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AB241348

Anti-SLC12A6/KCC3 antibody

3

(1 Review)

|

(1 Publication)

Rabbit Polyclonal SLC12A6/KCC3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC12A6 aa 1-100.

View Alternative Names

KCC3, SLC12A6, Solute carrier family 12 member 6, Electroneutral potassium-chloride cotransporter 3, K-Cl cotransporter 3

1 Images
Western blot - Anti-SLC12A6/KCC3 antibody (AB241348)
  • WB

Supplier Data

Western blot - Anti-SLC12A6/KCC3 antibody (AB241348)

Prepared using RIPA buffer.

All lanes:

Western blot - Anti-SLC12A6/KCC3 antibody (ab241348) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 128 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SLC12A6 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9UHW9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to SLC12A6/KCC3 immobilized on solid support
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC12A6 also known as KCC3 is a potassium-chloride cotransporter involved in regulating ion homeostasis. The protein is big weighing approximately 135 kDa. It moves potassium and chloride ions across cell membranes which affects osmotic balance and cell volume. KCC3 is found widely in the human body with higher expression in the nervous system kidneys and heart. This wide expression suggests its important role in maintaining balance of ions in various tissues.
Biological function summary

KCC3 impacts cellular processes by being an important part of regulating salt balance within cells. It belongs to the cation-chloride cotransporter family and plays an important role in cell volume regulation. KCC3 does not usually function as part of a larger protein complex but acts as a standalone transporter. It helps stabilize cell environment which is necessary for correct cellular signaling and function.

Pathways

KCC3 is integral to maintaining the proper function of the WNK-SPAK/OSR1 kinase pathway. This pathway regulates ion transport and adjusts cell volume after osmotic stress. KCC3 interacts with proteins from this pathway like WNK kinases and SPAK which phosphorylate KCC3 to control its activity. As the transporter modifies ion flow it indirectly affects other signaling pathways dependent on ionic balance within cells.

Mutations in KCC3 link to Hereditary Motor and Sensory Neuropathy with Agenesis of the Corpus Callosum (HMSN/ACC). This disorder affects nerve function indicating KCC3’s role in nervous system health. Additionally KCC3 associates with epilepsy where imbalanced ion homeostasis disrupts neuron function. Related proteins such as WNK kinases when dysregulated have implications for these conditions as well highlighting the complex network KCC3 is involved in.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. Mediates electroneutral potassium-chloride cotransport when activated by cell swelling (PubMed : 10600773, PubMed : 11551954, PubMed : 16048901, PubMed : 18566107, PubMed : 19665974, PubMed : 21628467, PubMed : 27485015). May contribute to cell volume homeostasis in single cells (PubMed : 16048901, PubMed : 27485015).. Isoform 2. Mediates electroneutral potassium-chloride cotransport when activated by cell swelling (PubMed : 16048901, PubMed : 33199848, PubMed : 34031912). May contribute to cell volume homeostasis in single cells (Probable).. Isoform 3. Mediates electroneutral potassium-chloride cotransport when activated by cell swelling (PubMed : 16048901). May contribute to cell volume homeostasis in single cells (Probable).. Isoform 4. Mediates electroneutral potassium-chloride cotransport when activated by cell swelling (PubMed : 16048901). May contribute to cell volume homeostasis in single cells (Probable).. Isoform 5. Mediates electroneutral potassium-chloride cotransport when activated by cell swelling (PubMed : 16048901). May contribute to cell volume homeostasis in single cells (Probable).. Isoform 6. Mediates electroneutral potassium-chloride cotransport when activated by cell swelling (PubMed : 16048901). May contribute to cell volume homeostasis in single cells (Probable).
See full target information SLC12A6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Brain communications 4:fcac102 PubMed35602653

2022

Disruption of cellular iron homeostasis by missense variants causes severe neurodevelopmental delay, dystonia and seizures.

Applications

Unspecified application

Species

Unspecified reactive species

Nunziata Maio,Russell P Saneto,Richard Steet,Marcio A Sotero de Menezes,Cindy Skinner,Tracey A Rouault
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