified application

Species

Unspecified reactive species

Lele Song,Qinglan Li,Lingbo Xia,Arushi Eesha Sahay,Qi Qiu,Yuanyuan Li,Haitao Li,Kotaro Sasaki,Katalin Susztak,Hao Wu,Liling Wan

Nature communications 15:5731 PubMed38977708

2024

Targeting the transmembrane cytokine co-receptor neuropilin-1 in distal tubules improves renal injury and fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yinzheng Li,Zheng Wang,Huzi Xu,Yu Hong,Mengxia Shi,Bin Hu,Xiuru Wang,Shulin Ma,Meng Wang,Chujin Cao,Han Zhu,Danni Hu,Chang Xu,Yanping Lin,Gang Xu,Ying Yao,Rui Zeng

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2402066 PubMed38940381

2024

Uremic Toxin Receptor AhR Facilitates Renal Senescence and Fibrosis via Suppressing Mitochondrial Biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Xie,Ninghao Yang,Li Lu,Xi'ang Sun,Jingyao Li,Xin Wang,Hengjiang Guo,Li Zhou,Jun Liu,Huijuan Wu,Chen Yu,Wei Zhang,Limin Lu

Life medicine 2:lnad047 PubMed39872058

2023

Generation of renal tubular organoids from adult SOX9 kidney progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dewei Zhou,Dandan Li,Hao Nie,Jun Duan,Sarah Liu,Yujia Wang,Wei Zuo

International journal of molecular sciences 24: PubMed37511521

2023

Esaxerenone Inhibits Renal Angiogenesis and Endothelial-Mesenchymal Transition via the VEGFA and TGF-β1 Pathways in Aldosterone-Infused Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomeng Gao,Jingyue Chang,Yi Chang,Lili Fan,Ziqian Liu,Cuijuan Zhang,Tatsuo Shimosawa,Fan Yang,Qingyou Xu

eLife 12: PubMed36722887

2023

Neutral amino acid transporter SLC38A2 protects renal medulla from hyperosmolarity-induced ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chunxiu Du,Hu Xu,Cong Cao,Jiahui Cao,Yufei Zhang,Cong Zhang,Rongfang Qiao,Wenhua Ming,Yaqing Li,Huiwen Ren,Xiaohui Cui,Zhilin Luan,Youfei Guan,Xiaoyan Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLC12A3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB95302)

Western blot - Anti-SLC12A3 antibody (AB95302)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

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