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Rabbit Recombinant Monoclonal SLC12A3 antibody. Suitable for Dot, IHC-P, WB and reacts with Transfected cell lysate - Human, Human, Mouse, Rat samples.

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Images

Multiplex immunohistochemistry - Anti-SLC12A3 antibody [EPR27106-48] (AB316191), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-SLC12A3 antibody [EPR27106-48] (AB316191), expandable thumbnail
  • Western blot - Anti-SLC12A3 antibody [EPR27106-48] (AB316191), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (AB316191), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (AB316191), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotIHC-PWBICC/IFFlow Cyt (Intra)IP
Human
Expected
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Expected
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Expected
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Transfected cell lysate - Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human, Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Transfected cell lysate - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Transfected cell lysate - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Transfected cell lysate - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Transfected cell lysate - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Transfected cell lysate - Human

Dilution info

-

Notes

-

Target data

Function

Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of sodium and chloride reabsorption in kidney distal convoluted tubules (PubMed:18270262, PubMed:21613606, PubMed:22009145, PubMed:36351028, PubMed:36792826). Also acts as a receptor for the pro-inflammatory cytokine IL18, thereby contributing to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2 (By similarity). May act either independently of IL18R1, or in a complex with IL18R1 (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal SLC12A3 antibody. Suitable for Dot, IHC-P, WB and reacts with Transfected cell lysate - Human, Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR27106-48

Purification technique

Affinity purification Protein A

Specificity

Not suitable for Human WB and Rat WB.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The SLC12A3 gene encodes the thiazide-sensitive sodium-chloride co-transporter (NCC) also known as solute carrier family 12 member 3. This protein plays an important role in sodium and chloride ion transport across cell membranes in the distal convoluted tubule of the nephron in the kidney influencing electrolyte and fluid balance. It is a membrane protein with a mass of about 108 kDa. SLC12A3 is highly expressed in the kidney specifically localized in the distal convoluted tubule where it contributes to ions balance.

Biological function summary

The activity of SLC12A3 regulates blood pressure and volume homeostasis. It is an integral part of ion transport mechanisms and operates within a network of other transporters and channels in the kidney. The proper function of this transporter balances sodium and chloride reabsorption preventing excessive loss or retention. While not directly forming a complex its activity correlates with the function of other ion transport proteins in maintaining ion balance.

Pathways

The SLC12A3 protein integrates into the renal system's regulation of electrolyte homeostasis. It interacts within the salt-sensitive pathway by influencing the reabsorption of sodium chloride which impacts the renin-angiotensin-aldosterone system (RAAS) indirectly. The activity of SLC12A3 influences blood pressure regulation connecting it functionally with proteins like WNK kinases which modulate its phosphorylation state altering its transport capacity.

Associated diseases and disorders

SLC12A3 mutations lead to Gitelman syndrome a kidney disorder causing hypokalemic alkalosis hypomagnesemia and low blood pressure. Individuals with this disorder have impaired function of the SLC12A3 protein resulting in improper sodium and chloride reabsorption. The disorder connects with the renin-angiotensin pathway which tries to compensate by increasing renin production. It contrasts with conditions involving the CFTR protein which affects ion transport in diseases such as cystic fibrosis but focuses on chloride channels rather than co-transporters.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

  • Multiplex immunohistochemistry - Anti-SLC12A3 antibody [EPR27106-48] (ab316191), expandable thumbnail

    Multiplex immunohistochemistry - Anti-SLC12A3 antibody [EPR27106-48] (ab316191)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining GLEPP1/PTPRO with Anti-GLEPP1/PTPRO antibody [EPR28645-553] ab322047 at a 1:2000 (0.261 ug/ml) dilution, Anti-SGLT2 antibody [EPR29167-46] ab320086 anti-SLC5A2 used at 1:1000 (0.508 ug/ml) dilution and ab316191 anti-SLC12A3 used at a 1:100 (5.08 ug/ml) dilution.

    Panel A: merged staining of anti-PTPRP (magenta; Opal™690), anti-SLC5A2 (green; Opal™520) and anti-SLC12A3 (gray; Opal™570) on human kidney.

    Panel B: anti-PTPRP staining glomerulus in human kidney.

    Panel C: anti-SLC5A2 staining proximal tubules in human kidney.

    Panel D: anti-SLC12A3 staining distal tubules in human kidney.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-GLEPP1/PTPRO antibody [EPR28645-553] ab322047, Anti-SGLT2 antibody [EPR29167-46] ab320086 and ab316191 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-SLC12A3 antibody [EPR27106-48] (ab316191), expandable thumbnail

    Multiplex immunohistochemistry - Anti-SLC12A3 antibody [EPR27106-48] (ab316191)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse kidney tissue staining GLEPP1/PTPRO with Anti-GLEPP1/PTPRO antibody [EPR28645-553] ab322047 at a 1:2000 (0.261 ug/ml) dilution, ab316191 anti-SLC12A3 used at 1:2000 (0.254 ug/ml) dilution and Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] ab254222 anti-Angiotensin Converting Enzyme 1 used at a 1:4000 (0.141 ug/ml) dilution.

    Panel A: merged staining of anti-PTPRO (magenta; Opal™690), anti-SCL12A3 (green; Opal™520) and anti-Angiotensin Converting Enzyme 1 (gray; Opal™570) on mouse kidney.

    Panel B: anti-PTPRO staining glomerulus in mouse kidney.

    Panel C: anti-SCL12A3 staining distal tubules in mouse kidney.

    Panel D: anti-Angiotensin Converting Enzyme 1 staining proximal tubules in mouse kidney.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-GLEPP1/PTPRO antibody [EPR28645-553] ab322047, ab316191 and Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] ab254222 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-SLC12A3 antibody [EPR27106-48] (ab316191), expandable thumbnail

    Western blot - Anti-SLC12A3 antibody [EPR27106-48] (ab316191)

    Negative control: lung (PMID: 35591852), liver (PMID: 35591852).

    Samples are non-boiled as boiling may cause protein aggregation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-SLC12A3 antibody [EPR27106-48] (ab316191) at 1/1000 dilution

    Lane 1: Mouse kidney tissue lysate at 20 µg

    Lane 2: Mouse liver tissue lysate at 20 µg

    Lane 3: Mouse lung tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution

    Observed band size: 150 kDa, 36 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling SLC12A3 with ab316191 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on rat liver.

    The section was incubated with ab316191 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SLC12A3 with ab316191 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on rat cerebrum.

    The section was incubated with ab316191 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SLC12A3 with ab316191 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on mouse liver.

    The section was incubated with ab316191 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SLC12A3 with ab316191 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on mouse cerebrum.

    The section was incubated with ab316191 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling SLC12A3 with ab316191 at 1/100 (5.08 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on human liver (PMID: 8812482).

    The section was incubated with ab316191 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling SLC12A3 with ab316191 at 1/100 (5.08 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on human cerebrum (PMID: 8812482).

    The section was incubated with ab316191 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling SLC12A3 with ab316191 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on distal tubules of rat kidney (PMID: 21865292).

    The section was incubated with ab316191 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling SLC12A3 with ab316191 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on distal tubules of mouse kidney (PMID: 26337786).

    The section was incubated with ab316191 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody [EPR27106-48] (ab316191)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SLC12A3 with ab316191 at 1/100 (5.08 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on distal tubules of human kidney (PMID: 33328404).

    The section was incubated with ab316191 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Dot Blot - Anti-SLC12A3 antibody [EPR27106-48] (ab316191), expandable thumbnail

    Dot Blot - Anti-SLC12A3 antibody [EPR27106-48] (ab316191)

    Dot blot analysis of SLC12A3 using ab316191 at 1:1000 (0.508 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    This antibody does not cross-react with human SLC12A1 and human SLC12A2.

    All lanes: Dot Blot - Anti-SLC12A3 antibody [EPR27106-48] (ab316191) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 10 µg

    Lane 2: 293T cells transfected with a SLC12A3 expression vector containing a His-tag, whole cell lysate at 10 µg

    Lane 3: 293T cells transfected with a SLC12A1 expression vector containing a His-tag, whole cell lysate at 10 µg

    Lane 4: 293T cells transfected with a SLC12A2 expression vector containing a His-tag, whole cell lysate at 10 µg

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 169s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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