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AB254039

Anti-SLC12A3 (phospho T53) antibody

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(2 Publications)

Rabbit Polyclonal SLC12A3 phospho T53 antibody. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Slc12a3 phospho T53.

View Alternative Names

Ncc, Tsc, Slc12a3, Solute carrier family 12 member 3, Na-Cl symporter, Thiazide-sensitive sodium-chloride cotransporter

1 Images
Western blot - Anti-SLC12A3 (phospho T53) antibody (AB254039)
  • WB

Supplier Data

Western blot - Anti-SLC12A3 (phospho T53) antibody (AB254039)

Band of interest smearing likely due to glycosylation.

All lanes:

Western blot - Anti-SLC12A3 (phospho T53) antibody (ab254039) at 1/1000 dilution

Lane 1:

Mouse kidney lysate

Lane 2:

Mouse kidney lysate, treated with lambda phosphatase (1200 units for 30 mins)

Predicted band size: 113 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Mouse Slc12a3 phospho T53. The exact immunogen used to generate this antibody is proprietary information.

P59158

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Storage buffer
pH: 7.5 Constituents: HEPES, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.01% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SLC12A3 gene encodes the thiazide-sensitive sodium-chloride co-transporter (NCC) also known as solute carrier family 12 member 3. This protein plays an important role in sodium and chloride ion transport across cell membranes in the distal convoluted tubule of the nephron in the kidney influencing electrolyte and fluid balance. It is a membrane protein with a mass of about 108 kDa. SLC12A3 is highly expressed in the kidney specifically localized in the distal convoluted tubule where it contributes to ions balance.
Biological function summary

The activity of SLC12A3 regulates blood pressure and volume homeostasis. It is an integral part of ion transport mechanisms and operates within a network of other transporters and channels in the kidney. The proper function of this transporter balances sodium and chloride reabsorption preventing excessive loss or retention. While not directly forming a complex its activity correlates with the function of other ion transport proteins in maintaining ion balance.

Pathways

The SLC12A3 protein integrates into the renal system's regulation of electrolyte homeostasis. It interacts within the salt-sensitive pathway by influencing the reabsorption of sodium chloride which impacts the renin-angiotensin-aldosterone system (RAAS) indirectly. The activity of SLC12A3 influences blood pressure regulation connecting it functionally with proteins like WNK kinases which modulate its phosphorylation state altering its transport capacity.

SLC12A3 mutations lead to Gitelman syndrome a kidney disorder causing hypokalemic alkalosis hypomagnesemia and low blood pressure. Individuals with this disorder have impaired function of the SLC12A3 protein resulting in improper sodium and chloride reabsorption. The disorder connects with the renin-angiotensin pathway which tries to compensate by increasing renin production. It contrasts with conditions involving the CFTR protein which affects ion transport in diseases such as cystic fibrosis but focuses on chloride channels rather than co-transporters.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of sodium and chloride reabsorption in kidney distal convoluted tubules (PubMed : 15068971, PubMed : 17488636, PubMed : 19633012, PubMed : 36370249). Also acts as a receptor for the pro-inflammatory cytokine IL18, thereby contributing to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2 (PubMed : 26099046). May act either independently of IL18R1, or in a complex with IL18R1 (PubMed : 26099046).
See full target information Slc12a3 phospho T53
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 16:905403 PubMed35860293

2022

Ceftriaxone Suppresses Group II Metabotropic Glutamate Receptor Expression Contributing to Reversal of Recognition Memory Deficits of Amyloid Precursor Protein/Presenilin 1 AD Mice.

Applications

Unspecified application

Species

Unspecified reactive species

ShuJuan Fan,Li Li,LiRong Liu,He Li,XiaoHui Xian,WenBin Li

International journal of molecular sciences 22: PubMed33498219

2021

MST3 Involvement in Na and K Homeostasis with Increasing Dietary Potassium Intake.

Applications

Unspecified application

Species

Unspecified reactive species

Chee-Hong Chan,Sheng-Nan Wu,Bo-Ying Bao,Houng-Wei Li,Te-Ling Lu
View all publications
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